Efficacy of cloves (Syzygium aromaticum) and ginger (Zingiber officinale) extracts against microbial spoilage of locally produced soymilk at ambient storage

  • Oluwole Olakunle Oladele Storage Microbiology Laboratory, Department of Biology, School of Life Sciences, Federal University of Technology, PMB 704, Akure, Ondo State, Nigeria
  • Toyin Temitope Odeyemi Storage Microbiology Laboratory, Department of Biology, School of Life Sciences, Federal University of Technology, PMB 704, Akure, Ondo State, Nigeria
Keywords: Soymilk, Plant extracts, Biopesticide, Microorganisms, Bioactive, GC-MS

Abstract

There is a global concern at present with the use of synthetic chemical preservatives in foods due to carcinogenic and mutagenic related problems, resulting to the use of natural preservatives especially culinary herbs and spices in food products. Herein, efficacy of clove (Syzygium aromaticum) fruit and ginger (Zingiber officinale) rhizome was evaluated against rapid deterioration of locally produced soymilk at ambient storage by microorganisms and their bioactive compounds determined with gas chromatography and mass spectrometry (GC-MS). Interestingly, soymilk treated with 5% ginger extract among other tested concentrations had the least bacterial (2.00±0.50 ×104 cfu/ ml) and fungal (2.00±0.00 ×104 sfu/ml) counts that did not exceed the acceptable limit for pasteurized milk (3 ×104 cfu/ ml) by day 3 of storage when compared with untreated soymilk that recorded very high bacteria (56.50±6.50 ×104 cfu/ml) and fungi (13.50±1.50 ×104 sfu/ml) counts. The efficacy could be traced to beta-amyrin (24.15%), hexadecanoic acid (15.71%) and phytol (16.55%) that are major bioactive compounds detected in the ginger extract. Thus, the ginger extract offers prospect as promising alternative that can be applied in extending the shelf life of soymilk and other liquid processed foods.

DOI: http://dx.doi.org/10.5281/zenodo.14572313

Downloads

Download data is not yet available.

References

1. Li J, Hsieh YHP. Traditional Chinese food technology and cuisine. Asia Pacif J Clin Nutr. 2004; 13: 147-155.
2. Kolapo AL. Soybean: African’s Potential Cinderella Food Crop. Soybean: Biochemistry, Chemistry and Physiology. InTech Publications, Rijeka, Croatia, 2011; 137-150.
3. Adebayo-Tayo BC, Adegoke AA, Akinjogunla OJ. Microbial and Physico-Chemical Quality of Powdered Soymilk Samples in Akwa Ibom, South Southern Nigeria. Afr J Biotechnol. 2008; 8: 3066-3071.
4. Momoh JE, Udobi CE, Orukotan AA. Improving the Microbial Keeping Quality of Home-Made Soymilk Using a Combination of Preservatives, Pasteurization and Refrigeration. Brit J Dairy Sci. 2011; 2(1): 1-4.
5. Quigley L, O’Sullivan O, Stanton C, Beresford TP, Ross RP, Fitzgerald GF, Cotter PD. The Complex Microbiota of Raw Milk: Review. FEMS Microbiol Rev J. 2013; 37(5): 664-698.
6. Seetharamaiah K, Anton Smith A, Murali R, Manavalan R. Preservatives in Food Products- Review. Int J Phar Biol Arch. 2011; 2: 583-599.
7. Poljsak B, Kovac V, Milisav I. Antioxidants, Food Processing and Health. J Food Sci. 2021; 10(3): 433.
8. Sanjay S. Food preservatives and their harmful effects. Int J Sci Res Publ. 2015; 5: 2250-3153.
9. Calo JR, Crandall PG, Corliss A, Steven CR. Review: Essential Oils as Antimicrobials in Food Systems. J Food Cons. 2015; 54: 111-119.
10. Tsao R, Akhtar MH. Nutraceuticals and functional foods: Current trend in phytochemical antioxidant research. J Agric Food Chem Env. 2005; 3: 10-17.
11. Bondi M, Laukova A, Niederhaunsern S, Messi P, Papadopoulou C. Natural Preservatives to Improve Food Quality and Safety. J Food Qual. 2017; 17: 1-3.
12. Iwe MO, Agu IF. Use of Natural Flavourant to Improve Soymilk Acceptability. Educational Publications Nigeria, 2003; 16-24.
13. Buchanan RE, Gibbons NE. Bergey’s Manual of Determinative Bacteriology (8. edn). Wilkins, Baltimore, 1974; 1268.
14. Frazier WC, Westhoff DC. Food Microbiology (4. edn.). Tata McGrawHill Publication Singapore, 1998; 115-238.
15. Lovestead TM, Urness K. Gas Chromatography-Mass Spectrometry (GC-MS). ASM Handbook, UK, 2019.
16. Adeniyi PO, Ajayi K. Preservation of the Nutritional Quality of Soymilk by Heat Treatment. Am J Food Nutr. 2020; 8(2): 40-46.
17. Adeleke OE, Adeniyi BA, Akinrinmisi AA. Microbiological Quality of Local Soymilk: A Public Health Appraisal. Afr J Biomed Res. 2000; 3: 89-92.
18. Tajkarimi M, Ibrahim S, Cliver DO. Antimicrobial Herb and Spice Compounds in Food. J Food Consum. 2010; 21(9): 1199-1218.
19. Gottardi D, Bukvicki D, Prasad S, Tyagi AK. Beneficial Effects of Spices in Food Preservation and Safety. Front Microbiol. 2016; 7(12): fmicb.2016.01394.
20. Wakoli AB, Onyango DAO, Rotich PJ. Effect of Selected Spices on Food Spoilage Rate. Global J Biol Agric Health Sci. 2014; 3(4): 160-162.
21. Kabiru YA, Makun HA, Saidu AN, Muhammad LH, Nuntah LC, Amoo SA. Soymilk Preservation Using Extracts of Cloves (Syzygium Aromaticum Myrtaceae) and Guinea-Pepper (Xylopia Aethiopica Annonaceae). J Pharm Biol Sci. 2012; 3(5): 44-50.
22. De Geyter E, Lambert E, Geelen D, Smagghe G. Novel advances with plant saponins as natural insecticides to control pest insect. Pest Technol. 2007; 9: 96-105.
23. Ngoci SN, Mwendia CM, Mwaniki CG. Phytochemical and cytotoxicity testing of Indigofera lupatana. J Animal Plant Sci. 2011; 11(1): 1364-1373.
24. Koushki M, Koohy-Kamaly P, Azizkhani M, Hadina N. Microbiological Quality of Pasteurized Milk on Expiration Iran J Dairy Sci. 2016; 99(3): 1796-1801.
25. Agboke AA, Uduma E, Osonwa C, Emmanuel O, Ibezim C. Evaluation of microbiology quality of some soybean milk products consumed in Nigeria. Prime J. 2011; 1: 25-30.
Published
2024-12-29
How to Cite
(1)
Oladele, O.; Odeyemi, T. Efficacy of Cloves (Syzygium Aromaticum) and Ginger (Zingiber Officinale) Extracts Against Microbial Spoilage of Locally Produced Soymilk at Ambient Storage. European Journal of Biological Research 2024, 14, 116-128.
Section
Research Articles