2024-03-28T15:43:36Z
http://www.journals.tmkarpinski.com/index.php/index/oai
oai:ojs2.journals.tmkarpinski.com:article/2
2021-02-26T10:51:27Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180331 2018 eng "
2449-8955
dc
Petrified Geobacillus thermoglucosidasius colony to strontianite
Yoshida, Naoto
Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan
Murai, Rie
Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan
Kiyoshi, Keiji
Department of Biochemistry and Applied Biosciences, University of Miyazaki, 1-1 Gakuen Kibanadai-Nishi, Miyazaki 889-2192, Japan
When biomass of the thermophilic bacteria Geobacillus thermoglucosidasius is brought into contact with a hydrogel containing sodium acetate and strontium, the biomass petrifies and hardens, becoming a mineralized thin film after incubation at 60˚C for 72 h. Analysis by energy dispersive X-ray and X-ray diffraction shows that the mineralized thin film is strontianite. This is the first report of biomass completely changing to strontianite. Strontianite of thermophilic bacterial origin may be formed in the hydrothermal oligotrophic environment of the deep subsurface.
DOI: http://dx.doi.org/10.5281/zenodo.1146702
JBródka Publisher
2018-03-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/2
European Journal of Biological Research; Vol 8 No 1 (2018): January-March 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/3
2021-02-26T10:51:27Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180331 2018 eng "
2449-8955
dc
Effects of cobalt and manganese on biomass and nitrogen fixation yields of a free-living nitrogen fixer - Azotobacter chroococcum
Orji, Justina
Federal University of Technology Owerri, Department of Microbiology, P.M.B 1526 Owerri, Nigeria
Ngumah, Chima
Federal University of Technology Owerri, Department of Microbiology, P.M.B 1526 Owerri, Nigeria
Asor, Hanna
Federal University of Technology Owerri, Department of Microbiology, P.M.B 1526 Owerri, Nigeria
Anuonyemere, Anulika
Federal University of Technology Owerri, Department of Microbiology, P.M.B 1526 Owerri, Nigeria
The effects of different concentrations of cobalt and manganese on the biomass and the ability of Azotobacter chroococcum to fix nitrogen were investigated. In vitro trials were conducted in Jensen’s (nitrogen free) broth (half strength) under continuous air flow, incubated at ambient room temperatures for seven days. Results obtained showed that 12.5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l, and 200 mg/l concentrations of cobalt and manganese respectively enhanced microbial growth of Azotobacter chroococcum concomitantly. However, nitrogen fixation was enhanced only at 12.5 mg/l and 25 mg/l concentrations for cobalt, and only at 12.5 mg/l concentration for manganese. Statistical analysis revealed no significant difference in the specific growth rates and nitrogen fixations respectively, between the cobalt and manganese trials. Kinetic modeling revealed that nitrogen fixation was associated with biomass concentration, and not with cell mass growth.
DOI: http://dx.doi.org/10.5281/zenodo.1157098
JBródka Publisher
2018-03-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/3
European Journal of Biological Research; Vol 8 No 1 (2018): January-March 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/4
2021-02-26T10:51:28Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180214 2018 eng "
2449-8955
dc
Neurospora tetraspora D. Garcia, Stchigel & Guarro (= Gelasinospora tetrasperma Dowding) as a first record to Egypt
Moubasher, A. H.
Department of Botany and Microbiology, Faculty of Science, Assiut Universiy, P.O. Box 71526, Assiut, Egypt; Assiut University Mycological Centre, Assiut University, P.O. Box 71526, Assiut, Egypt
Abdel-Sater, M. A.
Department of Botany and Microbiology, Faculty of Science, Assiut Universiy, P.O. Box 71526, Assiut, Egypt; Assiut University Mycological Centre, Assiut University, P.O. Box 71526, Assiut, Egypt
Soliman, Zeinab
Assiut University Mycological Centre, Assiut University, P.O. Box 71526, Assiut, Egypt
An interesting isolate of homothallic, ascosporic filamentous fungus having 4-spored asci, was recovered once from a non-rhizosphere soil sample collected from a grapevine plantation in the village of El-Khawaled, Sahel-Saleem city, Assiut. It was isolated on DYM agar plate at 25°C in June 2008. The isolate was identified phenotypically and genotypically as Neurospora tetraspora (= Gelasinospora tetrasperma) and was deposited in the culture collection of Assiut University Mycological Centre as AUMC no. 6784 and ITS gene sequence of the strain was deposited at the National Center for Biotechnology Information (NCBI) and assigned a GenBank accession number JQ425383. N. tetraspora is being recorded in the current work for the first time in Egypt. By this addition, the genus is now known in Egypt by four species. A key is provided for the four species.
DOI: http://dx.doi.org/10.5281/zenodo.1165603
JBródka Publisher
2018-03-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/4
European Journal of Biological Research; Vol 8 No 1 (2018): January-March 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/6
2021-02-26T10:51:26Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180331 2018 eng "
2449-8955
dc
Antioxidant potential of the farmer preferred selections of Solanum aethiopicum vegetable consumed in central Uganda
Sekulya, S.
Department of Biochemistry, College of Natural Sciences, Makerere University, Uganda
Nandutu, A.
Department of Biochemistry, College of Natural Sciences, Makerere University, Uganda
Namutebi, A.
Department of Food Technology and Nutrition, College of Agricultural and Environmental Sciences, Makerere University, Uganda
Ssozi, J.
Department of Food Technology and Nutrition, College of Agricultural and Environmental Sciences, Makerere University, Uganda
Masanza, M.
Department of Agricultural and Biological Sciences, Faculty of Science and Technology, Uganda Christian University, Uganda
Kabod, B.
Department of Agricultural and Biological Sciences, Faculty of Science and Technology, Uganda Christian University, Uganda
Jagwe, J. N.
Farmgain Africa Limited, Plot 1 Kimera Road, 2nd Floor Ntinda Shopping Mall P.O. Box 21717 Kampala, Uganda
Kasharu, A.
Coalition for Health Agricultural Income Networks, Plot 115, Busega, Kampala - Masaka, Uganda
Rees, D.
Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Chatham, Kent ME4 4TB, UK
Kizito, E. B.
Department of Agricultural and Biological Sciences, Faculty of Science and Technology, Uganda Christian University, Uganda
In addition to the rich micronutrient value, indigenous vegetables are regarded as possessing medicinal attributes. The Solanaceae family has over 1000 species worldwide, with a number of indigenous species originating in Africa. The most popular leafy vegetable in Uganda is the Solanum aethiopicum (Nakati). The objective of this study was to determine the selected phytochemical attributes, chlorophyll content, moisture content and total antioxidant activity of the farmer preferred selections within the landraces of Solanum aethiopicum leafy vegetable in Uganda. The antioxidant activity was achieved by screening the leaf extracts for their free radical scavenging properties using diphenyl picryl hydrazyl (DPPH) and ascorbic acid as standard. The ability of the extracts to scavenge DPPH radical was determined spectrophometrically at 517nm.The study showed that all the landraces had a high polyphenol and flavonoid content with SAS185/P/2015 containing the highest flavonoid content (3.16±0.06 mg QE/g fw). SAS1641/2015 showed the highest total polyphenol content of 7.79±0.27 mg GAE/g fw and also showed the highest vitamin C content. This contributed to the high total antioxidant activity of 2.79±0.01 and 5.43±0.02 mg AAE/g fw when using FRAP and DPPH methods respectively. SAS145/2015 presented the highest chlorophyll content of 19.69±0.01 mg/g dwb. All the landraces showed a high percentage moisture content that ranged from 82.66±0.35 to 84.21±0.48%. These results are of nutraceutical significance and hence confirm their usage as medicinal vegetables.
DOI: http://dx.doi.org/10.5281/zenodo.1195552
JBródka Publisher
2018-03-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/6
European Journal of Biological Research; Vol 8 No 1 (2018): January-March 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/12
2021-02-26T10:51:26Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180331 2018 eng "
2449-8955
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Comparative stem anatomy of four taxa of Calycanthaceae Lindl.
Paudel, Niroj
Division of Biological Resource Sciences, Kangwon National University, Chuncheon, 24341, South Korea
Heo, Kweon
Division of Biological Resource Sciences, Kangwon National University, Chuncheon, 24341, South Korea
The anatomical character is potential value in Calycanthaceae for their taxonomic study. Four species of Calycanthaceae were collected for this experiment. The experiment was done using the resin methods for preparation of the permanent slide for anatomical studies. The anatomical character like two traces of the unilocular vascular bundle, in the primary vascular cylinder, contains four cortical vascular bundles in the stem, the unilocular structure of primary cylinder, the presence of numerous intercellular space in phloem, the presence of oil cell in the form of scatter in Calycanthus whereas small size in Chimonathus. Calycanthus possess boarder pit with circular aperture while Chimonanthus possess elliptical. The tracheid is a characteristic feature of the spiral band wider in Chimonanthus than that of Calycanthus and Sinocalycanthus. The noted sclerenchymatous cells are grouped of the colony which is a characteristic feature of Sinocalycanthus and Calycanthus but in case of Chimonanthus is the long chain with the layer of the cell. Collenchymatous cell was circular with an intercellular in Calycanthus; ovoid shape with the intercellular in Chimonanthus but in Sinocalycanthus is elongation with the minor regular shape. The different character of pith cells found in hexagonal and circular shape which is also distinguished feature in Calycanthaceae. The valu-able stem anatomical characters are the importance of their function, ontogeny, and phylogeny.
DOI: http://dx.doi.org/10.5281/zenodo.1199578
JBródka Publisher
2018-03-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/12
European Journal of Biological Research; Vol 8 No 1 (2018): January-March 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/13
2021-02-26T10:51:27Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180330 2018 eng "
2449-8955
dc
Diversity of inulinase-producing fungi associated with two Asteraceous plants, Pulicaria crispa (Forssk.) and Pluchea dioscoridis (L.) growing in an extreme arid environment
Khalil, Doaa M. A.
Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt
Massoud, Mohamed S.
Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt
Abdelrahman, Mostafa
Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt; Graduate School of Life Sciences, Tohoku University, Sendai 9808577, Japan
El-Zayat, Soad A.
Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt
El-Sayed, Magdi A.
Botany Department, Faculty of Sciences, Aswan University, Aswan 81528, Egypt; Unit of Environmental Studies and Development, Aswan University, Aswan 81528, Egypt
Inulinases are potentially valuable enzymes catalyze the hydrolysis of plant’s inulin into high fructose syrups as sweetening ingredients for food industry and ethanol production. The high demands for inulinase enzymes have promoted interest in microbial inulinases as the most suitable approach for biosynthesis of fructose syrups from inulin. Arid land ecosystem represents a valuable bioresource for soil microbial diversity with unique biochemical and physiological properties. In the present study, we explored the fungi diversity associated with the rhizosphere and rhizoplane of two desert medicinal plants namely Pluchea dioscoridis and Pulicaria crispa growing in the South-Eastern desert of Aswan, Egypt. A total of 180 fungal isolates were screened based on their ability to grow on potato dextrose agar medium supplemented with 1% inulin. The isolated fungal colonies were morphologically identified according to cultural characteristics and spore-bearing structure. In addition, the inulinase activity of the isolated fungi was examined spectrophotometrically. Among these, Aspergillus terreus var. terreus 233, Botrytis cinerea, Aspergillus aegyptiacus, Cochliobolus australiensis 447 and Cochliobolus australiensis exhibited high inulinase activity ranging from 5.05 to 7.26 U/ml. This study provides a promising source of microbial inulinase, which can be scaled up for industrial applications.
DOI: http://dx.doi.org/10.5281/zenodo.1205649
JBródka Publisher
2018-06-22 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/13
European Journal of Biological Research; Vol 8 No 2 (2018): April-June 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/17
2021-02-26T10:51:28Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180322 2018 eng "
2449-8955
dc
Optimization of kojic acid production conditions from cane molasses using Plackett-Burman design
Zohri, Abdel-Naser A.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Mahmoud, Ghada Abd-Elmonsef
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Saddek, Nermien H.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt; Medical & Applied Science College in Jubail, Imam Abdulrahman Bin Faisal - University, Dammam, Saudi Arabia
Hanafy, Radwa Adel
Sugar Technology Research Institutes, Assiut University, Assiut, Egypt
Fungal synthesis of kojic acid has gained more interest in these days as an alternative way to chemical synthetic. The aspect of the microbial fermentation process is to develop a suitable culture medium to obtain the maximum amount of kojic acid using statistical methods. In this study; different selected three isolates of Aspergillus flavus (No 1, 2 and 3) were screened for their ability to produced kojic acid and the isolate No 3 was the highest kojic acid producer one. The capability of A. flavus No 3 to produce kojic acid was improved using Plackett-Burman design. From ten different agro-industrial wastes cane molasses recorded the highest kojic acid productivity with 2.24 g/l-1 day-1 and was the most effective parameter plays a crucial role in Plackett-Burman design. Maximum kojic acid production (24.65 g/l) by A. flavus (No. 3) obtained under the fermentation conditions: incubation temperature at 25oC, incubation time 9 days, pH 3, inoculum size 0.5%, shaking rate at 150 rpm and medium constituents: Cane molasses 60 g/l, yeast extract 7 g/l, KH2PO4 2 g/l, ZnSO4·7H2O 100 µg/l and MgSO4·7H2O 1 g/l with regression analysis (R2) 99.45% and 2.33-fold increase in comparison to the production of the original level (10.6 g/l).
