Peppermint (Mentha piperita L.) essential oil as a potent anti-inflammatory, wound healing and anti-nociceptive drug

  • Sarah Kehili Laboratory of Research on Bio-Active Products and Valorization of Biomass, Department of Chemistry, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
  • Mohamed Nadjib Boukhatem Department of Biology and Cell Physiology, Faculty of Life and Natural Sciences, University - Saad Dahab - Blida 1, B.P. 270, Blida, Algeria; Research Laboratory of “Ethnobotany and Natural Products”, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
  • Asma Belkadi Laboratory of Pharmacology and Toxicology, Research and Development Center, SAIDAL Pharmaceutical, Gué de Constantine, Algiers, Algeria
  • Mohamed Amine Ferhat Laboratory of Research on Bio-Active Products and Valorization of Biomass, Department of Chemistry, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
  • William N. Setzer Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA; Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA
Keywords: Topical anti-inflammatory, Peppermint essential oil, Menthol, Anti-nociceptive activity, Wound healing


The present investigation was designed to study the chemical composition of Algerian peppermint essential oil (PEO) as well as the in vitro and in vivo anti-inflammatory, wound-healing and anti-nociceptive properties. Twenty-three compounds were identified in the PEO with the main chemical component as menthol (53.29%). Also, PEO showed a high content of oxygenated monoterpene compounds (92.75%). Topical application of PEO at doses of 200 and 20 µL/kg significantly reduced the acute ear edema in 38.09% and 36.50, respectively. Histological observation confirmed that PEO inhibited the skin inflammatory response. In-vivo wound healing activity of the cream prepared from PEO (0.5% w/w) was assessed by circular excision wound model followed by histological examination. The topical administration of PEO cream showed a significant decrease of unhealed wound area rate between the 6th (1.67±0.14 mm2) and the 9th (0.49±0.22 mm2) days of treatment when compared with the vehicle (2.32±0.77 mm2; p<0.05) and Madecassol® 0.1% creams (2.23±0.35 mm2; p<0.05). The PEO reduced nociceptive behavior at all doses tested in the acetic acid-induced nociception test (p<0.05). These findings support the anti-inflammatory, wound-healing and analgesic properties of PEO. We suggest that PEO is a promising candidate for use in skin care products with anti-inflammatory and wound-healing properties.



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How to Cite
Kehili, S.; Boukhatem, M.; Belkadi, A.; Ferhat, M.; Setzer, W. Peppermint (Mentha Piperita L.) Essential Oil As a Potent Anti-Inflammatory, Wound Healing and Anti-Nociceptive Drug. European Journal of Biological Research 2020, 10, 132-149.
Research Articles