Antibacterial activity of methanol extract of Lawsonia inermis against uropathogenic bacteria
Urinary tract infections one of the most common bacterial diseases caused by microbes such as bacteria overcoming the body's defenses in the urinary tract. Extensive studies are needed to identify the bacteria of the infectious agent and to determine the pattern of drug resistance and the identification of effective drugs for proper treatment. Therefore, the purpose of this study was to determine the antibiotic resistance pattern of urinary pathogens and compare it with methanol extract of Lawsonia inermis. Urine samples were collected using the mid-stream "clean catch" technique and examined bacteriologically using standard procedures. The antibiotic resistance pattern of each uropathogen isolated was carried by Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Methanol extract of the plant was prepared by maceration method and its inhibitory effect on uropathogen isolates studied using well diffusion assay. Based on obtained results, the most common uropathogen isolated was Escherichia coli and the most frequent uropathogens were Gram negative rod bacteria. Most resistant antibiotics were Cefazolin, Ampicillin, Vancomycin and Nitrofurantoin. Methanol extract of Lawsonia inermis showed good antibacterial potential against all uropathogen bacterial isolates. The results suggest that the Lawsonia inermis possess antibacterial properties that support the folk medicinal use of this plant.
2. Mishra MP, Rath S, Swain SS, Ghosh G, Das D, Padhy RN. In vitro antibacterial activity of crude extracts of 9 selected medicinal plants against UTI causing MDR bacteria. J King Saud Univ Sci. 2017; 29(1): 84-95.
3. Borade AS, Kale BN, Shete RV. A phytopharmacological review on Lawsonia inermis (Linn.). Int J Pharm Life Sci. 2011; 2(1): 536-534.
4. Habbal O, Hasson S, El-Hag A, Al-Mahrooqi Z, Al-Hashmi N, Al-Bimani Z, et al. Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa. Asian Pacific J Trop Biomed. 2011; 1(3): 173-176.
5. Beyene G, Tsegaye W. Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in jimma university specialized hospital, southwest Ethiopia. Ethiopian J Health Sci. 2011; 21(2): 141-146.
6. Sahoo S, Rout B, Mekap S, Dhal N. Antimicrobial activity of Acalypha godseffiana against selected urinary tract infection causing pathogens. J Pharmacogn Phytochem. 2014; 3(4): 83-85.
7. Bonadio M, Meini M, Spitaleri P, Gigli C. Current microbiological and clinical aspects of urinary tract infections. Eur Urol. 2001; 40(4): 439-445.
8. Shakiba M, Kariminik A, Parsia P. Antimicrobial activity of different parts of Phoenix dactylifera. Int J Mol Clin Microbiol. 2011; 1: 107-111.
9. Shahidi Bonjar G. Screening for antibacterial properties of some Iranian plants against two strains of Escherichia coli. Asian J Plant Sci. 2004; 3(3): 310-314.
10. Valgas C, Souza SMd, Smânia EF, Smânia Jr A. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol. 2007; 38(2): 369-380.
11. Nalubega R, Kabasa JD, Olila D, Kateregga J. Evaluation of antibacterial activity of selected ethnomedicinal plants for poultry in Masaka district, Uganda. Res J Pharmacol. 2011; 5(2): 21-18.
12. Shahidi Bonjar G, Kariminik A, Heidari M, Ghasemzadeh M, RashidFarrokhi P, Moein M, et al. Anti-Pseudomona and antibacilli activity of some medicinal plants of Iran. Daru. 2003; 11(4): 157-163.
13. Shahidi Bonjar GH, Aghighi S, Karimi Nik A. Antibacterial and antifungal survey in plants used in indigenous herbal-medicine of south east regions of Iran. J Biol Sci. 2004; 4(3): 405-412.
14. Bollenbach T. Antimicrobial interactions: mechanisms and implications for drug discovery and resistance evolution. Curr Opin Microbiol. 2015; 27: 1-9.
15. Joshi B, Sah GP, Basnet BB, Bhatt MR, Sharma D, Subedi K, et al. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). J Microbiol Antimicrob. 2011; 3(1): 1-7.
16. Foxman B. The epidemiology of urinary tract infection. Nature Rev Urol. 2010; 7(12): 653.
17. Dielubanza EJ, Schaeffer AJ. Urinary tract infections in women. Med Clin. 2011; 95(1): 27-41.
18. Cheesman MJ, Ilanko A, Blonk B, Cock IE. Developing new antimicrobial therapies: Are synergistic combinations of plant extracts/compounds with conventional antibiotics the solution? Pharmacogn Rev. 2017; 11(22): 57.
19. Chaudhary G, Goyal S, Poonia P. Lawsonia inermis Linnaeus: a phytopharmacological review. Int J Pharmac Sci Drug Res. 2010; 2(2): 91-98.
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