European Journal of Biological Research http://www.journals.tmkarpinski.com/index.php/ejbr <p><strong>ISSN: 2449-8955</strong><br><strong><a title="MNiSW points: 11" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 11<br></a></strong><strong><a title="ICV: 100.00" href="http://journals.indexcopernicus.com/European+Journal+of,p24783440,3.html" target="_blank" rel="noopener">ICV 2017: 100.00</a></strong><br><strong>Acceptance rate: 2016 - 64%, 2017 - 56%</strong><br><strong>Google-based IF (2017): 0.586&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; h-index: 11</strong></p> TMKarpinski Publisher en-US European Journal of Biological Research 2449-8955 Therapeutic and pharmacological aspects of photodynamic product chlorophyllin http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/118 <p>Medicinal plants have been used for thousands of years to flavor and conserve food, to treat health disorders and to prevent diseases including epidemics. They can provide biologically active molecules and lead structures for development of modified derivatives with enhanced activity or reduced activity.&nbsp; The isolation and identification of active principles and elucidation of the mechanism of action of a drug is of paramount importance. One such compound is chlorophyllin, a water soluble analogue of the ubiquitous green pigment chlorophyll. It acts as an effective inhibitor of aflatoxin hepatocarcinogenesis in animal models by blocking carcinogen bioavailability. Further anti-cancer effects of chlorophyllin including antioxidant activity, inhibition of enzymatic activity that converts inert procarcinogens into active carcinogens, stimulation of enzymatic activity that promotes the elimination of toxic substances from the body and antitumor activity have likewise been evidenced by controlled studies. Phytotherapy of snails by photodynamic chlorophyllin is a new approach to control the epidemic fasciolosis. Photosensitive chlorophyllin is degraded very fast without the formation of toxic byproducts, therefore, it is environmentally sound and economically safe also.</p> <p>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2638869">http://dx.doi.org/10.5281/zenodo.2638869</a></p> Divya Chaturvedi Kavita Singh Vinay Kumar Singh ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-04-13 2019-04-13 9 2 64 76 Screening for antifungal activity of garlic (Allium sativum) powder against mycelia growth of three post-harvest pathogens http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/122 <p>Screening for antifungal activity of garlic powder against mycelia growth of three post-harvest pathogens (<em>Aspergillus</em>, <em>Rhizopus</em> and <em>Mucor</em> species) was investigated in this study. Five grams of malt extract agar (MEA) were poured into a conical flask, 100 ml of water and different weight of garlic powder (1, 3, 5 and 7 g) were separately added, stirred and later sterilized while MEA medium with no garlic added (0 g) served as control. The mycelia of each post-harvest pathogen was cut with 6mm cork borer and placed on the solidified medium in the Petri dish and incubated at 28±2<sup>o</sup>C for 72 hours. Phytochemical screening of the garlic powder was also investigated. Results from this study showed that the different weights of the garlic powder apart from the control (0 g garlic) significantly inhibited the mycelia growth of the three post-harvest pathogens tested in the study and the order of antifungal activity of the garlic powder against mycelia growth of <em>Aspergillus,</em> <em>Rhizopus</em> and <em>Mucor</em> species&nbsp; was 7 g &gt; 5 g &gt; 3 g &gt; 1 g &gt; 0 g, 5 g &gt; 7 g &gt;1 g &gt; 3 g &gt; 0 g and&nbsp; 7 g &gt; 5 g &gt; 3 g &gt; 1 g &gt; 0 g respectively. The antifungal activity of the garlic powder may be related to the presence of active antimicrobial agents including alkaloids, saponins, tannins, flavonoids and cardiac glycosides that were detected in the powder.</p> <p>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2635824">http://dx.doi.org/10.5281/zenodo.2635824</a></p> Oluwole Olakunle Oladele ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-04-11 2019-04-11 9 2 57 63 Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/168 <p>Here was investigated the incorporation of copper in MS medium on growth, and metabolic activities of <em>Solanum nigrum</em> callus. Copper up to 75 µM increased the growth, and thereafter a decline was observed. No considerable alteration in MDA, H<sub>2</sub>O<sub>2</sub>, bound phenolics, flavonoids, ascorbate, and copper content was observed with the existence of 25 µM copper, then levels of these parameters were raised with rising copper concentrations. Similarly, 25 µM copper didn't induce a considerable change in lipoxygenase, superoxide dismutase, catalase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase activities, however, high levels stimulated these enzymes. Copper at 25 µM didn’t considerably reduce amino acids and soluble proteins, whereas higher concentrations reduced these parameters. Copper treatments reduced the soluble carbohydrates accumulation; only 75 µM enhanced this accumulation. Copper at 25 µM significantly increased the potassium accumulation, whereas higher concentrations reduced this accumulation. From these results, it might be contemplated the optimum effect concerning copper.</p> <p><strong>DOI: <a href="http://dx.doi.org/10.5281/zenodo.2848646">http://dx.doi.org/10.5281/zenodo.2848646</a></strong></p> Nasim A. R. M. Othman ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2019-05-15 2019-05-15 9 2 77 92