DOI: http://dx.doi.org/10.5281/zenodo.1211517
JBródka Publisher
2018-06-22 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/17
European Journal of Biological Research; Vol 8 No 2 (2018): April-June 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/20
2021-02-26T10:51:32Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
dc
Comparison of biofilm-producing Enterococcus faecalis, Enterococcus faecium, and unusual Enterococcus strains
Sieńko, Anna
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
Ojdana, Dominika
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
Majewski, Piotr
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
Sacha, Paweł
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
Wieczorek, Piotr
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
Tryniszewska, Elżbieta
Department of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bialystok, 15a Waszyngtona Street, 15-269 Bialystok, Poland
The present study focused on determining the prevalence of biofilm-forming ability in Enterococ-cus faecalis, E. faecium, and unusual Enterococcus clinical isolates, and comparison of resistance and the prevalence of selected virulence factors among biofilm-positive strains. The ability to form biofilm was detected in 13.3% of E. faecalis, 90% of E. faecium, and 57.1% of unusual Enterococcus strains (p=0.026). All E. faecalis strains were susceptible to β-lactams, while 37.5% of unusual and all E. faecium isolates were resistant to these antibiotics. Resistance to gentamicin was detected in 75% of E. faecalis, 55.5% of E. faecium, and 25% of other strains; resistance to streptomycin in 25%, 83.3%, and 50%, respectively. Analysis of the virulence revealed that the enterococcal surface protein (esp) gene was found in all E. faecium, 75.0% of E. faecalis, and 37.5% of other strains; collagen adhesin gene (ace) in 100%, 25.0%, and 37.5%; and hyaluronidase gene (hyl) in 83.3%, 0%, and 37.5%, respectively. Analysis of the resistance and virulence patterns showed that E. faecium isolates had the greatest variety of virulence and resistance determinants, while the lowest variety was exhibited by unusual strains. These findings indicate that unusual biofilm-producing Enterococcus strains have lower resistance and virulence potency than E. faecalis and E. faecium.
DOI: http://dx.doi.org/10.5281/zenodo.1000837
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/20
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/23
2021-02-26T10:51:31Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
dc
Statistical optimization as a powerful tool for indole acetic acid production by Fusarium oxysporum
Mahmoud, Ghada Abd-Elmonsef
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Mostafa, Hassan H. A.
Central Laboratory of Organic Agriculture, Agricultural Research Center, Giza 12619, Egypt
Crop production is challenged in our world by increasing food demands, decrease natural resource bases and climatic change. Nowadays plant growth regulators works like fertilizers in increasing plant growth production efficiency and needed to produce in large industrial scale. Fermentation condition and medium constituents can significantly affect on the product production and designing an acceptable fermentation medium is critical importance. In this paper Fusarium sp. could be considered as promising indole-3-acetic acid producers with the ability to improve the production using statistical methods. The results showed that fermentation type, incubation temperature and L-tryptophan were the most influencing parameters on the production. Maximum IAA production by Fusarium oxysporum was 300.4 mg/l obtained under the fermentation conditions: temperature at 25oC, incubation period 5 days, pH 7, inoculums size 2%, shaking rate at 150 rpm and medium constituents: Glucose 40 g/l, yeast extract 3 g/l, L-tryptophan 1 g/l, KH2PO4 2 g/l, NaNO3 4 g/l, MgSO4·7H2O 0.1 g/l with regression analysis (R2) 99.67% and 2.12-fold increase in comparison to the production of the original level (142 mg/l).
DOI: http://dx.doi.org/10.5281/zenodo.1012348
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/23
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/26
2021-02-26T10:51:25Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180605 2018 eng "
2449-8955
dc
Effect of selenium on nutritive value of purslane (Portulaca oleracea L.)
Gamal, Khedr F.
Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Salama, Hoda Mohamed
Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Ismaiel, Shimaa A.
Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
Purslane (Portulaca oleracea) one of the auxiliary plants was traditionally consumed in many parts of the world for its nutritional and medicinal benefits. The nutrient components of purslane such as total protein, total carbohydrates and mineral content such as macro elements (Na, K, Ca and Mg) and micro elements (Fe, Cu, Pb and Zn) were estimated at different concentrations of selenium which treated in soil where the plant cultivated. The protein and carbohydrate contents of leaves as well as protein of stems increase with increasing the selenium concentration, while protein and carbohydrate of roots as well as carbohydrate of stems decrease with increasing Se concentration. The mineral content was also affected by Se concentration, Fe, Cu and Zn of leaves decreased with increasing Se concentration, while K, Ca, Mg and Na are directly proportional with Se concentration. In stems, Zn only is inversely proportional with Se concentration. In roots, Fe, Cu, Mg and K are inversely proportional with Se concentration, while Na, Ca and Zn are directly proportional. The findings of this study revealed that carbohydrates, protein and mineral contents of purslane can be affected and controlled by selenium concentration.
DOI: http://dx.doi.org/10.5281/zenodo.1283418
JBródka Publisher
2018-06-22 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/26
European Journal of Biological Research; Vol 8 No 2 (2018): April-June 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/30
2021-02-26T10:51:25Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180606 2018 eng "
2449-8955
dc
Histomorphological responses to aqueous crude leaf extract of Alafia barteri on prefrontal cortex, heart, kidney, liver and testis of adult male Sprague-Dawley rats
Aderemi, Adelakun Sunday
Department of Human Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Nigeria
Ogunlade, Babatunde
Department of Human Anatomy, School of Health and Health Technology, Federal University of Technology, Akure, Ondo State, Nigeria
Omotoso, Olunsegun Dare
Department of Anatomy, Faculty of Basic Medical Sciences, Kogi State University Anyigba, Kogi State, Nigeria
Phytonutrients present in Alafia barteri leaves include antioxidants which serves to protect cells and tissues against detrimental effects of reactive oxygen species and other free radicals. This research work was targeted at investigating the activities of oral administration of aqueous leaf extract of Alafia barteri on the histology of the prefrontal cortex, heart, kidney, liver and testis of adult Sprague Dawley rats. Twelve (n=12) adult male Sprague Dawley rats weighing between 170-200 g (4-6 weeks old) were used for this study; they were divided into 2 groups of six rats each. The control group A received 2 ml/kg normal saline and treated group B received 500 mg/kg body weight aqueous extract of Alafia barteri for twenty eight days. The gross anatomical parameters of the selected organs and their histology were assessed. The gross anatomical and histological observation of the prefrontal cortex, heart, kidney, liver and testis revealed no visible distortion in Alafia barteri extract treated group when compared with control. Aqueous leaf extract of Alafia barteri thus has no deleterious effects on the histological profile of the prefrontal cortex, heart, kidney, liver and testis of the rats.
DOI: http://dx.doi.org/10.5281/zenodo.1284554
JBródka Publisher
2018-06-22 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/30
European Journal of Biological Research; Vol 8 No 2 (2018): April-June 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/31
2021-02-26T10:51:25Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180521 2018 eng "
2449-8955
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Pectin coating of titanium and polystyrene surfaces modulates the macrophage inflammatory response
Mieszkowska, Anna
Environmental Biotechnology Department, Faculty of Energy and Environmental Engineering, Silesian University of Technology, 44-100 Gliwice, Poland
Folkert, Justyna
Environmental Biotechnology Department, Faculty of Energy and Environmental Engineering, Silesian University of Technology, 44-100 Gliwice, Poland
Burke, Bernard
Faculty of Health and Life Sciences, University of Coventry, CV1 2DS Coventry, UK
Addison, Owen
Institute of Clinical Sciences, School of Dentistry, University of Birmingham, Birmingham, B5 7EG, UK; Faculty of Medicine and Dentistry, School of Dentistry, University of Alberta, Edmonton, T6G 1C9, AB, Canada
Gurzawska, Katarzyna
Institute of Clinical Sciences, School of Dentistry, University of Birmingham, Birmingham, B5 7EG, UK
Titanium has been used with success for bone anchoring of dental implants. However, when implant surfaces are exposed to the oral environment, the progression of peri-implantitis triggered by specific oral bacteria has been reported. Bacterial colonization of implants leads to prolonged immune cell activation and bone resorption. A new strategy to improve implant biocompatibility and prevent peri-implantitis is to develop pectin surface nanocoatings. These plant-derived polysaccharides are promising candidates for surface nanocoatings of titanium implants due to their osteogenic and anti-inflammatory properties. Therefore, the aim of the study was to evaluate the in vitro effect of nanocoating with plant-derived rhamnogalacturonan-I (RG-I) on pro- and anti-inflammatory responses of primary human monocyte-derived macrophages (HMDMs) induced by Escherichia coli LPS and Porphyromonas gingivalis bacteria. In the present study, two different types of surface materials, tissue culture polystyrene (TCPS) plates and titanium (Ti) discs, coated with pectic polysaccharides, potato unmodified RG-I (PU) and potato dearabinanated RG-I (PA), have been examined. The inflammatory responses of HMDMs after E. coli LPS/P. gingivalis stimulation were investigated through gene expression measurements of pro- and anti-inflammatory cytokines. The results showed that PU and PA decreased expression of the proinflammatory genes tumour necrosis factor-alpha (TNFA), interleukin-1 beta (IL1B) and interleukin-8 (IL8) in activated HMDMs cultured on TCPS/Ti surfaces. In contrast, the effects on anti-inflammatory interleukin-10 (IL10) gene expression were not significant. The results indicate that RG-Is should be considered as a candidate for organic nanocoatings of titanium implant surfaces in order to limit host proinflammatory responses and improve bone healing.
DOI: http://dx.doi.org/10.5281/zenodo.1250541
JBródka Publisher
2018-06-22 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/31
European Journal of Biological Research; Vol 8 No 2 (2018): April-June 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/32
2021-02-26T10:51:23Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180718 2018 eng "
2449-8955
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Independent distribution of blood group types and two genetically determined traits in a female population
Singh, Arvind Kumar
Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India
Yangchen, Palmo
Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India
Certain traits in humans are known to be neutral in nature as they do not influence fitness of the individuals. Traits like ABO blood group, phenylthiocarbamide (PTC) tasting and ear lobe structure are genetically determined and follow Mendelian pattern of inheritance. Genes deciding their expression are situated on separate chromosomes and therefore would be certainly following independent assortment during gametogenesis. Data regarding association of these traits were collected from human female subjects to test whether blood group types show their dependency with other two features. An analysis in this regard clearly indicated that there exist no association between blood group type and PTC tasting and also between blood group and ear lobe structure.
DOI: http://dx.doi.org/10.5281/zenodo.1314731
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/32
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/41
2021-02-26T10:51:24Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180707 2018 eng "
2449-8955
dc
Profile of major and emerging mycotoxins in sesame and soybean grains in the Federal Capital Territory, Abuja, Nigeria
Fapohunda, Stephen O.
Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria
Anjorin, Toba S.
Department of Crop Protection, Faculty of Agriculture, University of Abuja, PMB117, Abuja, Nigeria
Sulyok, Michael
Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr, 20, A-3430 Tulln, Austria
Krska, Rudolf
Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr, 20, A-3430 Tulln, Austria
The spectrum of major and emerging mycotoxins in sesame and soybean grains from the six zones of the Federal Capital Territory (FCT), Abuja, Nigeria was determined using Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS). A total of 47 samples (24 sesame and 23 soybean were collected from farmers’ stores. Seven regulated mycotoxins in sesame and five in soybean including aflatoxin B1 (AFB1), aflatoxin B2 (AFB2) and fumonisin B1 (FB1) were detected. However, concentrations were generally lower than regulatory limits set in the EU for raw grains with the exception of ochratoxin A (OTA) exhibiting a maximum concentration level of 23.1 µg kg-1 in one of the soybean samples. This is the first report concerning the contamination of sesame and soybean in Abuja, FCT-Nigeria with the emerging mycotoxins addressed by recent European Food Safety Authority (EFSA) opinion papers totalling 10 in number. These include beauvericin (BEA), moniliformin (MON), sterigmatocystin (STE), altertoxin-I (ATX-I), alternariol (AOH), alternariol methylether (AME) though at relatively low µg kg-1 range. This preliminary data indicate that sesame and soybean might be relatively safe commodities in view of the profile of mycotoxins.
DOI: http://dx.doi.org/10.5281/zenodo.1307184
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/41
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/54
2021-02-26T10:51:23Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180718 2018 eng "
2449-8955
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Incidence of community acquired ESBL-producing bacteria among asymptomatic University students in Anambra State, Nigeria
Chukwunwejim, Chidimma R.
Department of Pharmaceutical Microbiology and Biotechnology, Enugu State University of Science and Technology, Enugu, Nigeria
Eze, Peter M.
Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka
Ujam, Nonye T.
Department of Pharmaceutical Microbiology and Biotechnology, Enugu State University of Science and Technology, Enugu, Nigeria
Abonyi, Isaiah C.
Department of Environmental Health Science, Nnamdi Azikiwe University, Awka, Nigeria
Ejikeugwu, Chika P.
Department of Applied Microbiology, Ebonyi State University, Abakiliki, Nigeria
Abony, Dominic O.
Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria
Esimone, Charles O.
Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria
This study was conducted to investigate the incidence of community acquired extended-spectrum β-lactamase (ESBL)-producing bacteria among asymptomatic students of Nnamdi Azikiwe University, Awka, Anambra State, South-East Nigeria. A total of 102 non-duplicate strains of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were isolated from fecal samples (n=273) collected from the participating students. The isolates were subjected to antimicrobial susceptibility tests to determine their antimicrobial resistance profile. Their multiple antibiotic resistance (MAR) indices were also evaluated. Screening of the isolates for possible ESBL production was carried out by disk diffusion test using cefotaxime and ceftazidime disks. ESBL-production by the resistant strains was confirmed using the double-disk synergy test. Most of the isolates were found to be multi-drug resistant, as all K. pneumoniae and P. aeruginosa strains (100%), and 98.4% of the E. coli strains, had MAR indices ≥0.2. A total of 22 ESBL-producing bacterial species were confirmed, and the frequency of E. coli, K. pneumoniae and P. aeruginosa isolates among the ESBL-producing bacteria were n=20 (90.9%), n=2 (9.1%), and n=0 (0.0%) respectively. The total number of ESBL-producing bacterial strains isolated accounted for 8.1 % of the entire sample population. Although this prevalence rate may not indicate an alarming situation, it is important that the proliferation of ESBL-producing bacteria in the community be contained, since a high incidence of ESBL-producing organisms will create significant therapeutic problems in the near future. There is therefore need to develop strategies to reduce their spread in the community especially through monitoring, surveillance and proper detection protocol.
DOI: http://dx.doi.org/10.5281/zenodo.1314719
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/54
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/55
2021-02-26T10:51:22Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180910 2018 eng "
2449-8955
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In vitro studies of iron absorption and activity of glutathione peroxidase in intestinal mucosa of the chicken
Markovs, Jurijs
Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
Galuza, Agate
Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
Basova, Natalija
Institute of Biology of the University of Latvia, Riga, Latvia
Knipse, Gundega
Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
Vasiljeva, Svetlana
Institute of Biology of the University of Latvia, Riga, Latvia
Smirnova, Galina
Institute of Biology of the University of Latvia, Riga, Latvia
We examined the absorption of iron, the activity of selenoprotein glutathione peroxidase (GSH·Px) and cellular compartmentalization of metal in the chicken duodenum and ileum. The method of accumulating mucosa preparation (AMP) was used. It was shown that the intestinal iron accumulation is dose-dependent process, which has two components: transcellular and paracellular. The realization of these pathways is region-specific and depends on exposed iron levels. Slightly elevated iron status of intestinal mucosa does not influence activity of GSH·Px. At the same time the results indicate that the activity of glutathione peroxidase can be altered by iron overload. Immunohistochemistry revealed that stainable iron could be co-localized to the endolysosomal compartment. How the activity of enzyme can be affected by oxidative stress and competitive interactions of iron with selenium are discussed.
