Extended spectrum and metallo-beta-lactamase Pseudomonas species from poultry and piggery waste

  • Olutayo Israel Falodun Department of Microbiology, University of Ibadan, Nigeria
  • Emmanuel Olugbenga Ikusika Department of Microbiology, University of Ibadan, Nigeria
Keywords: Livestock, Antibiotics resistance, ESBL, MBL, Pseudomonas species


Beta-lactamase producing bacteria have become a public health burden due to antibiotics usage in livestock production. This study was carried out to detect extended spectrum beta-lactamase (ESBL) and metallo-beta-lactamase (MBL) producing Pseudomonas spp. from poultry droppings and piggery dung in Ibadan. Poultry droppings and piggery dung were collected from the University of Ibadan livestock farms while isolation of Pseudomonas spp. was done using Pseudomonas base agar supplemented with Pseudomonas C-N supplement and were conventionally characterized. Detection of ESBL and MBL producing isolates were by double disc synergy test and imipenem-EDTA combined disc test respectively. Antimicrobial susceptibility test was by disc diffusion method against trimethoprim (5 µg), amoxicillin/clavulanate (30 µg), cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), aztreonam (30 µg), imipenem (10 µg), gentamicin (10 µg) and ciprofloxacin (10 µg). A total of 108 Pseudomonas spp. were isolated comprising 53.7% from poultry droppings and 46.3% from piggery dung. The isolates include P. aeruginosa (63.0%), P. putida (24.0%) and P. stutzeri (13.0%). While the ESBL producers were P. aeruginosa (10.2%) and P. stutzeri (1.9%), none of the isolates produced MBL. However, 63.6% the ESBL producers showed resistance to trimethoprim while 61.5% were multidrug resistant. The high prevalence of antibiotics resistance and multidrug resistant strains observed among the Pseudomonas spp. infer that poultry droppings and piggery dung can serves as a reservoir for growth and dissemination of clinically significant antibiotics resistance among bacterial species.

DOI: http://dx.doi.org/10.5281/zenodo.3489572


1. Gosh S, LaPara TM. The effects of sub-therapeutic antibiotic use in farm animals on the proliferation and persistence of antibiotic resistance among soil bacteria. ISME J. 2007; 1: 191-203.

2. Centers for Disease Control and Prevention (CDC). Antibiotics Resistance Threats in the United States. U.S. department of Health and Human Services. Centers for Disease Control and Prevention, 2013.

3. Centers for Disease Control and Prevention (CDC). Antibiotic use in the United States: Progress and Opportunities. National Center for Emerging and Zoonotic Infectious Diseases, 2017.

4. Ayukekbong JA, Ntemgwa M, Atabe AN. The threat of antimicrobial resistance in developing countries: causes and control strategies. Antimicrob Resist Infect Cont. 2017; 6: 47.

5. Kabir J, Umoh VJ, Audu-Okoh E. Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria. Food Cont. 2004; 15: E99-E105.

6. Adelowo OO, Ojo FA, Fagade OE. Prevalence of multiple antibiotic resistance among bacteria isolates from selected poultry waste dumps in Southwestern Nigeria. World J Microbiol Biotech. 2009; 25: E713-E719.

7. Adelowo OO, Fagade OE, Agerso Y. Antibotic resistance and resistance genes I Escherichia coli from poultry farms, southwest Nigeria. J Infect Dev Count. 2014; 8: E1103-E1112.

8. Cavallo JD, Fabre R, Leblanc F, Nicolas-Chanoine MH, Tabaut A. Antibiotic susceptibility and mechanisms of β-lactam resistance in 1310 strains of Pseudomonas aeruginosa: a French multicenter study (1996). J Antimicrob Chemother. 2000; 46: E133-E136.

9. Oliveira KM, Julio PD, Grisolia AB. Antimicrobial susceptibility profile of Pseudomonas spp. isolated from a swine slaughterhouse in Dourados, Mato Grosso do Sul State, Brazil. Argent Microbiol. 2013; 45: E57-E60.

10. Okesola AO, Oni AA, Occurrence of extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa strain in South-West Nigeria. J Med Sci. 2012; 6 (3): E93-E96.

11. Singh I, Jaryal CH, Thakur K, Sood A, Grover SP, Bareja R. Isolation and characterisation of various Pseudomonas species from distinct clinical specimens. J Dent Med Sci. 2015; 14: E80-E84.

12. Clinical and Laboratory Standard Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. Twenty-eighth Information Supplement CLSI document M100-S26. Wayne, P.A. 2018; 38(3): 1-296.

13. Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. J Infect Dis.1988; 10: E867-E878.

14. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012; 18: E268-E281.

15. Yong D, Lee K, Yum JH, Shin HB, Rossolini GM, Chong Y. Imipenem-EDTA disk method for differentiation of metallo-beta-lactamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol. 2002; 40(10): E3798-E3801.

