Antimicrobial activity of crude henna extract against Gram-positive bacteria

  • Samira M. S. Ibrahim College of Nursing, University of Duhok, Duhok, Iraq
  • Chiman S. Rasool Department of Pathology, College of Nursing, University of Duhok, Duhok, Iraq
  • Asaad Ab. Al-Asady College of Medicine, University of Duhok, Duhok, Iraq
Keywords: Lawsonia inermis, Extract, Antimicrobial activity, Medicinal plant, Henna


Henna plant has been used in herbal medicine for ages, but the medical uses of this plant as antimicrobial agent had not been well discussed. In this study we aim to examine the effect of ethanolic extract of local Basra henna leaves on Gram-positive bacteria species. Also, to assess the antibacterial properties of henna crude extract in vitro and compare them with antibiotics. Lawsonia inermis (henna) leaves were extracted with ethanol via using the solvent extraction technique. The pathogens were isolated from wound samples obtained from hospitalized patients in two different hospitals in Duhok city. Culture of thirty isolates had been recognized by routine methods. Different concentrations of ethanol crude extract were acquired and bio-assayed in vitro to inhibit the growth of five human pathogenic Gram-positive bacteria. Agar well diffusion assay was used for achieving henna antibiotic activity. Moreover, antibiotics susceptibility test was done by the disk diffusion method using Muller-Hinton agar medium. The growth of all tested bacteria was suppressed to various degrees by increasing the concentration of the extract. The data has revealed that Staphylococcus aureus was more sensitive than other examined isolates, where the diameter zone of inhibition were ranging from 16-27, 14-25, and 8-18 mm for Staphylococcus epidermidis, Lactobacillus spp. and Streptococcus pneumoniae respectively. The antimicrobial activity of henna extract indicates that it is suitable for being used as significant certain medications. Consequently, henna is active to serve as an anti-bacterial agent against multi-drug resistant Gram-positive bacteria.



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How to Cite
Ibrahim, S., Rasool, C., & Al-Asady, A. (2021). Antimicrobial activity of crude henna extract against Gram-positive bacteria. MicroMedicine, 9(1), 18-26. Retrieved from
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