DOI: http://dx.doi.org/10.5281/zenodo.1412784
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/55
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/57
2021-02-26T10:51:22Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180828 2018 eng "
2449-8955
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Biologically active phenolic acids produced by Aspergillus sp., an endophyte of Moringa oleifera
Abonyi, Dominic O.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
Eze, Peter M.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
Abba, Chika C.
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
Ujam, Nonye T.
Department of Pharmaceutical Microbiology and Biotechnology, Enugu State University of Science and Technology, Enugu, Nigeria
Proksch, Peter
Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University, Düsseldorf, Germany
Okoye, Festus B. C.
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
Esimone, Charles O.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria
This study investigates the secondary metabolites of an endophytic Aspergillus sp. isolated from leaves of M. oleifera growing in Anambra State, South-Eastern Nigeria. Antimicrobial and antioxidant screening of the fungal extract and isolated compounds, as well as cytotoxicity assay of the extract against cisplatin-sensitive A2780 (sens) and cisplatin-resistant A2780 (cisR) ovarian cancer cell lines were carried out using standard methods. Chemical investigations of the fungal extract involving a combination of different chromato-graphic methods and spectroscopic techniques were carried out to isolate and characterize the constituents of the extract. At a concentration range of 1-4 mg/ml, the crude extract of Aspergillus sp. showed mild antimicrobial activity against Bacillus subtilis, Klebsiella pneumoniae, and Candida albicans. The fungal extract showed good antioxidant activity at 500 µg/ml, with an inhibition of 72.1%. Also, at 100 µg/ml, the extract showed excellent cytotoxic activity against A2780 (sens) and A2780 (cisR), with growth inhibitions of 105.1% and 105.5% respectively. Two known pharmacologically active phenolic compounds (p-hydroxyphenyl acetic acid and ferulic acid) were isolated from the fermentation extract of the endophytic fungus. At 250 µg/ml, ferulic acid exhibited an excellent antioxidant activity with an inhibition of 90.4%, while an inhibition of 35.4% was recorded for p-hydroxyphenyl acetic acid. Ferulic acid also showed a mild antifungal activity at 500 µg/ml against A. niger with an IZD of 2 mm. p-Hydroxyphenyl acetic acid showed no antimicrobial activity. These results further confirm the potentials of endophytic fungi associated with Nigerian plants as source of bioactive compounds with pharmaceutical or industrial applications.
DOI: http://dx.doi.org/10.5281/zenodo.1404981
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/57
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/68
2021-02-26T10:51:23Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180816 2018 eng "
2449-8955
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Achillea millefolium L. subsp. millefolium essential oil’s antifungal effect
Aydın, Sinem
Giresun University, Department of Biology, Faculty of Science and Arts, Giresun, Turkey
Sevindik, Emre
Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
This study was carried out with the aim of determining the antifungal effect of the essential oil isolated from Achillea millefolium subsp. millefolium plant against pathogenic fungi. In order to test the antifungal effect of the oil, an analysis was conducted on a total of 4 pathogen fungi which included Candida albicans, Candida tropicalis, Candida parapsilosis and Saccharomyces cerevisiae, and the effect of the essential oil on the growth of these fungi was investigated. The essential oil of A. millefolium ssp. millefolium had varying degrees of effect on the tested fungi. The highest antifungal effect was found against S. cerevisiae; whereas the lowest antifungal effect was found against C. parapsilosis. Nystatin showed a higher activity than the essential oil of A. millefolium subsp. millefolium against the tested fungi. MIC values of the essential oil against the tested fungi ranged from 1.25 μl/ml to 10 μl/ml. The results obtained indicate that essential oil of A. millefolium subsp. millefolium can be used as an alternative to antifungal agents such as amphotericin, ketoconazole, and fluconazole.
DOI: http://dx.doi.org/10.5281/zenodo.1346338
JBródka Publisher
2018-07-03 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/68
European Journal of Biological Research; Vol 8 No 3 (2018): July-September 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/69
2021-02-26T10:51:31Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
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Mycological and enzymatic studies on fresh beef meat sold in Taiz City, Yemen
Abdel-Sater, M. A.
Department of Botany & Microbiology, Faculty of Science, Assiut University, Egypt
Al-Sharjabi, F. A.
Department of Microbiology, Faculty of Applied Science, Taiz University, Yemen
Al-Ashwal, Elham S.
Department of Microbiology, Faculty of Applied Science, Taiz University, Yemen
The mycological analysis of 30 fresh beef meat samples on Czapek’s agar at 7º and 28ºC revealed that, heavily contamination with moulds was observed especially at 28ºC. A total of 234 and 400 colonies ⁄ 450 g meat were collected on both temperatures, respectively. Sixty-seven species belonging to 20 genera were identified. Members of Aspergillus, Mucor, Penicillium and Trichoderma were the most prevalent fungi. At 7°C was highly spoilage by yeasts fungi, while filamentous fungi predominated at 28°C. The ability of the common fungal isolates to produce protease and lipase enzymes revealed that most of them were positive. Among 152 isolates tested, 103 (67.8%) and 96 (63.2%) could respectively produce these enzymes. Because the deteriorative effects of the above fungi, food should be frequently and routinely analyzed. Also, it is essential to store the meat at lower temperature immediately after slaughtering and during transport and storage to reduce or prevent mould growth.
DOI: http://dx.doi.org/10.5281/zenodo.1037238
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/69
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/70
2021-02-26T10:51:30Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
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Immunomodulatory and hematological effects induced by diclofenac, ibuprofen or paracetamol toxicity in Swiss albino mice
Gomaa, Soha
Immunology and Biotechnology Division, Zoology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
Anti-inflammatory drugs (both COX-2 inhibitors and nonselective non-steroidal anti-inflammatory drugs = NSAIDs), paracetamol and opioid agents are associated with potentially different adverse events with varying degrees of efficacy. The present work was conducted to elucidate the haemato-immunological changes in mice when treated with diclofenac (Diclo), ibuprofen (Ibu) and paracetamol (Para). Mice were intraperitoneally administered with Diclo (7.4 mg/kg and 14.8 mg/kg), Ibu (60 mg/kg and 120 mg/kg) or Para (36.7 mg/kg and 73.4 mg/kg) daily for one month against saline-treated mice served as control. Diclo administration (14.8 mg/kg) caused decrease in RBCs count, Hb content and Hct%, depending on dose toxicity, while paracetamol and ibuprofen treatment showed increase in RBCs count, Hb content and Hct%. Additionally, all tested drugs induced activities of IgM and C-reactive protein in serum and caused perturbations in absolute and relative weight of immune related organs. Further, Diclo and Para treatments reduced levels of IgG in dose dependent manner however, Ibu administration enhanced activities of IgG that was reduced with increasing dose of Ibu. And activities of serum complement component C3 was diminished after administration of tested drugs activating alternative complement pathway. The implication of this research is that long use of diclofenac, ibuprofen or paracetamol may cause immunotoxic and hematotoxic effects in mice; and the dose plus the duration of treatment may augment their toxicity probably due to immune modulatory effects. Further studies are needed to assess the relevance between Diclo, Ibu or Para treatment and immunological and hematological perturbations.
DOI: http://dx.doi.org/10.5281/zenodo.1041933
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/70
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/71
2021-02-26T10:51:30Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
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Isozyme variants in two natural populations of Lymnaea luteola
Singh, Arvind Kumar
Department of Zoology, Banaras Hindu University, Varanasi - 221 005, India
Yadav, Naveen
Department of Zoology, Banaras Hindu University, Varanasi - 221 005, India
Singh, Gurvachan
Department of Zoology, Banaras Hindu University, Varanasi - 221 005, India
Lymnaea luteola is a fresh water gastropod snail, inhabiting ponds and lakes of different parts of India. Two populations of L. luteola were collected from fresh water ponds of district Varanasi (Uttar Pradesh) and analysed for their isozyme variants of Xanthine dehydrogenase (Xdh) and Aldehyde oxidase (Ao) enzymes loci. Both enzymes were found to be represented by two distinct loci and each locus of an enzyme showed polymorphic appearance. Based on the electrophoretic variant data, level of heterozygosity was computed for each enzyme locus. Our analysis clearly reveals that L. luteola inhabiting in these two ponds have undergone enough genetic differentiation.
DOI: http://dx.doi.org/10.5281/zenodo.1045133
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/71
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/72
2021-02-26T10:51:29Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
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Virulence genes and antibiotic resistance of Yersinia enterocolitica strains isolated from children
Kot, Barbara
Department of Microbiology, Institute of Biology, Siedlce University of Natural Sciences and Humanities, 12 Bolesława Prusa Str., 08-110 Siedlce, Poland
Piechota, Małgorzata
Department of Microbiology, Institute of Biology, Siedlce University of Natural Sciences and Humanities, 12 Bolesława Prusa Str., 08-110 Siedlce, Poland
Jakubiak, Kinga
Department of Microbiology, Institute of Biology, Siedlce University of Natural Sciences and Humanities, 12 Bolesława Prusa Str., 08-110 Siedlce, Poland
Yersinia enterocolitica is a foodborne pathogen which is primarily responsible for gastrointestinal infections. The presence of the virulence genes in Y. enterocolitica strains isolated from children and antimicrobial resistance was studied in this work. The PCR, biotyping and disc diffusion method were used for analysis of Y. enterocolitica strains. Most of Y. enterocolitica strains belonged to biotype 4 and all carried ail, myfA and ystaA genes. Most of them also had the plasmid yadA gene. These genes were also detected in the strains of biotype 2, while in the two strains of biotype 1A only myfA gene was found. The blaA gene was present in all the strains of biotype 4 and 2, while blaB in the strains of biotype 2 and in some of biotype 4 strains. The presence of β-lactamase genes in Y. enterocolitica was not detected in biotype 1A. All strains were resistant to ampicillin, 76.2% and 47.6% were resistant to ticarcillin and piperacillin, respectively. Two strains (9.5%) were resistant to amoxicillin/ clavulanic acid and aztreonam, three (14.3%) to chloramphenicol, four (19%) to amikacin and trimethoprim/sulfamethoxazole, six (28.6%) to gentamicin. A few strains of Y. enterocolitica were multidrug resistant. The Y. enterocolitica strains isolated from the faeces of children suffering from diarrhea carried virulence genes and some of them were resistant to antibiotics used in extra-intestinal yersiniosis treatment.
DOI: http://dx.doi.org/10.5281/zenodo.1064835
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/72
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/73
2021-02-26T10:51:29Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"171231 2017 eng "
2449-8955
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Ingredients of popular fruit teas in Poland
Adamczak, Artur
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Forycka, Anna
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Karpiński, Tomasz M.
Department of Genetics and Pharmaceutical Microbiology, Poznań University of Medical Sciences, Święcickiego 4, 60-781 Poznań, Poland
Fruit teas are very popular on the market of food products in many countries, due to their attractive taste and aroma as well as pro-health and medicinal properties. They are also characterized by the great wealth and diversity of composition. The purpose of this study was to analyze selected products based on the information contained on their packaging. The research included the most popular fruit teas widely available on the Polish food market, i.e. raspberry, cranberry and rosehip teas, 82 products in total. It was found that plant raw materials appearing in the tea names often constitute a small percentage of their composition, while hibiscus and apple occur very often and in the large quantities. The analysis of the content of the basic ingredient of raspberry and cranberry teas showed that they are characterized by a large diversity of quality. In addition to products with a relatively high amount of raspberry or cranberry (mean: 43.8 and 27.2%, respectively), there were teas with a very low level of these ingredients (mean: 7.5 and 1.6%). Against this background, rosehip tea has stood out positively. In this category of products, Rosa spp. hips, as a widely available plant raw material, most often obtained content above 30-40%.
DOI: http://dx.doi.org/10.5281/zenodo.1175585
JBródka Publisher
2017-12-31 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/73
European Journal of Biological Research; Vol 7 No 4 (2017): October-December 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/78
2021-02-26T10:51:22Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"180910 2018 eng "
2449-8955
dc
Capability of Plant Growth-Promoting Rhizobacteria (PGPR) for producing indole acetic acid (IAA) under extreme conditions
Yousef, Naeima M. H.
Department of Botany & Microbiology, Faculty of Science, Assiut University, 71516 Assiut, Egypt
Plant Growth-Promoting Rhizobacteria (PGPR) inhabiting the area around the plant roots or in plant tissues and stimulate plant growth directly or indirectly. Synthesis of the phytohormone auxin indole-3-acetic acid (IAA) is one of the direct effects of PGPR on plant growth. This study aimed to isolate and screen IAA producing bacteria from soil and study the impacts of the alkalinity and salinity on IAA production and total antioxidant activity of the highly IAA producing strain. From the fifteen isolates tested, six were selected as efficient IAA producer, from which one isolate was highly IAA producer. The highly producing isolate was identified based on molecular characteristics using 16S rRNA. The sequence analysis showed 99% similarity with Bacillus subtilis from GenBank data base. The strain yielded IAA in a wide range of pH (5-9), giving its maximum IAA production at pH 8. High IAA concentration was also observed in the presence of 0.5% and 1% NaCl in comparison with control (with no NaCl). Furthermore, the results indicated that, total antioxidant was increased in acidic (pH 5 and pH 6) and alkaline (pH 8) media, as well as in salinity up to 2%. This study could be stated as the prospective of IAA producing bacterial isolate in the field, as a result, using it as alternative valuable biofertilizer.
