European Journal of Biological Research http://www.journals.tmkarpinski.com/index.php/ejbr <p><strong>ISSN: 2449-8955</strong><br><strong><a title="MNiSW points: 11" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 11<br></a></strong><strong><a title="ICV: 100.00" href="http://journals.indexcopernicus.com/European+Journal+of,p24783440,3.html" target="_blank" rel="noopener">ICV 2018: 100.00</a></strong><br><strong>Acceptance rate: 2016 - 64%, 2017 - 56%</strong><br><strong>Google-based IF (2017): 0.586&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; h-index: 11</strong></p> en-US ejbr@tmkarpinski.lh.pl (Joanna Bródka) ejbr@tmkarpinski.lh.pl (Joanna Bródka) Wed, 29 Jan 2020 23:16:12 +0100 OJS 3.1.0.1 http://blogs.law.harvard.edu/tech/rss 60 Pseudomonas species from cattle dung producing extended spectrum and metallo beta-lactamases http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/237 <p>Indiscriminate use of antibiotics in livestock contributes to emergence of antimicrobial resistance in pathogens co-habiting the gastro-intestinal tract of animals. This study was to determine the Extended Spectrum Beta-Lactamase (ESBL) and Metallo-Beta-Lactamase (MBL) production in <em>Pseudomonas</em> species from cattle fecal samples. Cattle dungs were collected from the University of Ibadan Cattle Ranch and the <em>Pseudomonas</em> species isolated using <em>Pseudomonas</em> Base Agar with <em>Pseudomonas</em> CN Selective Supplement were identified using standard tests. Phenotypic detection of ESBL and MBL was by double disk synergy test and Ethylene Di-amine Tetra Acetic Acid Combined Disk Test respectively. Antibiotics susceptibility tests was done using the disc diffusion technique against ten antibiotics. A total of 144 <em>Pseudomonas </em>species were isolated and identified as<em> P. aeruginosa</em> (71.5%)<em>, P. fluorescens </em>(19.4%) and <em>P. stutzeri </em>(9.1%) and 19 (37.1%) produced ESBL including <em>P. aeruginosa </em>(15), <em>P. fluorescens </em>(2) and <em>P. stutzeri </em>(2) while, one (6.7%) ESBL <em>P. aeruginosa </em>produced MBL. All the ESBL producers were resistant to cefotaxime and trimethoprim; resistance of <em>P. aeruginosa</em> to ciprofloxacin was 93.3% and to ceftazidime was 80.0%, while it was 13.3% (colistin) and 6.7% (imipenem). The ESBL producing <em>P. fluorescens</em> were resistant to ceftazidime, ciprofloxacin and trimethoprim, likewise, the ESBL producing <em>P. stutzeri </em>showed resistance to gentamicin, ciprofloxacin and trimethoprim. The production of ESBL and MBL observed among the <em>Pseudomonas</em> species in this study with high level of resistance to some antibiotics portend public health risk, hence a need for caution in the use of antibiotics in animal husbandry.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3630802">http://dx.doi.org/10.5281/zenodo.3630802</a></strong></p> Olutayo Israel Falodun, Isaiah Baba Musa ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/237 Wed, 29 Jan 2020 23:17:40 +0100 An overview of floral and faunal diversity in and around Barrackpore Rastraguru Surendranath College Campuses, West Bengal, India http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/256 <p>The present survey based study involves the qualitative diversity of flora and fauna. The diversity assessment was carried out at two campuses of Barrackpore Rastraguru Surendranath College. This extensive study reveals the presence of 256 floral species and 165 faunal species in and around college campuses. The huge faunal diversity is mainly due to high level of floral diversity, which establishes the area as resource-rich habitat with promising reservoir of species. This is the very first effort in exploring the natural wealth of Barrackpore Rastraguru Surendranath College campuses.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3695917">http://dx.doi.org/10.5281/zenodo.3695917</a></strong></p> Monojit Ray, Sandip Pal ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/256 Tue, 03 Mar 2020 00:00:00 +0100 Production and optimization of polyhydroxybutyrate (PHB) from Bacillus megaterium as biodegradable plastic http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/243 <p>Among biodegradable plastics polyhydroxy alkanate and its polymers have received more attention than other biodegradable polymers because of their complete degradability, flexibility, water resistance and also the ease of production process. Polyhydroxybutyrate is one of the types of polyhydroxy alkanates that is seen as a storage granule in many microorganisms. In this study, <em>Bacillus megaterium</em> was prepared from Iranian microbial collection. Glucose and yeast extract were used as the main components of the medium in seed media 9 and 2.5 g/l and in fermentation medium 30 and 7.5 g/l respectively. GC-MASS and FTIR were used to identify the PHB produced. The results showed that the highest amount of biomass (0.221 g/l) and PHB (0.080 g/l) were obtained with glucose at 37°C and shaker speed of 150 rpm for 72 h incubation. The results of GC MASS and FTIR showed the production of PHB by <em>Bacillus</em> under investigation. Based on the mean of data on total cell growth conditions, the rate of cell biomass and PHB production in <em>B. megaterium</em> were 0.0869 and 0.0171 respectively. According to the results of the experiments, temperature had the greatest effect on biomass production and PHB production. The bioplastics produced by microbes are also highly degradable in the environment, and due to their specific chemical structure, they have been widely used in various fields of the food, pharmaceutical and chemical industries and are likely to replace today's plastics in the near future.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3711400">http://dx.doi.org/10.5281/zenodo.3711400</a></strong></p> Salman Ahmady-Asbchin, Hassan Rezaee, Moein Safari, Pantea Zamanifar, Davood Siyamiyan ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/243 Sun, 15 Mar 2020 15:11:17 +0100 Phycoremediation of water contaminated with arsenic (As), cadmium (Cd) and lead (Pb) from a mining site in Minna, Nigeria http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/251 <p>This study was designed to remediate water contaminated with heavy metals (arsenic, cadmium and lead) using two green macroalgal species, <em>Spirogyra</em> and <em>Cladophora</em>. The results obtained from this study indicate that both macroalgae can be employed to adsorb and detoxify any of the three heavy metals from aqueous solution. However, it was also discovered from the study that <em>Cladophora </em>adsorbed and detoxified more of the cadmium and lead than arsenic as the organism had removal efficiency for cadmium and lead as 88.78% and 94.85% respectively meanwhile for arsenic it was only 23.10%. On the otherhand however, <em>Spirogyra</em> adsorbed more of arsenic than cadmium and lead as the organism had a record of 82.76% of arsenic compared to the 28.97% and 47.43%absorption forcadmium and lead respectively. It is therefore concluded based on the results of the present study that reclamation and reuse of water from public or industrial wastewater, or even from water contaminated as a result of precious metal mining is a huge possibility through the application of phycoremediation, using different species of micro and macroalgae.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.3735302">http://dx.doi.org/10.5281/zenodo.3735302</a></strong></p> Olabisi Peter Abioye, Basil Utazi Ezugwu, Sesan Abiodun Aransiola, Musa Innocent Ojeba ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/251 Tue, 31 Mar 2020 00:00:00 +0200