MicroMedicine http://www.journals.tmkarpinski.com/index.php/mmed <p><strong>ISSN 2449-8947</strong><br><strong><a title="MNiSW points: 8" href="http://www.nauka.gov.pl/g2/oryginal/2016_12/c5c2fcb0c283a9eb3d1081020fd3178c.pdf" target="_blank" rel="noopener">MNiSW points 2016: 8</a></strong><br><a title="ICV: 86.27" href="http://journals.indexcopernicus.com/MicroMedicine,p24783445,3.html" target="_blank" rel="noopener"><strong>ICV 2015: 86.27</strong></a></p> TMKarpinski Publisher en-US MicroMedicine 2449-8947 Probiotics in colon cancer prevention http://www.journals.tmkarpinski.com/index.php/mmed/article/view/50 <p>Probiotics are live, selected microbial strains that have a beneficial effect on the human body and when introduced into the body, they colonize in the digestive tract, especially in the large intestine, exerting a beneficial effect on the health of the host. The microbial strains, so that they can be included in the probiotics, must be thoroughly tested and meet several conditions. These microorganisms multiply in the gastrointestinal tract and are competitive for pathogenic microorganisms that cause infection. Probiotic bacteria are found in natural yogurts, sour milk, sauerkraut, pickled cucumbers. Many studies show a positive correlation between the consumption of probiotics and the risk of developing certain cancers. Probiotics are most likely to reduce the risk of developing colorectal cancer.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1297660">http://dx.doi.org/10.5281/zenodo.1297660</a></p> Marlena Wolek Anna Bogacz Bogna Juskowiak Anna Polaszewska Bogusław Czerny ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-06-25 2018-06-25 6 2 62 68 Cucurbitaceae - the family that nourishes and heals http://www.journals.tmkarpinski.com/index.php/mmed/article/view/63 <p>A review of the activities of members of the Cucurbitaceae was carried out. Many of them are confirmed nutritious and therapeutical. Their global spread, diverse genera and phytochemical profile further confirm them as an attraction for the growth and survival of humanity. The need for alternative control measures to address resistance has heightened the passion for Cucurbitaceae in bioprospecting.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1436798">http://dx.doi.org/10.5281/zenodo.1436798</a></p> Stephen O. Fapohunda Aderiike A. Adewumi David O. Jegede ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-09-27 2018-09-27 6 2 85 93 Effect of Aloe vera gel on some haematological parameters and serum electrolytes in high salt loaded Wistar rats http://www.journals.tmkarpinski.com/index.php/mmed/article/view/43 <p>This study investigated the effect of <em>Aloe vera</em> gel on some haematological parameters and serum electrolytes in high salt loaded rats. Twenty (20) male Wistar rats (180-250 g) were randomly assigned into 4 groups (n=5): Control- received 0.2 ml normal saline; <em>Aloe</em>-received 600 mg/kg of <em>Aloe vera</em> gel orally once daily; Salt-fed (SF) received high salt diet (8% NaCl in feed + 1% NaCl in H<sub>2</sub>O); Saltfed-treated (SF+<em>Aloe</em>) received high salt diet + <em>Aloe vera</em> gel. All groups had access to rat feed and water throughout the duration (six weeks) of treatment. Blood samples were collected from each animal via cardiac puncture for analysis. Red blood cell (RBC) count, haemoglobin (Hb) concentration and packed cell volume (PCV) were significantly (p&lt;0.05) increased in SF and SF+<em>Aloe</em> groups compared with control and <em>Aloe</em> groups. Total white blood cell count was significantly (p&lt;0.001) decreased in SF group compared with control and <em>Aloe</em> groups and increased (p&lt;0.001) in SF+<em>Aloe</em> group compared with SF group. Neutrophil and lymphocyte counts were significantly increased and decreased respectively in SF+<em>Aloe</em> group compared with control (p&lt;0.01), <em>Aloe</em> (p&lt;0.05) and SF (p&lt;0.001) groups. Na<sup>+</sup>, K<sup>+</sup> and Cl<sup>-</sup> concentrations were significantly increased in SF and SF+<em>Aloe</em> group compared with control and <em>Aloe</em> groups. HCO<sub>3</sub><sup>-</sup> concentration was significantly increased in <em>Aloe</em> and SF+<em>Aloe</em> groups compared with control. High salt diet (HSD) caused alterations in red cell indices and posed threat to the immune system of rats. <em>Aloe vera</em> could not reverse these alterations but exhibited an immune-stimulatory effect. Both <em>Aloe vera</em> and HSD caused electrolyte imbalance.