DOI: http://dx.doi.org/10.5281/zenodo.1412796
JBródka Publisher
2018-09-10 20:59:15
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/78
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/87
2021-02-26T10:51:20Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181122 2018 eng "
2449-8955
dc
Degradation of surfactant and metal-removal by bacteria from a Nigerian laundry environment
Adekanmbi, Abimbola Olumide
Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
Oyeladun, Wasiu Oyekunle
Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
Olaposi, Adedolapo Victoria
Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
This study aimed at degrading sodium dodecyl sulphate (SDS), a surfactant in the presence of metals using metal-tolerant bacteria from a laundry site. Metal composition of wastewater and sediments from a laundry environment was determined using atomic absorption spectrometry (AAS). Paenibacillus amylolyticus BAL1 (PAB) and Bacillus lentus BAL2 (BLB), earlier reported to tolerate 1000 ppm SDS were screened for metal tolerance. The bacteria were employed in the simultaneous degradation of SDS and metal removal in a batch culture set-up containing SDS and metals for 14 days on a rotary shaker at 250 rpm. Residual SDS and metal concentrations were determined using high performance liquid chromatography (HPLC) and AAS. Copper (Cu), zinc (Zn), and cadmium (Cd) were detected in both laundry wastewater and sediment while chromium (Cr) and nickel (Ni) were only detected in the sediments. The MICs of metals on PAB were: Cu and Zn (500 µg/ml), and Cd (100 µg/ml), while for BLB: Cu (500 µg/ml), Zn (400 µg/ml), and Cd (100 µg/ml). PAB degraded 49.90% of SDS and simultaneously removed 8.3% of Cu, 5.1% of Cd, and 6.6% of Zn, while BLB degraded 54.9% of SDS and simultaneously removed 3.1% of Cu, 39% of Cd, and 3.1% of Zn. A combination of the two bacteria led to 44.3% degradation of SDS, and removal of 11% of Cu, 7.7% of Cd, and 9.8% of Zn. Bacteria from this study possessed both SDS-degradation and metal-removing abilities, and could be useful in the bioremediation of wastewater co-contaminated by surfactants and metals due to their dual tolerance to both compounds.
DOI: http://dx.doi.org/10.5281/zenodo.1494292
JBródka Publisher
2018-09-10 20:59:15
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/87
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/88
2021-02-26T10:51:21Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181003 2018 eng "
2449-8955
dc
Dynamics of oxygen consumption during the formation of the anoxic zone in aquatic environment
Rychert, Krzysztof
Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Arciszewskiego 22b, 76-200 Słupsk, Poland
Mendrzejewska, Beata
Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Arciszewskiego 22b, 76-200 Słupsk, Poland
Kiestrzyń, Anna
Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Arciszewskiego 22b, 76-200 Słupsk, Poland
Anoxic environments and communities of anaerobic organisms are encountered in aquatic environments and biotechnological reactors. Because of their importance, they are continuously studied. In this study, the dynamics of oxygen removal were observed during experiments reproducing the formation of the anoxic zone. Seven experiments were performed in an aquarium (volume: 60 l) with bottom sediments and water collected from different aquatic environments (river, pond, eutrophic lake, sea). To exclude reaeration, the water was isolated from the air by a layer of liquid paraffin. Below the paraffin layer the water was periodically mixed with a stirrer and sampled for oxygen concentration. Initially, a high rate of oxygen consumption was observed. Later, at low oxygen concentrations, the oxygen removal rate switched to a much lower one. Anoxic conditions were observed after 4-20 days of incubation, depending on the experiment. The point at which the microbial community converted from aerobic respiration to anaerobic metabolism was distinct and was observed at an oxygen concentration of 0.26-1.41 mg/l, depending on the experiment. The experiments were accompanied by bacterial counts and analyses of ciliate communities. The study indicates how the disappearance of oxygen during anoxic zone formation should be modeled, and provides data on the oxygen removal rates associated with aerobic and anaerobic communities of microorganisms.
DOI: http://dx.doi.org/10.5281/zenodo.1443571
JBródka Publisher
2018-09-10 20:59:15
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/88
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/91
2021-02-26T10:51:20Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181023 2018 eng "
2449-8955
dc
Anti-oxidant effect of Flemingia stricta Roxb. leaves methanolic extract
Biozid, Md. Shahrear
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Alam, Mohammad Nazmul
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Banglades; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Abeden, Md. Jainul
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh
Chowdhury, Ahmad Ibtehaz
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Faruk, Md.
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Ferdous, Khandoker Usran
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Nitul, Iffat Ara
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Rahman, Md. Masudur
Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh
Aim of the study was to evaluate the possible anti-oxidant activity of Flemingia stricta leaf extract. In antioxidant study, plant crude methanol extract was evaluated for 1,1-diphenyl,2-picrylhydrazyl (DPPH) and reducing power capacity. Moreover, total phenolic and total flavonoid content of plant extracts were determined and expressed in mg of gallic acid equivalent per gram of dry sample (mg GAE/g dry weight). In the DPPH free radical scavenging assay, methanol extract showed concentration dependent inhibition of the free radicals. IC50 of ascorbic acid and F. stricta leaves were 4.25 µg/ml and 320.47 µg/ml respectively. In case of reducing capacity, the methanol extract at concentrations of 25, 50, 100, 200, 400 µg/ml, the absorbances were 0.56, 0.92, 1.41, 1.76, 2.23, respectively. Total phenolic content was estimated by gallic acid and expressed as milligrams of gallic acid equivalent (GAE). The methanol extracts contained a considerable amount of phenolic contents of 482±8.72 of GAE/g of extract and the total flavonoid content of the F. stricta leaf was estimated by using aluminium chloride colorimetric technique and found that the extract contained flavonoid content 340.625±4.50 of GAE/g of extract. These results suggested that the methanol extract of F. stricta Roxb. possess anti-oxidant activity.
DOI: http://dx.doi.org/10.5281/zenodo.1469767
JBródka Publisher
2018-09-10 20:59:15
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/91
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/93
2021-02-26T10:51:20Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181106 2018 eng "
2449-8955
dc
Biochemical composition and antioxidant properties of some seaweeds from Red Sea coast, Egypt
El-Shazoly, Rasha M.
Botany and Microbiology Department, Faculty of Science, New Valley University, 72511, Al-Kharja, New Valley, Egypt
Fawzy, Mustafa A.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt
The current study investigated the biochemical composition and antioxidant properties of four seaweeds: Laurencia sp. (Rhodophyta), Cystoseira myrica, Hydroclathrus clathratus and Padina pavonica (Ochrophyta). The highest amount of carbohydrates was (215.78 mg/g dry wt.) in Laurencia sp. and proteins content was maximum (50 mg/g dry wt.) in Laurencia sp. and Cystoseira myrica. The highest values of free amino acid content were recorded in the brown seaweed species Cystoseira myrica (4.01 mg/g dry wt.). The pressurized hot water extract of Cystoseira myrica has the highest total phenolic content (1.61 mg GAE/g dry wt.). Cystoseira myrica contained the highest amounts of flavonoids (3.35 mg/g dry wt.), ascorbic acid (9.07 mg/g dry wt.) and α-tocopherol (27.25±0.00 abs. at 520 nm/g dry wt.). Furthermore, the ethyl alcohol extract of Cystoseira myrica showed high antioxidant capacities (541.6 mg/g dry wt.) and achieved the most powerful reducing ability among all of the different extracts of algal species. Statistical evaluation by Spearman correlation between the TAC assay and the total phenolic contents was found to be significant, but the correlation was nonsignificant between FRAP assay and the total phenolic contents. The composition of elements of the studied seaweed species was also analyzed. The most significant macro-elements present in the studied seaweeds were K, Na and Ca, representing that the seaweeds are good sources of these elements. Since, these seaweeds are widespread in the Egyptian waters, their biochemical composition and antioxidant capacities made them promising candidates for industrial, nutritional and pharmaceutical applications.
DOI: http://dx.doi.org/10.5281/zenodo.1478863
JBródka Publisher
2018-09-10 20:59:15
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/93
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/95
2021-02-26T10:51:21Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181012 2018 eng "
2449-8955
dc
Heavy metal composition in the Plantago major L. from center of the Murmansk City, Kola Peninsula, Russia
Huber, Miłosz Andrzej
Department of Geology and Lithosphere Protection, Faculty of Earth Science and Spatial Management, Maria Curie-Skłodowska University, 2d Kraśnicka St., Lublin, Poland
Menshakova, M. Y.
Department of Natural Sciences, Murmansk Arctic State University, 6 Kommuny St., Murmansk, Russia
Chmiel, S.
Department of Hydrography, Faculty of Earth Science and Spatial Management, Maria Curie-Skłodowska University, 2d Kraśnicka St., Lublin, Poland
Zhigunova, G. V.
Department of Philosophy and Social Sciences, Murmansk Arctic State University, 15 Kapten Egorov St. Murmansk, Russia
Dębicki, R.
Department of Soil Science and Soil Protection, Faculty of Earth Science and Spatial Management, Maria Curie-Skłodowska University, 2d Kraśnicka St., Lublin, Poland
Iakovleva, О. А.
Department of Russian Language, Slavian Philology Institute, Humanist Faculty, Maria Curie-Skłodowska University, Maria Curie Skłodowska Sq., Lublin, Poland
Plantago major is an indicator of environmental pollution in the city. The plant grows along the traversed paths, close to the sidewalks. Contaminating substances accumulate on the leaves of the plantain. In the summer of 2016, samples of plants were collected in the central Murmansk region for analysis using a scanning electron microscope to identify dust particles on their surface, and to study leaves using the ICP-MS method to determination of heavy metals content. A relatively serious concentration of lead, zinc, copper, nickel as well as high arsenic and chromium content has been demonstrated in the city center, along with ties with human activities (vehicular traffic). High iron content is associated with peat soils used in the city for fertilization. The remaining metal content is relatively low.
DOI: http://dx.doi.org/10.5281/zenodo.1461064
JBródka Publisher
2018-09-10 20:59:15
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/95
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/102
2021-02-26T10:51:19Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190121 2019 eng "
2449-8955
dc
Phenolics content, antiproliferative and antioxidant activities of Algerian Malva sylvestris
Boutennoun, Hanane Messaoud
Molecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, PB 98, Ouled Aissa, 1800, Jijel, Algeria; Biomathematics, Biophysics, Biochemistry and Scientometry Laboratory, Life and Nature Sciences Faculty, University of Bejaia, 06000 Bejaia, Algeria
Boussoufe, Lilia
Molecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, PB 98, Ouled Aissa, 1800, Jijel, Algeria; Biomathematics, Biophysics, Biochemistry and Scientometry Laboratory, Life and Nature Sciences Faculty, University of Bejaia, 06000 Bejaia, Algeria
Kebieche, Mohamed
Microbiology and Biochemistry Department, Faculty of Nature and Life Sciences, University of Batna 2, Algeria
Al-Qaoud, Khaled
Molecular Immunoparasitology Laboratory, Department of Biological Sciences, Faculty of Science, University of Yarmouk, Irbid, Jordan
Madani, Khodir
Biomathematics, Biophysics, Biochemistry and Scientometry Laboratory, Life and Nature Sciences Faculty, University of Bejaia, 06000 Bejaia, Algeria
Due to its expected low toxicity to human use, more attention is given worldwide to antioxidants of natural sources. Therefore, the extraction of the total phenolic compounds contained in the leaves of Malva sylvestris and the analysis of the polyphenols, flavonoids and tannins contents were carried out. The antioxidant activity of the hydro-methanolic extract of Malva sylvestris was investigated employing various established in vitro systems including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, the reduction of hydrogen peroxide and the ferric reducing power assay. The antiproliferative activity of plant extract was tested against three tumor cell lines: MCF-7, Hep2 and WEHI using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay. Preliminary screening indicated the presence of substances with large therapeutic values: an important content of polyphenols, flavonoids and tannins was detected in the tested extract. Our data showed that the extract exhibited high antioxidant properties, which were demonstrated by its ability to scavenge 76.11% of DPPH free radicals, and the elimination of 69.97% of hydrogen peroxide at a concentration of 125 µg/ml. In addition, the plant extract showed strong ferric reducing power which was a function of the sample concentration. For the antiproliferative activity, the results demonstrated that the plant extract significantly inhibited tumor cell growth and colony formation in a concentration-dependent manner. The toxicity percentage of extract at 125 µg/ml on MCF-7, Hep2 and WEHI was found in the order of 45.20%, 62.62% and 82.04%, respectively.
DOI: http://dx.doi.org/10.5281/zenodo.2545914
JBródka Publisher
2019-01-15 20:58:01
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/102
European Journal of Biological Research; Vol 9 No 1 (2019): January-March 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/103
2021-02-26T10:51:19Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"181213 2018 eng "
2449-8955
dc
Community structure of mesofauna in the light of qualitative and quantitative research on soil mites
Błoszyk, Jerzy
Department of General Zoology, Faculty of Biology AMU in Poznań, ul. Umultowska 89, 61-614 Poznań, Poland
Napierała, Agnieszka
The Natural History Collections, Faculty of Biology AMU in Poznań, ul. Umultowska 89, 61-614 Poznań, Poland
Research into structure and abundance of soil fauna communities should be based on material consisting of both qualitative and quantitative samples to provide reliable results. However, in practice it turns out that sometimes it is simply impossible to have both qualitative and quantitative samples. The study presents a comparative analysis of results obtained with qualitative and quantitative methods used in research into soil mites from the suborder Uropodina (Acari: Mesostigmata). The research was carried out in different regions of Poland. Both qualitative (sieving of soil and litter) and quantitative samples were collected in each of the examined ground plots. The results presented in the study show that zoocenological analysis based on both qualitative and quantitative samples gives similar results in the case of common and abundant species, and collecting 2 or 3 sievings in a given ground plot can be equivalent to large series of quantitative samples in faunistic research and monitoring of the environment. This stems from the fact that sieving of litter allow to obtain far more dense material than from quantitative samples. Due to the high density of sieving they contain more species and specimens, including specimens at different developmental stages found in the examined area. This type of sampling can be more efficient when the researcher needs a simple and fast method of collecting material for analysis, especially in the case of extensive research conducted in large areas, monitoring of changes in soil, as well as in taxonomic, biometric, biogeographical, and molecular research.
