Statistical optimization as a powerful tool for indole acetic acid production by Fusarium oxysporum

  • Ghada Abd-Elmonsef Mahmoud Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
  • Hassan H. A. Mostafa Central Laboratory of Organic Agriculture, Agricultural Research Center, Giza 12619, Egypt
Keywords: Auxin, Production, Plackett-Burman, Fusarium

Abstract

Crop production is challenged in our world by increasing food demands, decrease natural resource bases and climatic change. Nowadays plant growth regulators works like fertilizers in increasing plant growth production efficiency and needed to produce in large industrial scale. Fermentation condition and medium constituents can significantly affect on the product production and designing an acceptable fermentation medium is critical importance. In this paper Fusarium sp. could be considered as promising indole-3-acetic acid producers with the ability to improve the production using statistical methods. The results showed that fermentation type, incubation temperature and L-tryptophan were the most influencing parameters on the production. Maximum IAA production by Fusarium oxysporum was 300.4 mg/l obtained under the fermentation conditions: temperature at 25oC, incubation period 5 days, pH 7, inoculums size 2%, shaking rate at 150 rpm and medium constituents: Glucose 40 g/l, yeast extract 3 g/l, L-tryptophan 1 g/l, KH2PO4 2 g/l, NaNO3 4 g/l, MgSO4·7H2O 0.1 g/l with regression analysis (R2) 99.67% and 2.12-fold increase in comparison to the production of the original level (142 mg/l).

DOI: http://dx.doi.org/10.5281/zenodo.1012348

Downloads

Download data is not yet available.

References

1. Hussain S, Peng S, Fahad S, Khaliq A. Rice management interventions to mitigate greenhouse gas emissions: a review. Environ Sci Pollut Res Int. 2015; 22(5): 3342-3360.

2. Fahad S, Hussain S, Matloob A, Khan FA. Phytohormones and plant responses to salinity stress: a review. Plant Growth Regul. 2015; 75(2): 391-404.

3. Iqbal N, Umar S, Khan NA, Khan MIR. A new perspective of phytohormones in salinity tolerance: regulation of proline metabolism. Environ Exp Bot. 2014; 100: 34-42.

4. Fahad S, Hussain S, Bano A, Saud S. Potential role of phytohormones and plant growth-promoting rhizobacteria in a biotic stresses: consequences for changing environment. Environ Sci Pollut Res. 2015; 22(7): 4907-4921.

5. Datta C, Basu P. Indole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub Cajanus cojan. Microbiol Res. 2000; 155: 123-127.

6. Teale WD, Paponov IA, Palme K. Auxin in action: signaling, transport and the control of plant growth and development. Mol Cell Biol. 2006; 7: 847-859.

7. Eyidogan F, Oz MT, Yucel M, Oktem HA. Signal transduction of phytohormones under a biotic stresses. In: Khan NA, Nazar R, Iqbal N, Anjum NA, eds. Phytohormones and a biotic stress tolerance in plants. Springer, Berlin, 2012: 1-48.

8. Takahashi H. Auxin biology in roots. Plant Root. 2013; 7: 49-64.

9. Mandal SM, Mondal KC, Dey S, Pati BR. Optimization of cultural and nutritional conditions for indole-3-acetic acid (IAA) production by a Rhizobium sp. isolated from root nodules of Vigna mungo (L.) Hepper. Res J Microbiol. 2007; 2: 239-246.

10. Thimann KV. Hydrolysis of indole acetonitrile in plants. J Biol Chem. 1953; 109: 279-291.

11. Wolf FT. The production of indole acetic acid by Ustilago zeae and its possible significant in tumor formation. Biochem. 1952; 38: 106-111.

12. Crady EE, Wolf FT. The production of indole acetic acid by Dibotryon morbosum and Taphrina deformans. Physiol Plant. 1959; 12: 526-533.

13. Perley JE, Stowe BB. On the ability of Taphrina deformans to produce indole acetic acid from tryptophan by way of tryptamine. Plant Physiol. 1966; 41: 234-237.

14. Riov RMJ, Sharon A. Indole-3-acetic acid biosynthesis in Colletotrichum gloeosporioides f. sp. Aeschynomene. Appl Environ Microbiol. 1998; 64: 5030-5032.

15. Hasan HA. Gibberellin and auxin-indole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol Immunol Hung. 2002; 49: 105-118.

16. Shilts CK, Timmer EL, Ueng PP. Indole derivatives produced by the fungus Colletotrichum acutatum causing lime anthracnose and postbloom fruit drop of citrus. FEMS Microbiology Lett. 2003; 226: 23-30.

17. Chung KR, Tzeng DD. Biosynthesis of indole-3-acetic acid by the gall-inducing fungus Ustilago esculenta. J Bio Sci. 2004; 4(6): 744-750.

18. Waqas M, Khan AL, Kamran M, Hamayun M. Endophytic fungi produce gibberellins and indole acetic acid and promotes host-plant growth during stress. Molec. 2012; 17: 10754-10773.

19. Xu C, Kim S, Hwang H, Choi J. Optimization of submerged culture conditions for mycelial growth and exobiopolymer production by Paecilomyces tenuipes C240. Process Biochem. 2003; 38(7): 1025-1030.

20. Aravindan R, Viruthagiri T. Sequential optimization of culture medium composition for extracellular lipase production by Bacillus sphaericus using statistical methods. J Chem Tec Bio. 2007; 82: 460-470.

21. Booth C. The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England; 1971.

22. Leslie JF, Summerell BA. The Fusarium Laboratory Manual. Blackwell Publishing; 2006.

23. Plackett RL, Burman JP. The design of optimum multifactorial experiments. Biometrika. 1947; 33: 305-325.

24. Gordon SA, Weber RP. Colorimetric estimation of indole acetic acid. Plant Phys. 1951; 26: 192-195.

25. Lynch JM. Origin, nature and biological activity of aliphatic substances and growth hormones found in soil. In: Vaughan D, Malcom RE, eds. Soil organic matter and biological activity. Martinus Nijhoff /Dr. W. Junk Publishers. Dordrecht, Boston, Lancaster, 1985: 151-174.

26. Thuler DS, Floh EI, Handro W, Barbosa HR. Beijerinckia derxii releases plant growth regulators and amino acids in synthetic media independent of nitrogenase activity. J Appl Microbiol. 2003; 95: 799-806.

27. Bilkay IS, Karako S, Aksöz N. Indole-3-acetic acid and gibberellic acid production in Aspergillus niger. Turk J Biol. 2010; 34: 313-318.

28. Yalçınkaya Y. Effects of some physiological conditions on indole-3-acetic acid production by Gibberella fujikuroi G5, MSc, Hacettepe University Institute of Science and Technology; 2007.

29. Gopinathan S, Raman N. Indole-3-acetic acid production by ectomycorrhizal fungi. Indian J Exp Biol. 1992; 30: 142-143.
Published
2017-12-31
How to Cite
(1)
Mahmoud, G.; Mostafa, H. Statistical Optimization As a Powerful Tool for Indole Acetic Acid Production by Fusarium Oxysporum. European Journal of Biological Research 2017, 7, 315-323.
Section
Research Articles

Most read articles by the same author(s)