16. Begum S, Salam AM, Alam FK, Begum N, Hassan P, Haq AJ. Detection of extended spectrum β-lactamase in Pseudomonas spp. isolated from two tertiary hospitals in Bangladesh. BioMed Cent Res Not. 2013; 6: 7.

17. Kittinger C, Lipp M, Baumert R, Folli B, Koraimann G, Toplitsch D, et al. Antibiotic resistance patterns of Pseudomonas spp. isolated from the River Danube. Front Microbiol. 2016; 7: 586.

18. Ogefere OH, Agbe O, Ibadin EE. Detection of extended spectrum beta-lactamases among Gram negative bacilli recovered from cattle feaces on Benin City, Nigeria. Notulae Scient Biol. 2017; 9(2): E177-E181.

19. Abd El-Baky RM, Abd El-Azeim NH, Mahmoud-Gad GF. Prevalence of extended-spectrum beta-lactamase, AmpC beta-lactamase, and metallo-beta-lactamase among clinical isolates of Pseudomonas aeruginosa. J Adv Biotechnol Bioeng. 2013; 1: E22-E29.

20. Nepal K, Pnat ND, Neupane B, Belbase A, Baidhja DR, Jha B. Extended spectrum beta-lactamase and metallo beta-lactamase production among Escherichia coli and Klebsiella pneumoniae isolated from different clinical samples in a tertiary health care hospital in Kathmandu, Nepal. Ann Clin Microbiol Antimicrob. 2017; 16: 62.

21. Adelowo OO, Fagade OE. Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turk J Biol. 2012; 35: E101-E145

22. Imanah EO, Beshiru A, Igbinosa EO. Antibiogram profile of Pseudomonas aeruginosa isolated from some selected hospital environmental drains. Asian Pac J Trop Dis. 2017; 7(10): E604-E609.

23. Carissa D, Edward N, Michael A. Extended spectrum beta-lactamase producing Escherichia coli strains of poultry origin in Warri, Nigeria. World J Med Sci. 2013; 8(4): E346-E354.

24. Odumosu BT, Ajetunmobi O, Dada-Adegbola H, Odutayo I. Antibiotic susceptibility pattern and analysis of plasmid profiles of Pseudomonas aeruginosa from human, animal and plant sources. Springer Plus. 2016; 5: 1381.

25. Elhariri M, Hamza D, Elhelw R, Dorgham MS. Extended-spectrum beta-lactamase producing Pseudomonas aeruginosa in Camel in Egypt: potential human hazard. Ann Clin Microbiol Antimicrob. 2017; 16: 21.

26. Kotwal A, Biswas D, Kakati B, Singh M. ESBL and MBL in cefepime resistant Pseudomonas aeruginosa: an update from a rural area in northern India. J Clin Diag Res. 2016; 10(4): E9-E11.

27. Ibrahim DR, Dodd CE, Stekel DJ, Ramsden SJ, Hobman JL. Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm. FEMS Microbiol Ecol. 2016; 92(4): E1-E13.

28. Benie CK, Nathalie G, Adjehi D, Solange A, Fernique KK, Desire K, et al. Prevalence and antimicrobial resistance of Pseudomonas aeruginosa from bovine meat, fresh fish and smoked fish. Arch Clin Microbiol. 2017; 8: 3.

29. Torres JA, Villegas M.V, Quinn JP. Current concepts in antibiotic-resistant Gram-negative bacteria. Exp Rev Anti-infect Ther. 2007; 5: E833-E843.

30. Etebu E, Arikekpar I. Antibiotics: classification and mechanisms of action with emphasis on molecular perspectives. Int J App Microbiol Biotech Res. 2016; 4: E90-E101.

31. Ramana BV, Chaudhury A. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from healthcare associated infections at a tertiary care hospital. J Sci Soc. 2012; 39: E78-E80.

32. Akasaka T, Tanaka M, Yamaguchi A, Sato K. Type II topoisomerase mutations in fluoquinolone-resistant clinical strains of Pseudomonas aeruginosa isolated in 1998 and 1999: role of target enzyme in mechanism of fluoroquinolone resistance. Antimicrob Agents Chemother. 2001; 45: E2263–E2268.

33. Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clin Infect Dis. 2002; 34: 634-640.

34. Lambert PA. Mechanism of antibiotics resistance in Pseudomonas aeruginosa. J Roy Soc Med. 2002; 95(41): 22-26.

35. Olsen I. Biofilm-specific antibiotic tolerance and resistance. Eur J Clin Microbiol Infect Dis. 2015; 34: 877-886.
How to Cite
Falodun, O., & Ikusika, E. (2019). Extended spectrum and metallo-beta-lactamase Pseudomonas species from poultry and piggery waste. MicroMedicine, 7(2), 37-45. Retrieved from http://www.journals.tmkarpinski.com/index.php/mmed/article/view/236
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