DOI: http://dx.doi.org/10.5281/zenodo.2248744
JBródka Publisher
2018-09-10 20:59:15
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/103
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/108
2021-02-26T10:51:19Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190115 2019 eng "
2449-8955
dc
Therapeutic effect of fractionated by ultrafiltration red beetroot (Beta vulgaris L.) juice in rats with food-induced fatty liver
Babarykin, Dmitry
Institute of Innovative Biomedical Technology Ltd., Riga, Latvia
Smirnova, Galina
Institute of Innovative Biomedical Technology Ltd., Riga, Latvia; Institute of Biology of the University of Latvia, Salaspils, Latvia
Markovs, Jurijs
Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga
Vasiljeva, Svetlana
Institute of Biology of the University of Latvia, Salaspils, Latvia
Basova, Natalija
Institute of Biology of the University of Latvia, Salaspils, Latvia
Simanis, Raimonds
Riga Stradins University, Riga, Latvia
Viksna, Ludmila
Riga Stradins University, Riga, Latvia
The prevalence of non-alcoholic fatty liver disease (NAFLD), being a component of metabolic syndrome, has increased (15-27%) in the industrialized world. The deep mechanism of this pathology is not clear, but it is multifactorial. There is a huge amount of food supplements and medicines with hepatoprotective effect on the market, but the NAFLD problem is far from being resolved. Hepatoprotective products have to provide wide spectra of biological effects, including antioxidant, hypolipidemic, anti-inflammatory action. It is peculiar to natural compounds, including red beetroot juice, which is well known to most of the population. This is important in view of the high prevalence of NAFLD. The aim of this study is to evaluate the curative effect of fractionated by ultrafiltration red beetroot juice in rats with food-induced liver steatosis.
DOI: http://dx.doi.org/10.5281/zenodo.2541075
JBródka Publisher
2019-01-15 20:58:01
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/108
European Journal of Biological Research; Vol 9 No 1 (2019): January-March 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/111
2021-02-26T10:51:18Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190306 2019 eng "
2449-8955
dc
Production and purification of extreme xylanase from Aspergillus flavus AUMC 10331 in sub-merged fermentation
Moubasher, AH
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71511, Egypt; Assiut University Mycological Centre (AUMC), Assiut University, Assiut 71511, Egypt
Ismail, Mady
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71511, Egypt; Assiut University Mycological Centre (AUMC), Assiut University, Assiut 71511, Egypt
Mohamed, Ramadan
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71511, Egypt
Al-Bedak, Osama
Assiut University Mycological Centre (AUMC), Assiut University, Assiut 71511, Egypt
Xylan, extracted from oat spelts in a previous work, was assayed by HPLC and used as carbon source for the production of xylanase from Aspergillus flavus AUMC 10331. The produced xylanase was purified using ion exchange resin (IR-120 EP) and gel filtration column of Sephadex G-75 and Sephadex G-100. The purified xylanase showed total activity of 5.5 IU/ml and specific activity of 687.5 IU/mg, and the enzyme purified 156.75 fold with 4.43 % yield. The highest activity at pH 7.0 and 10.5 indicating two xylanases with the most interesting one with a maximum activity at pH 10.5 and 65 °C. The enzyme activity was greatly stimulated by 5 mM of FeSO4 and CuSO4, while slightly inhibited by other metal ions. Km and Vmax were determined as 8.36 mg/ml and 172.4 IU/min respectively.
DOI: http://dx.doi.org/10.5281/zenodo.2586103
JBródka Publisher
2019-01-15 20:58:01
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/111
European Journal of Biological Research; Vol 9 No 1 (2019): January-March 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/122
2021-02-26T10:51:17Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190411 2019 eng "
2449-8955
dc
Screening for antifungal activity of garlic (Allium sativum) powder against mycelia growth of three post-harvest pathogens
Oladele, Oluwole Olakunle
Department of Biology, Federal Universsity of Technology, Akure, Nigeria
Screening for antifungal activity of garlic powder against mycelia growth of three post-harvest pathogens (Aspergillus, Rhizopus and Mucor species) was investigated in this study. Five grams of malt extract agar (MEA) were poured into a conical flask, 100 ml of water and different weight of garlic powder (1, 3, 5 and 7 g) were separately added, stirred and later sterilized while MEA medium with no garlic added (0 g) served as control. The mycelia of each post-harvest pathogen was cut with 6mm cork borer and placed on the solidified medium in the Petri dish and incubated at 28±2oC for 72 hours. Phytochemical screening of the garlic powder was also investigated. Results from this study showed that the different weights of the garlic powder apart from the control (0 g garlic) significantly inhibited the mycelia growth of the three post-harvest pathogens tested in the study and the order of antifungal activity of the garlic powder against mycelia growth of Aspergillus, Rhizopus and Mucor species was 7 g > 5 g > 3 g > 1 g > 0 g, 5 g > 7 g >1 g > 3 g > 0 g and 7 g > 5 g > 3 g > 1 g > 0 g respectively. The antifungal activity of the garlic powder may be related to the presence of active antimicrobial agents including alkaloids, saponins, tannins, flavonoids and cardiac glycosides that were detected in the powder.
DOI: http://dx.doi.org/10.5281/zenodo.2635824
JBródka Publisher
2019-04-11 00:25:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/122
European Journal of Biological Research; Vol 9 No 2 (2019): April-June 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/123
2021-02-26T10:51:17Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190323 2019 eng "
2449-8955
dc
Removal of hydrocarbons from crude oil contaminated agricultural soil by phytoremediation using Mariscus alternifolius and Fimbristylis ferruginea
Chukwuma, Chukwuemeka C.
Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B. 5239, Choba, Rivers State, Nigeria
Ikewuchi, Jude Chigozie
Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B. 5239, Choba, Rivers State, Nigeria
Monanu, M. O.
Department of Biochemistry, Faculty of Science, University of Port Harcourt, P.M.B. 5239, Choba, Rivers State, Nigeria
Crude oil extraction is one major route through which hydrocarbons are released into the environment and hydrocarbon contamination is highly hazardous to the ecosystem. This study investigated the removal of hydrocarbons from crude oil contaminated agricultural soils using Mariscus alternifolius Vahl. and Fimbristylis ferruginea plant species. Before planting, the polluted soil (negative control) had a total petroleum hydrocarbon concentration of 17962.11±1000.00 mg/kg and polycyclic aromatic hydrocarbon concentration of 440.97±1.00 mg/kg. Likewise, the soil oil and organic carbon contents were 3.25±0.10 ppm and 3.06±0.02% respectively. Results, 90 days after planting, indicated a significant decrease in the total petroleum hydrocarbon concentrations of M. alternifolius (100.82±46.31 mg/kg) and F. ferruginea (110.41±39.68 mg/kg) treated soils. Likewise, there was a significant decrease in the polycyclic aromatic hydrocarbon concentration of M. alternifolius treated soil (95.69±65.44 mg/kg). The oil content of the treated soils significantly decreased to 1.03±0.28 ppm and 0.84±0.33 ppm in M. alternifolius and F. ferruginea treated soils respectively, while the organic content of the treated soils significantly decreased to 2.16±0.09% and 2.20±0.20% in M. alternifolius and F. ferruginea treated soils respectively. Phytoremediation using M. alternifolius and F. ferruginea has proven to be potent in the remediation of hydrocarbon contaminated soil through enhancement and recovery of the polluted soil. These plant species which improved the cultivation and germination competence of the treated soils thus making the soil probable for agricultural and other related purposes are therefore recommended for used in the phytoremediation of crude oil contaminated soils.
DOI: http://dx.doi.org/10.5281/zenodo.2603912
JBródka Publisher
2019-01-15 20:58:01
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/123
European Journal of Biological Research; Vol 9 No 1 (2019): January-March 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/126
2021-02-26T10:51:18Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190131 2019 eng "
2449-8955
dc
Contribution to the bryoflora of the Chochołowska Valley in the Polish Tatra Mountains
Karpiński, Tomasz M.
Department of Medical Microbiology, Poznań University of Medical Sciences, Wieniawskiego 3, 61-712 Poznań, Poland
Adamczak, Artur
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland
Rusińska, Anna
Natural History Collections, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
The paper presents a list of 64 moss species recorded in the Chochołowska Valley (including Wyżnia Chochołowska Valley, Jarząbcza Valley and the surrounding peaks). Detected taxa belong to 25 families. Among them, the most commonly represented are Polytrichaceae (9 species), Dicranaceae (6), Pottiaceae (6), Hylocomiaceae (5), Hypnaceae (5), Grimmiaceae (4), and Bryaceae (4). Mosses were collected from different substrates, but they usually grew on humus (37 taxa), which sometimes covered with a thin layer of granite or limestone gravel. 15 species were found on epilithic habitats, especially on limestone rocks (10), while 8 species occurred on wood or tree trunks. Some mosses occupied synanthropic habitats (6 species). For example, apophytes widely distributed in the lowlands: Tortula muralis, Dryptodon pulvinatus, and Schistidium crassipilum were recorded on the wall near the PTTK shelter on the Chochołowska Glade, whereas an oreoapophyte – Pogonatum urnigerum grew on the path in Wyżnia Chochołowska Valley.
DOI: http://dx.doi.org/10.5281/zenodo.2546974
JBródka Publisher
2018-09-10 20:59:15
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/126
European Journal of Biological Research; Vol 8 No 4 (2018): October-December 2018
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/127
2021-02-26T10:51:36Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170930 2017 eng "
2449-8955
dc
Enhancement of alpha amylase production by Aspergillus flavus AUMC 11685 on mandarin (Citrus reticulata) peel using submerged fermentation
Ali, Esam H.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt
El-Nagdy, Mohamed A.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt
Al-Garni, Saleh M.
Microbiology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Ahmed, Mohamed S.
Microbiology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
Rawaa, Ahmed M.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt
Mandarin peel as submerged fermentation (SmF) source was tested for the production of alpha amylase enzyme by strain of Aspergillus flavus AUMC 11685. Incubation period, concentration of substrate, temperature, pH and size of inoculum were optimized to achieve the maximum production of alpha amylase enzyme by Aspergillus flavus using mandarin peel. The maximum production of alpha amylase enzyme by Aspergillus flavus was recorded at 4-5 days of incubation, 3% substrate concentration, inoculum concentration 10%, temperature 28-40°C and pH 4-5.5.
DOI: http://dx.doi.org/10.5281/zenodo.818271
JBródka Publisher
2017-09-30 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/127
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/130
2021-02-26T10:51:36Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170930 2017 eng "
2449-8955
dc
Influence of extracellular matrix on the proliferation and adhesion properties of stem cells derived from different sources
Bajek, Anna
Department of Tissue Engineering, Nicolaus Copernicus University, Collegium Medicum Bydgoszcz, Poland
Porowińska, Dorota
Department of Biochemistry, Nicolaus Copernicus University, Toruń, Poland
Roszkowski, Krzysztof
Department of Oncology, Radiotherapy and Oncological Ginecology, Nicolaus Copernicus University, Romanowskiej 2, 85-796 Bydgoszcz, Poland
One of the most important issues in regenerative medicine is the development of culture conditions mimicking the natural ones, which allows obtaining a large number of cells and their long-term maintenance in undifferentiated state. In vivo, cells are surrounded by a specific microenvironment called extracellular matrix (ECM), which plays an important role in the regulation of processes such as proliferation, migration, differentiation or apoptosis. In this study we assessed the influence of different extracellular matrix components (fibronectin, laminin, collagen IV, poly-D-lysine) on the in vitro adhesion and proliferation of stem cells isolated from bone marrow, adipose tissue and hair follicles. Our results showed that stem cells derived from different sources present various responses to ECM components. None of the tested extracellular proteins reduced the proliferation of bone marrow as well as adipose-derived mesenchymal stem cells, with the exception of laminin. This demonstrates the biocompatibility of such modified surfaces and possibility of using them for culturing these types of stem cells. Different results were obtained for hair follicle stem cells. The presented results indicate that ECM is an important component of the cellular niche in the tissue. It is also possible that ECM is required for the reconstitution of the niche of stem cells in vitro.
DOI: http://dx.doi.org/10.5281/zenodo.820876
JBródka Publisher
2017-09-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/130
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/131
2021-02-26T10:51:35Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170930 2017 eng "
2449-8955
dc
Functional assessments and histopathology of hepatorenal tissues of rats treated with raw and processed herbs
Ojiako, Okey A.
Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
Chikezie, Paul C.
Department of Biochemistry, Imo State University, Owerri, Nigeria
Ukairo, Doris I.
Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
Ibegbulem, Chiedozie O.
Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
Nwaoguikpe, Reginald N.
Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
The present study ascertained the functional integrity of hepatic and renal tissues, concurrently with blood lipid patterns, of Wistar rats infused with CCl4 and treated with raw and hydrothermal processed herbs, namely, Monodora myristica, Chromolaena odorata, Buccholzia coriacea and Sphenostylis stenocarpa. Measurement of phytochemical contents of the herbs was according to standard methods. The rats were randomly designated on the bases of diets and treatments received for 28 consecutive days. Fibrosis was induced in the Wistar rats by single dose intra-peritoneal injection of CCl4 for 2 consecutive days. Liver and kidney function tests and serum lipid profile were measured using spectrophotometric methods. Renal and hepatic tissues were subjected to histopathological examinations. The concentrations of alkaloids in the four herbal extracts were within the range of 4.83±0.03 - 31.33±0.29 mg/100 g sample, whereas the concentrations of saponins varied within a relatively narrow range: 0.33±0.09 - 4.33±0.02 mg/100 g dry sample; p > 0.05. The activity ratios of AST to ALT of the rat groups were generally less than 1.0 unit. Atherogenic indices of fibrotic rats were within the following ranges: TAG/HDL-C ratio (3.59±0.03 - 6.76±0.06), TC/HDL-C ratio (3.72±0.02 - 6.94±0.05) and LDL-C/HDL-C ratio (2.00±0.01 - 4.59±0.02). Losses in phytochemical contents following hydrothermal processing of the herbs did not substantially affect their overall therapeutic scores against morpho-logical and functional impairments of hepatic and renal tissues following CCl4 intoxication of the rats.