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1318287">http://dx.doi.org/10.5281/zenodo.1318287</a></p> Augustine Lishilinimye Udefa Archibong Nsa Archibong Ada Ak Akwari Solomon Ayaokpo Leilei ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-07-20 2018-07-20 6 2 69 77 Isolation of Enterobacteriaceae and non-fermenting Gram-negative bacilli (NFGNB) from Dental Unit Water Lines (DUWL) in a tertiary care institutional setup http://www.journals.tmkarpinski.com/index.php/mmed/article/view/38 <p><em>Background: </em>The quality of dental unit water lines (DUWL) is of considerable importance since patients and dental staff are regularly exposed to water and aerosols generated from dental units which thereby influence the individual patient outcome and health-care associated morbidity. The aim of the present study was to determine the microbiological quality of water used, presence of biofilms and also the potential of isolated bacterial species in producing biofilms within DUWL.</p> <p><em>Methods:</em> Thirty DUWL samples were collected from various departments of Manipal College of Dental Sciences, Mangalore. Bacteriological analysis was done for the presence of various bacterial contaminants. Presence of biofilms on DUWLs and potential of bacterial isolates to form biofilm were also determined.</p> <p><em>Results:</em> Seven of 30 samples (23.3%), were found to be of unsatisfactory quality (coliform count &gt; 200 CFU/ml), most frequently from air/water syringes. A total of 45 strains were isolated from 14 water samples. Genera isolated were <em>Escherichia</em> spp., <em>Enterobacter</em> spp., <em>Klebsiella</em> spp., <em>Pseudomonas</em> spp. and <em>Acinetobacter</em> spp. Four of 10 samples from DUWL tubing showed presence of biofilms (40%), formed mostly by <em>Acinetobacter</em> spp. and <em>Pseudomonas</em> spp. Out of 45 strains that were isolated, 19 strains displayed ability to form biofilms. Maximum number (10) isolates formed biofilms with 48 hours.</p> <p><em>Conclusion:</em> Exposure to contaminated water from DUWL poses threat to the well-being of the patient and the health care personnel as well. Hence, measures should be initiated to ensure the optimum quality of DUWL water.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1319772">http://dx.doi.org/10.5281/zenodo.1319772</a></p> A. Ebinesh Harshitha J. Naik - Shobha K. Vishwas Saralaya ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-07-23 2018-07-23 6 2 78 84 Effect of age on the Br, Fe, Rb, Sr, and Zn concentrations in human prostatic fluid investigated by energy-dispersive X-ray fluorescent microanalysis http://www.journals.tmkarpinski.com/index.php/mmed/article/view/80 <p>The effect of age on Br, Fe, Rb, Sr, and Zn concentrations in human prostatic fluid was investigated by <sup>109</sup>Cd radionuclide-induced energy dispersive X-ray fluorescent microanalysis. Specimens of expressed prostatic fluid were obtained from 51 men (mean age 51 years, range 18-82 years) with apparently normal prostates using standard rectal massage procedure. Mean values (M ± SΕΜ) for concentration of trace elements (mg·l<sup>-1</sup>) in human prostate fluid were: Br 3.58±0.59, Fe 9.04±1.21, Rb 1.10±0.08, Sr 1.08±0.17, and Zn 573±35. An age-related increase in Zn content and decrease in Br and Fe concentration was observed.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1447214">http://dx.doi.org/10.5281/zenodo.1447214</a></p> Vladimir Zaichick Sofia Zaichick ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-10-05 2018-10-05 6 2 94 104 Assessment of post intervention of geohelminth infection and risk factors among school aged children in the most endemic area of Kano, Nigeria http://www.journals.tmkarpinski.com/index.php/mmed/article/view/106 <p>This study is an initial attempt at determining the prevalence of geohelminth infections among school aged children (SAC) in the most endemic area of Kano State as well as risk factors associated with the infection and the impact of deworming programme in SAC. A retrospective study on the prevalence of geohelminth infection in the 44 Local Government Areas (LGA) of Kano State was conducted. A stratified random sampling technique was used for sample collection. A total of 3000 children were recruited aged 6-15 years. Retrospective study showed that none was of high endemicity. The present status of geohelminth showed that only hookworm was present among SAC with a prevalence of 2.2% and intensity was light (mean: 17 epg). Risk factors that predispose SAC to geohelminth infection like eating outside home, poor hand washing practice, and nail biting were found not to be significantly associated with hookworm infection except risk factor like walking bare footed which was significantly associated with hookworm infection. In conclusion, Prevalence of geohelminth infection in Kabo LGA prior to deworming was 35.1% and post intervention among SAC was generally low (2.2%). The observed low prevalence of geohelminth infection could be attributed to the success of the deworming programme carried out in the district in 2013. Risk factors like poor hand washing practice (2.3%), walking bare footed (2.6%) and eating outside home (2.6%) were pre-dominant among SAC.</p> <p><strong>DOI:</strong> <a href="http://dx.doi.org/10.5281/zenodo.1472466">http://dx.doi.org/10.5281/zenodo.1472466</a></p> Zainab Gobir T. I. Oyeyi B. Bello ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-10-26 2018-10-26 6 2 105 115