Chromatographic and antiproliferative assessment of the aerial root of Ficus thonningii Blume (Moraceae)

  • Maryam Odufa Adamu Department of Medicinal Plant Research and Traditional Medicine (MPR & TM), National Institute for Pharmaceutical Research and Development (NIPRD), Idu Industrial Area, P. M. B. 21, Garki, Abuja, Nigeria; Department of Chemistry, Nasarawa State University, Keffi, Nasarawa State, Nigeria
  • Solomon Fidelis Ameh Department of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Idu Industrial Area, P. M. B. 21, Garki, Abuja, Nigeria
  • Utibe Abasi Okon Ettah Department of Medicinal Plant Research and Traditional Medicine (MPR & TM), National Institute for Pharmaceutical Research and Development (NIPRD), Idu Industrial Area, P. M. B. 21, Garki, Abuja, Nigeria
  • Samuel Ehiabhi Okhale Department of Medicinal Plant Research and Traditional Medicine (MPR & TM), National Institute for Pharmaceutical Research and Development (NIPRD), Idu Industrial Area, P. M. B. 21, Garki, Abuja, Nigeria
Keywords: Ficus thonningii, Antiproliferative, β-sitosterol, Caffeic acid, Ferulic acid


Ficus thonningii (Blume) has long history of use for variety of ailments. The hot aqueous extract of Ficus thonningii aerial root (FT) was obtained by infusion. The antiproliferative activity of FT was evaluated using Sorghum bicolor seed radicle over a period of 24 h to 96 h. The mean radicle length (mm), percentage inhibition and percentage growth were calculated. Chemical characterization of FT was done using chromatographic techniques. Thin layer chromatography revealed the presence of β-sitosterol. High performance liquid chromatography showed ten peaks with gallic acid, tannins, caffeic acid, rutin, ferulic acid and morin eluting at 3.530, 3.928, 4.668, 6.706, 7.669 and 18.844 minutes respectively. Compared with negative control, FT at 1 mg/ml to 32 mg/ml significantly (p<0.0001) inhibited Sbicolor seed radicle growth over 24 h-96 h. At 96h, FT dose-dependently inhibited S. bicolor seed growth, giving a percentage inhibition of 20.31%, 24.30%, 31.71%, 53.23%, 78.74%, 95.37% at 1 mg/ml, 2 mg/ml, 4 mg/ml, 8 mg/ml, 16 mg/ml, 32 mg/ml, respectively. Methotrexate 50 µg/ml used as the positive control gave inhibition of 70.62% at 96h. The result revealed the potential of FT to inhibit rapid proliferating cells of S. bicolor seed radicle and by extension cancer cells.



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How to Cite
Adamu, M., Ameh, S., Ettah, U., & Okhale, S. (2018). Chromatographic and antiproliferative assessment of the aerial root of Ficus thonningii Blume (Moraceae). MicroMedicine, 6(1), 1-9. Retrieved from
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