DOI: http://dx.doi.org/10.5281/zenodo.823220
JBródka Publisher
2017-09-30 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/131
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/134
2021-02-26T10:51:35Z
ejbr:RESEARCH
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"170930 2017 eng "
2449-8955
dc
In vitro regeneration of plantlets from nodal explants of Aristolochia saccata and Aristolochia cathcartii
Sarma, Bhaskar
Plant Tissue Culture Laboratory, Department of Botany, Gauhati University, Guwahati 781014, Assam, India
Tanti, Bhaben
Plant Tissue Culture Laboratory, Department of Botany, Gauhati University, Guwahati 781014, Assam, India
In vitro propagation of Aristolochia saccata and A. cathcartii were carried out using nodal explant. In both the plants, nodal explants showed direct somatic embryogenesis when cultured on MS medium using various concentrations of BAP (1.0-4.0) and 2iP (1.0-4.0) separately or in combination with low concentration (0.5 and 1.0 mg l-1) of auxin (NAA). It was observed that BAP in combination with NAA was more effective for shoot induction than the hormones used separately. Among different combinations of NAA and BAP, 3.0 mg/l BAP + 1.0 mg/l NAA showed better response in case of A. saccata of about 96%, whereas, in A. cathcartii, the best response was achieved in 4.0 mg/l BAP + 0.5 mg/l NAA after 28 day of culture and 88.3% explants showed proliferation in this combination. The auxins NAA and IBA were used singly to induce rooting from in vitro raised shoot lets. A range of concentration was tested (0.1, 0.5, 0.8 and 1.0 mg/l) for rooting. In the present study 1/2 strength MS basal medium and the two different auxins (NAA and IBA) were tried, the maximum results on rooting were obtained on half strength with IBA (0.5 mg/l) then NAA.
DOI: http://dx.doi.org/10.5281/zenodo.825746
JBródka Publisher
2017-09-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/134
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/136
2021-02-26T10:51:35Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170930 2017 eng "
2449-8955
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Proposal for screening of kidney disease in a random population based on World Kidney Day campaign
Bacci, Marcelo Rodrigues
Department of General Practice, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Jordão, Victor do Couto Rosa
Department of General Practice, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Vasconcelos, Livia Yadoya
Department of General Practice, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Castanheira, Thiago Cavenaghi
Department of General Practice, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Bergamo, Ronaldo Roberto
Department of Nephrology, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
dos Santos, Daniel Rinaldi
Department of Nephrology, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Mottecy, Ana Carolina Capuano
Department of General Practice, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Azzalis, Ligia Ajaime
Department of Pharmaceutical Scienses of Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil
Pereira, Edimar Cristiano
Department of Pharmaceutical Scienses of Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil
Alves, Beatriz da Costa Aguiar
Clinical Analysis Laboratory, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Fonseca, Fernando Luiz Affonso
Department of Pharmaceutical Scienses of Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil; Clinical Analysis Laboratory, Faculdade de Medicina do ABC, Santo André, Sao Paulo, Brazil
Despite the advances on early screening techniques, getting to know the chronic kidney disease (CKD) prevalence in Brazil and worldwide remains a challenge for researchers. Aging, diabetes and hypertension are the main CKD causes in Brazil. The aim of the study was to evaluate the presence of urinary dipstick abnormalities in World Kidney Day campaign. This is a cross-sectional studyconducted at FMABC. This study was based on the answers to a kidney disease questionnaire and urinary dipstick test. A total of 205 patients were randomly invited to collect urine samples on World Kidney Day 2013. Among the 205 studied patients, 66.34% were women with mean age of 46.32 years. Around 34.14% of the patients were hypertensive and 9.75% diabetic. Urinalysis alterations were observed in 28.29% of patients. The group with urine alterations had older individuals (51.36 years) andmore diabetes (18.96%) with higher levels of glucose (143.2 mg/dl). Brazilian population is getting older and diabetic which represent risk factors for the onset of CKD. The necessity of an early detection by means of specific campaigns is thus of great importance. The use of dipstick test for screening is an important tool for kidney disease diagnosis.
DOI: http://dx.doi.org/10.5281/zenodo.826928
JBródka Publisher
2017-09-30 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/136
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/152
2021-02-26T10:51:16Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190525 2019 eng "
2449-8955
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Antifungal activity of the rhizome extracts of Pulsatilla vulgaris against Candida glabrata
Łaska, Grażyna
Department of Agri-Food Engineering and Environmental Management, Białystok University of Technology, Wiejska 45A, 15-351 Białystok, Poland
Sienkiewicz, Aneta
Department of Agri-Food Engineering and Environmental Management, Białystok University of Technology, Wiejska 45A, 15-351 Białystok, Poland
Pulsatilla vulgaris Mill. (“Pasque flower”, Ranunculaceae) is rare and a threatened plant species in Europe. It produces biologically active secondary metabolites. P. vulgaris is also known herbal drug used for centuries in traditional Chinese and Korean medicine. The rhizomes of P. vulgaris have been traditionally used for treatment of headaches, neuralgia, insomnia, hyperactivity, bacterial skin infections, septicemia, cough and bronchitis. In the present study, the extracts of leaves and rhizomes of P. vulgaris were evaluated for their antifungal, antimicrobial, antimalarial and cytotoxic activities. The results showed the antifungal activity of crude extracts of the rhizome of P. vulgaris against the yeast Candida glabrata with an IC50 of 11 µg/ml. These results indicate that the selected medicinal plant could be further investigated for identifying compounds that may be responsible for the observed activity and that may represent new leads in fungal drug discovery.
DOI: http://dx.doi.org/10.5281/zenodo.3228890
JBródka Publisher
2019-04-11 00:25:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/152
European Journal of Biological Research; Vol 9 No 2 (2019): April-June 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/162
2021-02-26T10:51:15Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190612 2019 eng "
2449-8955
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Immunological exploration based studies on Strychnos nux-vomica regarding antigen specific immune response
Gupta, Amit Joginder
Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
In an effort to determine its cytotoxicity as well as antigen specific immune activity of aqueous leaves extract of Strychnos nux-vomica using hepatitis B vaccine containing surface antigen (HBsAg; 20 µg/ml) pertaining to antibody production and scrutinize its proliferative response along with cytokines in lysed human whole blood. For these studies, phytochemical (qualitative) analysis was determined and evaluates the presence of secondary metabolites through high performance thin layer chromatography (HPTLC) and bio-inorganic fingerprinting. In addition, indirect Elisa was performed using HBsAg as coating antigen using variable doses (1-30 mg/ml) of Strychnos nux-vomica. In continuation of these immunological studies, antigen specific immune response along with cytotoxicity was determined through MTT assay in infected human whole blood using HBsAg (20 µg/ml, 50 µl). The results showed that Strychnos nux-vomica showed qualitatively as well as quantitatively determined the presence of secondary metabolites along with bio-inorganic compounds. In addition, Strychnos nux-vomica showed enhancement in anti-HBsAg IgG titre as compared to standard and control but there is sudden decline in proliferation with HBsAg and also showed decline in cytokines (IL-2 and IL-12) level at higher doses as compared to control. Our data suggest that Strychnos nux-vomica may help to raise antibodies against HBsAg but sudden decline in HBsAg proliferative response along with cytokines (IL-2 and IL-12) in infected lysed human whole blood and also showed some cytotoxic effect at higher doses. In other words, Strychnos nux-vomica could be a potent immune enhancer of B cells and inhibitor of T cells against HBsAg.
DOI: http://dx.doi.org/10.5281/zenodo.3244227
JBródka Publisher
2019-04-11 00:25:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/162
European Journal of Biological Research; Vol 9 No 2 (2019): April-June 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/168
2021-02-26T10:51:16Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190515 2019 eng "
2449-8955
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Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum
Othman, Nasim A. R. M.
Biology Department, Faculty of Science, Taiz University, Taiz, Yemen
Here was investigated the incorporation of copper in MS medium on growth, and metabolic activities of Solanum nigrum callus. Copper up to 75 µM increased the growth, and thereafter a decline was observed. No considerable alteration in MDA, H2O2, bound phenolics, flavonoids, ascorbate, and copper content was observed with the existence of 25 µM copper, then levels of these parameters were raised with rising copper concentrations. Similarly, 25 µM copper didn't induce a considerable change in lipoxygenase, superoxide dismutase, catalase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase activities, however, high levels stimulated these enzymes. Copper at 25 µM didn’t considerably reduce amino acids and soluble proteins, whereas higher concentrations reduced these parameters. Copper treatments reduced the soluble carbohydrates accumulation; only 75 µM enhanced this accumulation. Copper at 25 µM significantly increased the potassium accumulation, whereas higher concentrations reduced this accumulation. From these results, it might be contemplated the optimum effect concerning copper.
DOI: http://dx.doi.org/10.5281/zenodo.2848646
JBródka Publisher
2019-04-11 00:25:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/168
European Journal of Biological Research; Vol 9 No 2 (2019): April-June 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/170
2021-02-26T10:51:34Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170930 2017 eng "
2449-8955
dc
In vitro assessment of antimicrobial and anti-inflammatory potential of endophytic fungal metabolites extracts
Moharram, A. M.
Department of Botany & Microbiology, Faculty of Science, Assiut University, Assiut, Egypt
Zohri, A. A.
Department of Botany & Microbiology, Faculty of Science, Assiut University, Assiut, Egypt
Omar, H. M.
Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt
Abd El-Ghani, O. A.
Department of Botany & Microbiology, Faculty of Science, Assiut University, Assiut, Egypt
Endophytes are endosymbiotic microorganisms that act as reservoir of novel bioactive secondary metabolites with antimicrobial, cytotoxic and anti-cancer activities. In the present study, the extracts of 26 different endophytic fungal strains were screened for their antimicrobial and anti-inflammatory activities. The results showed a wide variety of antimicrobial activities against 12 target microorganisms including three Gram (+) bacteria, three Gram (-) bacteria, 3 yeasts, 2 dermatophytic fungi and one keratinophilic fungus. Four fungal extracts (Aspergillus versicolor, A. awamori, A. niger and Penicillium funiculosum) displayed a broader antibacterial spectrum and inhibited the growth of all Gram (+) and Gram (-) bacterial species. The extracts of 8 endophytic fungi inhibited the growth of the two tested dermatophytic strains (Trichophyton mentagrophytes and T. rubrum). Only eight fungal extracts have an inhibition activity against the keratinophilic fungal strain (Chrysosporium tropicum). The anti-inflammatory assay showed that the extracts of Emericella nidulans, Pleospora tarda and Penicillium funiculosum had good activities in inhibition of protein denaturation reached to 83%, 82.5% and 81.4%, respectively. Also, Emericella nidulans and Pleo-spora tarda recorded the maximum inhibition effect on bovine serum albumin denaturation reached to 95% and 90.7%, respectively. On the other side, Emericella nidulans showed the maximum inhibition activity (69.5%) out of all tested endophytic strains against humun red blood cells membrane stabilization assay. In conclusion some secondary metabolites of endophytic fungi have a promising potential as antimicrobial and anti-inflammatory compounds.
DOI: http://dx.doi.org/10.5281/zenodo.839696
JBródka Publisher
2017-09-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/170
European Journal of Biological Research; Vol 7 No 3 (2017): July-September 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/174
2021-02-26T10:51:38Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170630 2017 eng "
2449-8955
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Efficiency of consortium for in-situ bioremediation and CO2 evolution method of refines petroleum oil in microcosms study
Dutta, Shreyasri
Department of Microbiology, Gurukul Kangri University (KGC), Haridwar, Uttrakhand-249404, India
Singh, Padma
Department of Microbiology, Gurukul Kangri University (KGC), Haridwar, Uttrakhand-249404, India
An in-situ bioremediation study was conducted in a laboratory by using mixed microbial consortium. An indigenous microbial consortium was developed by assemble of two Pseudomonas spp. and two Aspergillus spp. which were isolated from various oil contaminated sites of India. The laboratory feasibility study was conducted in a 225 m2 block. Six treatment options: Oil alone, Oil+Best remediater, Oil+Bacterial consortium, Oil+Fungal consortium, Oil+Mixed microbial consortium, Oil+Indigenous microflora. Out of five treatments, the mixed microbial consortium (Block 5) degraded 55.12% refine petroleum oil compare to degradation of bacterial (Block 3) and Fungal consortium (Block 4) (i.e, degradation rate were 19.88% and 18.07% correspondingly) after the end of treatment (60 days). Bioremediation ability of this consortium was confirmed by CO2 evolution method. The result showed that 136.36 % CO2 release after 12 days incubation. 16r DNA sequencing showed that two bacterial species were Pseudomonas aeruginosa and morph taxonomical examination of fungus were Aspergillus terrus (At) and Aspergillus flavus (Af).The ability of degradation of synthetic mixture of refine petroleum oils makes the consortium potentially useful for bioremediation and microbial enhanced oil recovery.
DOI: http://dx.doi.org/10.5281/zenodo.501069
JBródka Publisher
2017-06-30 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/174
European Journal of Biological Research; Vol 7 No 2 (2017): April-June 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/176
2021-02-26T10:51:38Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170630 2017 eng "
2449-8955
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Evaluation of antiplasmodial effects of the ethanolic leaf extract of Salacia lehmbachii on Plasmodium berghei infected mice
Essien, A. D.
Department of Pharmacology, College of Medical Sciences, University of Calabar, Nigeria
Essiet, G. A.
Department of Pharmacology, College of Medical Sciences, University of Calabar, Nigeria
Akuodor, G. C.
Department of Pharmacology and Therapeutics, Faculty of Medicine, Ebonyi State University, Abakaliki, Nigeria
Nwobodo, N. N.
Department of Pharmacology and Therapeutics, Faculty of Medicine, Ebonyi State University, Abakaliki, Nigeria
Akpan, J. L.
Department of Pharmacology and Therapeutics, Faculty of Medicine, Ebonyi State University, Abakaliki, Nigeria
Utsalo, S. J.
Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, University of Calabar, Nigeria
Salacia lehmbachii leaves are used in Nigerian traditional medicine for the treatment of malaria and other diseases. The ethanolic extract was tested for its activities against suppressive, prophylactic and established infections in Plasmodium berghei infected albino mice at dose levels of 100, 200 and 400 mg/kg; while chloroquine (10 mg/kg) was used as positive control. The extract exhibited significant dose-related antiplasmodial activities on parasites with the used-dose levels, showing significant mean survival time. The results, therefore, co-relate with claims by traditional users for the treatment of malaria and other feverish conditions; and could serve as source of potential new antimalarial agents.
DOI: http://dx.doi.org/10.5281/zenodo.556102
JBródka Publisher
2017-06-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/176
European Journal of Biological Research; Vol 7 No 2 (2017): April-June 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/177
2021-02-26T10:51:37Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170630 2017 eng "
2449-8955
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Alteration in biochemical indices following administration of seafood (Thais coronata) extract
Archibong, A. N.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Akwari, A. A.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Ofem, O. E.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Bassey, I. O.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Ukweni, S. U.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Eno, A. E.
