In vitro anthelmintic activity of Allium sativum, Ferula asafoetida, Syzygium aromaticum and their active components against Fasciola gigantica

Pradeep Kumar, Dinesh Kumar Singh


The effect of dried clove powder of Allium sativum, dried latex powder of Ferula asafoetida and dried flower bud powder of Syzygium aromaticum in in vitro treatment against liver fluke Fasciola gigantica were studied. The anthelmintic activities of all the three plants were time and concentration dependent. A. sativum bulb powder 8h LC50 against F. gigantica was 5.48 mg/ml. Among different organic extracts, ethanol extract was more toxic than other organic extract. The ethanol extract of S. aromaticum (2h LC50 2.95 mg/ml) was more toxic against F. gigantica than A. sativum (2h LC50 3.48 mg/ml) and F. asafoetida (2h LC50 3.94 mg/ml). The 8h LC50 of column purified fraction of dried flower bud powder   of S. aromaticum was 1.02 mg/ml whereas dried clove powder of A. sativum and latex powder of F. asafoetida were 1.87 and 2.75 mg/ml, respectively. The product of A. sativum, F. asafoetida and S. aromaticum may be used as anthelmintic.


Fasciola gigantica; Allium sativum; Ferula asafoetida; Syzygium aromaticum; Anthelmintic activity

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