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences University of Calabar, P.M.B 1115, Nigeria
Seafood consumption has been a way of life to most people especially those that leave in riverine areas, because seafoods are known to contain many nutrients that are essential for healthy living. Consequently, this research therefore seeks to investigate the effect of these nutritive components of Thais coronata on biochemical indices of albino Wistar rats. Fourty five male albino Wister rats weighing between 180-220 g were assigned into 3 groups of fifteen rats each in metabolic cages and were given rat feed and drinking water ad libitum. Two test doses (low dose 7.0 mg protein/ml and high dose 52 mg protein/ml) were selected and administered to two groups of rats orally and daily for six weeks, while a third group of rats served as the control, n = 15. At the expiration of the feeding period, blood samples were obtained from all the rats via cardiac puncture for the analysis of the various biochemical indices. Both the low and high doses of the extract produced significant increases in HDLc (P<0.001) compared with control. k (P<0.001), HCO3- (P<0.01) and Ca2+ (P<0.001) were also significantly increased in the extract treated groups. The extract groups had significant reductions in ALT (P<0.001), ALP (P<0.001), Na+ (P<0.001) and Cl- (P<0.001) compared with control. Also Tc (P<0.001), TG (P<0.001), LDL (P<0.001) and VLDLc (P<0.001) were significantly decreased in the extract treated group. In conclusion seafood consumption is of immense benefit to health because it serves to regulate the lipid profile, electrolytes and enzyme concentrations in blood.
DOI: http://dx.doi.org/10.5281/zenodo.580788
JBródka Publisher
2017-06-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/177
European Journal of Biological Research; Vol 7 No 2 (2017): April-June 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/178
2021-02-26T10:51:37Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170630 2017 eng "
2449-8955
dc
Occurrence and characteristics of the migrating myoelectric complex in ovine gallbladder and its relationships to the small intestinal motility
Romański, Krzysztof Waldemar
Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland
An attempt has been made to identify the migrating motility complex in the ovine gallbladder and to span it with the small-intestinal pattern. For this purpose, four rams underwent surgical implantation of bipolar electrodes into the abomasal antrum, entire small bowel and gallbladder infundibulum, corpus and fundus. The strain gauge force transducer was also mounted in the gallbladder fundus, near the electrode. In the course of chronic experiments, the myoelectrical and motor activity was recorded in fasted and non-fasted rams, with or without feeding. Cyclic myoelectrical and motor activity pattern was found in the gallbladder. It resembled the migrating myoelectric complex present in the small bowel. The gallbladder pattern was well correlated with the intestinal migrating complex. Three or four phases of this pattern could be identified in all gallbladder regions. The most characteristic phase 3-like activity was longer and more intense in the gallbladder fundus as compared with the upper gallbladder regions. In both the small bowel and gallbladder, motility alterations caused by various feeding conditions were comparable. Therefore, the migrating motility complex occurs in the ovine gallbladder, albeit its putative role can be different from that in the small bowel, at least in part.
DOI: http://dx.doi.org/10.5281/zenodo.580794
JBródka Publisher
2017-06-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/178
European Journal of Biological Research; Vol 7 No 2 (2017): April-June 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/179
2021-02-26T10:51:37Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170630 2017 eng "
2449-8955
dc
In silico sequence analysis of predicted beta-amylase 7-like protein in Juglans regia L.
Sevindik, Emre
Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey
Walnut (Juglans regia L.) is a deciduous tree of the Juglandaceae family. Beta-amylase (β-amylase, EC 3.2.1.2) is an enzyme that catalyses hydrolysis of glycosidic bonds in polysaccharides. In this study; sequence, physicochemical, and three-dimensional analyses of predicted β-amylase 7-like protein in Juglans regia using various bioinformatic tools were conducted. The physicochemical properties of the predict β-amylase 7-like protein were analyzed by using ExPASy ProtParam tool that revealed the molecular weight (MW), Isoelectric Points (pI), total number of negatively charged residues (Asp + Glu), total number of positively charged residues (Arg + Lys), instability index, aliphatic index, and GRAVY (Grand Average of Hydropathy) values. Subcellular localization using CELLO v.2.5, putative phosphorylation sites using NetPhos 3.1 server, domain analysis using Pfam, and secondary structure prediction using SOPMA were accom-plished. To predict the 3D structure of the predict β-amylase 7-like protein, homology models were applied using PSIPRED, RAMPAGE, and PyMOL programs. The results of our study provide insight into fundamental characteristics of the predicted β-amylase 7-like protein in Juglans regia.
DOI: http://dx.doi.org/10.5281/zenodo.583137
JBródka Publisher
2017-06-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/179
European Journal of Biological Research; Vol 7 No 2 (2017): April-June 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/180
2021-02-26T10:51:42Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
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Physiological responses to excess boron in wheat cultivars
Metwally, Ashraf M.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt; Biological Sciences Department, Faculty of Science, King Faisal University, Hofuf 31982, Saudi Arabia
El-Shazoly, Rasha M.
Botany Department, Faculty of Science, Assiut University, New Valley, Egypt
Hamada, Afaf M.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
This study investigates the response of two wheat cultivars to boron toxicity stress. Plants were cultivated in sand culture and boron was applied to the culture for 10-day. Symptoms, tiller number, boron concentration, soluble sugars, proteins and other free amino acids than proline were studied. The differences between the cultivars were apparent from higher boron and the chlorosis in tolerant cultivar was about 7% compared to the sensitive one 70%. Tiller number gradual decreased in tolerant-cultivar, while in sensitive one a dramatic reduction was exhibited by increasing boron level in culture media. In most boron levels, although the accumulation of soluble carbohydrates was significantly stimulated in shoot of B-sensitive cultivar (Gemmeza 9; S), there were no appreciable differences in the production of carbohydrates in shoot of B-tolerant cultivar (Sakha 93; T). However, the soluble proteins production did not affect by most boron levels in both cultivars. The presence of boron at various concentrations induced a production of free amino acids in shoots of each of the two test cultivars. Tiller number (yield index) decreased in the two test cultivars and was in range 50-59 and 84-92% less than control plants for tolerant and sensitive cultivar, respectively.
DOI: http://dx.doi.org/10.5281/zenodo.200373
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/180
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/181
2021-02-26T10:51:41Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Nodulation and nitrogen fixation of some wild legumes from differing habitats in Egypt
Abd-Alla, Mohamed H.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
Elenany, Abdelwahab E.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
Mohamed, Taha R.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
Zohri, Manal El
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
Nafady, Ibrahim M.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
This study was devoted to exploring the natural nodulation and nitrogen fixation of wild legumes grown in different Egyptian habitats. These habitats are representative to four phytogeographical regions. Sites that inhabited by Melilotus indicus, Medicago polymorpha, Trifolium resupinatum, Trigonella hamosa and Vicia sativa in each region were selected for study. High nodulation, nitrogen fixation and plant biomass were recorded in plants grown at Nile region and Oases compared with those at Mediterranean region and Sinai. The inhibition in nodulation and potential of nitrogen fixation in legumes at MR and S were attributed to drought and low soil fertility. Differences in species, regions or their interaction have significant effect on nodulation, legheamoglobin, nitroginase activity and biomass of nodules, shoots and roots; the magnitude of effect due to different species was the greatest. Five rhizobial isolates (Sinorhizobium fredii, Rhizobium mesosinicum, Rhizobium daejeonense, Rhizobium huautlense, Rhizobium alamii) recovered from root nodules of the five species were identified by 16S rRNA gene sequence. The indigenous rhizobia of legumes grown at MR and S expected to be exhibit higher tolerance to the existing harsh environmental conditions. These rhizobia can be used as inoculants for crop legumes under unfavorable environmental conditions of agroecosystems or recently reclaimed desert.
DOI: http://dx.doi.org/10.5281/zenodo.224013
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/181
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/182
2021-02-26T10:51:41Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Optimization of lactic acid production by a novel strain, Enterococcus faecalis KY072975 isolated from infants stool in Egypt
Aboseidah, Akram A.
Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt
Rasmey, Abdel-Hamied M.
Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt
Osman, Magdy M.
Dairy Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt
Kamal, Nehal
Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt
Desouky, Salha G.
Department of Botany and Microbiology, Faculty of Science, Suez University, Suez, Egypt
Production of lactic acid using a novel strain of lactic acid bacteria isolated from infants stool was investigated in the present study. Out of ten isolates, a total of five bacterial isolates were found as positive in lactic acid production. The tested bacterial isolate W7 was observed as the potent strain in lactic acid production that exhibited a halo zone of 8 mm. The bacterial isolate W7 was identified phenotypically and genotypically as Enterococcus faecalis and was deposited in GenBank with accession number KY072975. The effect of different process parameters such as initial pH of the medium, incubation temperature, inoculum size and incubation time was also monitored to enhance lactic acid production and resulted in optimum lactic acid value of 0.72 mg/ml. The salted whey was the most applicable fermentation medium for production of lactic acid by Enterococcus faecalis KY072975 and recorded 2.07 ± 0.1 mg/ml.
DOI: http://dx.doi.org/10.5281/zenodo.242164
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/182
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/183
2021-02-26T10:51:41Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Effects of cholecystokinin-octapeptide and cerulein on ovine digestive motility under cholinergic blockade
Romański, Krzysztof W.
Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland
In sheep, contribution of cholinergic system to the control of gastrointestinal motility by cholecystokinin is unknown. Accordingly, in six non-fasted rams chronic experiments were conducted and the myoelectrical activity of abomasal antrum, duodenum and jejunum was recorded before and after injection of atropine (two doses), pirenzepine (two doses), hexamethonium or atropine plus hexamethonium followed or not by injection of three doses of cholecystokinin octapeptide or cerulein. In the course of the experiments performed, the anticholinergic drugs and hormones suppressed spike burst activity both in abomasal antrum and small bowel and inhibited the migrating myoelectric complex and ‘minute rhythm’. When the hormones were injected after cholinergic blockade, they induced longer inhibitory effects than cholinergic blockade alone. In the small bowel, some stimulatory effects were observed as well. The higher dose of pirenzepine and remaining anticholinergics induced rebound excitation in the small bowel, but when followed by cholecystokinin peptide administration, no rebound effect was denoted. Hexamethonium given alone or in combination with atropine followed by cholecystokinin peptide caused stronger inhibitory effect than that of atropine or pirenzepine. It is concluded that cooperation exists between the cholinergic system and cholecystokinin in the control of gastrointestinal motility in sheep and the role of nicotinic mechanisms is greater than that of muscarinic mechanisms.
DOI: http://dx.doi.org/10.5281/zenodo.254010
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/183
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/184
2021-02-26T10:51:40Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Leaf biomass and leaf area equations for three planted trees in Iran
Eslamdoust, Jamshid
Faculty of Natural Resources and Marine Science, Tarbiat Modares University, Jalal Ale Ahmad Highway, P.O.Box: 14115-111, Tehran, Iran
Sohrabi, Hormoz
Faculty of Natural Resources and Marine Science, Tarbiat Modares University, Jalal Ale Ahmad Highway, P.O.Box: 14115-111, Tehran, Iran
Hosseini, Seyed Mohsen
Faculty of Natural Resources and Marine Science, Tarbiat Modares University, Jalal Ale Ahmad Highway, P.O.Box: 14115-111, Tehran, Iran
Naseri, Bahram
Faculty of Natural Resources and Marine Science, Tarbiat Modares University, Jalal Ale Ahmad Highway, P.O.Box: 14115-111, Tehran, Iran
Leaf area (LA) and leaf biomass (LB) are important variables for most physiological, horticultural and agronomic studies involving plant growth, development rate, radiation use efficiency, and water or nutrient use. Measuring these variables need destructive and aggressive sampling. Fortunately, evolving allometric equations can help for low cost and non-destructive estimation of such variables. The aims of this study are Estimate, compare and develop allometric models of LA and LB per tree and per stand for Alnus subcordata (AS), Populus deltoides (PD) and Taxodium distichum (TD) plantations. We selected 12 sample trees in each stand. Leaf Fresh weight of randomly selected branches was weighted in the field. Branch-level LA was modeled as a function of branch diameter (R2 > 0.8) and total fresh weight of LB has been calculated for each sampled tree. For each species, 100 leaves from all canopy directions of trees were randomly selected and transported to the laboratory. At the lab, leaf area has been measured using leaf area meter. Allometric equations were derived using regression analysis. For all species, derived equations showed high accuracy (R2 ranged from 0.837 to 0.947). However, with respect to mean square error, power regression equations (individual leaf area = a(L×W)b and LA or LB = a DBHb) are best models to estimate Individual Leaf Area, LA, and LB of AS, PD, and PD. The highest LAI was in the order of 16.9 > 5.5 > 4.5 for AS, PD, and TD, respectively.
DOI: http://dx.doi.org/10.5281/zenodo.267914
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/184
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/185
2021-02-26T10:51:40Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Moringa oleifera Lam extract attenuates gastric ulcerations in high salt loaded rats
Ofem, O. E.
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria
Ikip, E. E.
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria
Archibong, A. N.
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria
Chukwu, J. A. O.
Department of Physiology, College of Medical Sciences, University of Calabar, Calabar, Nigeria
Moringa oleifera Lam is a plant used extensively both in traditional and orthodox medicine to treat myriad ailments, including gastrointestinal disorders. This study was carried out to investigate the effect of leaf extract of M. oleifera some gastrointestinal function parameters in high salt loaded rats. Acute toxicity study was done using 70 male white mice (18-20 g) were used for the study. They were randomly selected and assigned to 7 cages of 10 animals per cage. Percentage mortalities were converted to probits and plotted against the log10 of the dose of the extract from which the LD50 value was calculated. Fresh leaf extract of M. oleifera was Soxhlet extracted. 24 albino Wistar rats were randomly assigned into 4 main groups of 6 rats each. Fed on normal rat chow, high salt (8% NaCl) diet + 1% NaCl drinking water and/or M. oleifera extract (600 mg/kg bw). The feeding regimens lasted for 42 days. Results obtained revealed that the extract had an LD50 value of 1,872.22 mg/kg from which a test dose of 600 mg/kg was derived for the feeding regimen. The salt fed rats had significantly (p<005) raised basal gastric acid output (9.03 ± 0.17 mmol/L/hr) compared with control (7.27 ± 0.17 mmol/L/hr), but had blunted response to administered histamine and cimetidine, while treatment with the extract enhanced the sensitivity of histamine in high salt loaded rats. Gastric mucus concentration was significantly (p<0.05) higher in the salt untreated group (0.25 ± 0.004 g) compared with other groups. The salt fed untreated group also had significantly (p<0.05) raised gastric ulcers (10.83 ± 0.70) compa-red with other groups, these were reversed following Moringa treatment. In conclusion, Moringa oleifera extract reverses gastric ulcers and blunted histaminergic receptors in high salt fed rats. The mechanism by which high salt increases gastric secretion is independent of the histaminergic mechanism.
DOI: http://dx.doi.org/10.5281/zenodo.290641
JBródka Publisher
2017-03-30 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/185
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/186
2021-02-26T10:51:39Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Antioxidant response of vitamin A during the exposure of blood platelets to electromagnetic radiation generated by LCD monitors - in vitro study
Lewicka, Małgorzata
Department of Epidemiology and Public Health, Military Medical Faculty, Medical University, ul. Żeligowskiego 7/9, 91-752 Łódź, Poland
Zawadzka, Magdalena
Department of Epidemiology and Public Health, Military Medical Faculty, Medical University, ul. Żeligowskiego 7/9, 91-752 Łódź, Poland
Henrykowska, Gabriela
Department of Epidemiology and Public Health, Military Medical Faculty, Medical University, ul. Żeligowskiego 7/9, 91-752 Łódź, Poland
Rutkowski, Maciej
Department of Military Toxicology and Radiological Protection, Military Medical Faculty, Medical University, Łódź, Poland
Pacholski, Krzysztof
Institute of Electrical Engineering Systems, Faculty of Electrical, Electronic, Computer and Control Engineering, Technical University, Łódź, Poland
Buczyński, Andrzej
Department of Epidemiology and Public Health, Military Medical Faculty, Medical University, ul. Żeligowskiego 7/9, 91-752 Łódź, Poland
The article presents the results of in vitro studies aimed at identifying changes in activity of the enzyme superoxide dismutase (SOD-1) as a parameter of oxidative stress and protective antioxidant role of vitamin A during the exposure of blood platelets to electromagnetic radiation (EMR) generated by LCD monitors. Blood platelets were exposed to an electromagnetic radiation for 30 min. and 60 min. generated by monitors, which is characterized by parameters: 1 kHz frequency and 220 V/m intensity. The enzymatic activity of SOD-1 increases significantly compared to control values after 30 min. of exposure to EMR (from 2523.39 U/g protein to 3896.15 U/g protein), and decreases after 60 min (to 2846.58 U/g protein). A significant decrease in enzyme activity after the addition of vitamin A was noticed (to 1569.54 U/g protein). In samples exposed for 30 min. the SOD activity was significantly increased by addition of vitamin A and decreases after 60 min. Changes in enzymatic activity of SOD-1 dependent on exposure time and application of vitamin A suggest an important preventive role of vitamin A to protect against the effects of EMR which we are exposed to in everyday life.
DOI: http://dx.doi.org/10.5281/zenodo.321600
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/186
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/187
2021-02-26T10:51:39Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"170331 2017 eng "
2449-8955
dc
Antioxidant potentialities of some strains belonging to endophytic, entomopathogenic and saprophytic fungi
Zohri, A. A.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Moharram, A. M.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Abd El-Ghani, O. A.
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Antioxidants have recently become the topic of interest as radical scavengers, which inhibit the free radical mediated processes. This study was carried out to investigate the antioxidant activity of 100 fungal strains (26 endophytes, 42 entomopathogens and 32 saprophytes). Three different assays (reducing power, total phenolic contents and flavonoid contents) were determined and used to evaluate the antioxidant potential of the fungal ethanolic extracts. The results revealed that all fungal strains under study showed antioxidant activity up to varying extent. A total of 21, 35 and 19 out of the tested endophytic, entomopathogenic and saprophytic strains, respectively, had a reducing power activity. High reducing power activities (≥ 0.6 mg/ml) were recorded by 9, 20 and 14 strains of the three tested groups, respectively. All tested strains have the ability to produce phenolic compounds with levels ranged from 0.92 to 63.44 mg/ml. The highest levels of total phenolic contents (≥ 40 mg/ml) observed in the extract of 12, 28 and 18 strains of endophytes, entomopathogens and saprophytes, respectively. Finally, all tested strains produced flavonoids with levels of 0.166 to 68.806 mg/ml. The highest flavonoid producers (formed ≥ 35 mg/ml) were only one strain of each of the endophytic and entomopathogenic fungi and three strains of saprophytic fungal group. The obtained results suggest that the tested strains, especially those of endophytes, had the potentiality as sources of strong natural and safe antioxidants for application in food and cosmetics industries.
DOI: http://dx.doi.org/10.5281/zenodo.398829
JBródka Publisher
2017-03-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/187
European Journal of Biological Research; Vol 7 No 1 (2017): January-March 2017
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/192
2021-02-26T10:51:42Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"161215 2016 eng "
2449-8955
dc
Bioconversion of plant wastes to β-carotene by Rhodotorula glutinis KU550702
Bagy, Magdy Mohamed Khalil
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Abd-Alla, Mohamed Hemida
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Nafady, Nivien Allam
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Morsy, Fatthy Mohamed
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Mahmoud, Ghada Abd-Elmonsef
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Microbial synthesis of β-carotene has gained more interest as an alternative to synthetic β-carotene due to easy extraction and high yield. The vitamin microbial production is mainly dependent on culture conditions and the medium compositions. In this study, the β-carotene production by the Rhodo-torula glutinis ASU6 (KU550702) was evaluated under different growth conditions and nutrient composition. Different agro-renewable wastes were tested as carbon source for R. glutinis to obtain maximum amount of β-carotene. Meanwhile, it is clear that R. glutinis could grow well on acid extract of onion peels and produced large amount of β-carotene. Initial statistical screening using a Plackett-Burman design showed temperature, incubation time, fermentation type, non-treated onion waste, KH2PO4 and L-asparagine as significantly, influencing β-carotene production. Response surface methodology was applied to determine the mutual interactions between these parameters and optimal levels for β-carotene production. The maximum value of β-carotene production was 204.29 mg/l (7.5-fold) of value observed as central point of the central composite design. All the experimental data are in good agreement with predicted ones, confirming the responsibility of the proposed empirical model in describing β-carotene production by R. glutinis. In the whole, the outcomes of this study support the exploitation of onion peels through microbial fermentation for β-carotene production.
JBródka Publisher
2016-12-30 00:00:00
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/192
European Journal of Biological Research; Vol 6 No 4 (2016): October-December 2016
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/205
2021-02-26T10:51:13Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190927 2019 eng "
2449-8955
dc
In vitro evaluation of antimicrobial activities from aqueous and methanolic extracts of cyanobacteria
Safari, Moein
Department of Biology, Faculty of Basic Science, Ilam University, Ilam, Iran
Ahmady-Asbchin, Salman
Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran
Zamanifar, Pantea
Department of Biology, Faculty of Basic Science, Islamic Azad University, Varamin-Pishva branch, Tehran, Iran
In this present study, antimicrobial activities of aqueous and methanolic extracts of cyanobacteria against some of fungi and pathogenic bacteria were investigated. Cyanobacteria strains Fischerella ambigua ISC67 and Schizothrix vaginata ISC108 were cultured in BG-11 medium. Extraction was performed by adding the solvent to cyanobacterial biomass and then filtering and drying of the mixture. The antimicrobial activity was evaluated by disc diffusion method and broth microdilution method was applied to determine the minimum inhibitory concentration. The results show that the aqueous and methanolic extracts of F. ambigua has a significant antimicrobial effect while, the tested extracts of S. vaginata was no significant antibacterial and antifungal activity. Highest antibacterial activity from aqueous extract of F. ambigua was against S. aureus (PTCC 1112) which the average zone diameter around it was 33.33 mm. The antibacterial effect of aqueous extracts against Gram-positive bacteria was more than Gram-negative bacteria significantly. Antifungal activity showed that methanolic extract of F. ambigua have significant antifungal activity. Minimum inhibitory concentration of active extract against most tested bacterial and fungal was 125 mg/ml. The present study has proved that the aqueous and methanolic extracts of F. ambigua possessed strong antibacterial and antifungal properties against the pathogenic microorganism. Therefore, cyanobacteria can be a rich source for natural products with antimicrobial activity.
DOI: http://dx.doi.org/10.5281/zenodo.3463632
JBródka Publisher
2019-07-18 17:43:17
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/205
European Journal of Biological Research; Vol 9 No 3 (2019): July-September 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/206
2021-02-26T10:51:13Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190915 2019 eng "
2449-8955
dc
Detection of carbapenem resistant bacteria (CRB) in Egypt
Abd El-Malek, Fady Fady
Department of Microbiology, Faculty of Science, Alexandria University, Egypt
The emergence of resistant bacteria has become a worldwide threat. Multidrug resistant bacteria are globally spread. Several studies were performed to detect new resistant organisms and also the genes which are responsible for their resistance. Carbapenem resistance is considered the most dangerous resistance. In this study, we detect the presence of carbapenem resistant bacteria (CRB) in Egypt. This may cause un-treatable epidemic if its organization is neglected. This study distinguished the pathogens that are carbapenemase producing due to the presence of bla-NDM gene. The results detected the presence of CRB stains such as Klebsiella sp., Pseudomonas sp., Citrobacter sp., Enterobacter sp., Acinetobacter sp. and E. coli. As a result from this study, it is now proved that there are CRB in Egypt, thus it must be given a great consideration and must be managed.
DOI: http://dx.doi.org/10.5281/zenodo.3408764
JBródka Publisher
2019-07-18 17:43:17
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/206
European Journal of Biological Research; Vol 9 No 3 (2019): July-September 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/207
2021-02-26T10:51:15Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"190618 2019 eng "
2449-8955
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Efficacy of octenidine against Pseudomonas aeruginosa strains
Karpiński, Tomasz M.
Department of Medical Microbiology, Poznań University of Medical Sciences, Wieniawskiego 3, 61-712 Poznań, Poland https://orcid.org/0000-0001-6599-9204
Pseudomonas aeruginosa is a Gram-negative bacterium causing skin and soft tissue infections, complicated urinary tract infections, blood infections, and nosocomial (hospital-acquired) infections. One of the most often used antiseptics in the skin and soft tissue infections is octenidine dihydrochloride. The aim of this study was an evaluation of octenidine activity against strains of P. aeruginosa. Additionally, were compared two staining methods (TTC and MTT) for confirmation of bacterial growth. The study involved eight strains of P. aeruginosa. In order to determine the minimum inhibitory concentration (MIC) of octenidine, the microdilution method was used. For bacterial growth detection was used staining method with 2,3,5-triphenyl-tetrazolium chloride (TTC) and with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). In the study has been demonstrated the excellent activity of octenidine against all strains of Pseudomonas aeruginosa. For all tested strains, MICs of octenidine were 0.00039% or 0.00078%, what is equivalent to 3.9 µg/ml and 7.8 µg/ml, respectively. In the study, test with MTT for three strains was more sensitive than a test with TTC. Concluding, octenidine is an antiseptic with high efficacy against Pseudomonas aeruginosa strains. Simultaneously, it was stated that a test with MTT is more sensitive than study with TTC.
DOI: http://dx.doi.org/10.5281/zenodo.3339499
JBródka Publisher
2019-07-18 17:43:17
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/207
European Journal of Biological Research; Vol 9 No 3 (2019): July-September 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/217
2021-02-26T10:51:12Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"191110 2019 eng "
2449-8955
dc
Soil mites (Acari) of natural areas of a former military training field in Olsztyn (Poland)
Zduniak, Milena
Department of Systematic Zoology, Faculty of Biology AMU, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Błoszyk, Jerzy
Department of General Zoology, Faculty of Biology AMU, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland; The Natural History Collections, Faculty of Biology AMU, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Nowak, Maciej
Laboratory of Biological Spatial Information, Faculty of Biology, AMU, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Napierała, Agnieszka
Department of General Zoology, Faculty of Biology AMU in Poznań, ul. Umultowska 89, 61-614 Poznań,
Due to restricted public access to military training fields such areas are quite interesting places for conducting faunistic research that would be simply impossible in other terrains. The area examined in the present study was the former military training field in Olsztyn, with the adjacent Lasek Pieczewski and the valley of Skanda Lake. The major aim of the study was to evaluate the current state of the environment in the terrain of the former military training field and the adjacent areas. In this study mites from the suborder Uropodina and cohort Labidostommatina (Acari: Mesostigmata et Prostigmata) were used as a bioindicators. These mites are useful for this purpose because of their specific habitat preferences. The community of mites in the area under scrutiny contained 23 taxa, comparing to 34 species found in whole voivoideship, which is 68% of local species biodiversity. Oodinychus ovalis turned out to be the most numerous species (the specimens of this species constituted almost 38% of the whole community and the frequency per sample was 55%). The other quite numerous species found in the examined area were Janetiella pulchella and Oodinychus karawaiewi, which constituted over 30% of the whole community. Taking into account the number of species and their habitat preferences two most valuable areas were found: southern part of Lasek Pieczewski and Skanda Lake valley.
DOI: http://dx.doi.org/10.5281/zenodo.3534449
JBródka Publisher
2019-11-04 22:53:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/217
European Journal of Biological Research; Vol 9 No 4 (2019): October-December 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/230
2021-02-26T10:51:11Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"191210 2019 eng "
2449-8955
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Treatment of facial aging with calcium hydroxyapatite - filling and lifting concept
da Cunha, Marisa Gonzaga
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
da Cunha, Ana Lúcia Gonzaga
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
Gonzaga, Meire
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
da Veiga, Glaucia Luciano
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
Alves, Beatriz da Costa Aguiar
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
Fonseca, Fernando Luiz A.
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
Filho, Carlos A. Machado
Centro Universitário Saúde ABC/Faculdade de Medicina do ABC, FMABC, Santo André, São Paulo, Brasil
The face is organized in five different layers (skin, muscles, supportive ligaments, fat pads and bones), which are continuous and interconnected with each other from the scalp to the neck. As a result of aging, changes occur in all its tissues and structures, triggering a cascade of effects in the adjacent areas. Actually, the more it is known about the loss of volume, the better it is to establish the target spots for volume replacement, with consequent more natural- and harmonious-looking outcomes. However, the skin is the outer envelope of the face, which, in the natural process of aging, has a lower capacity to completely accommodate any underlying volume loss or displacement. The result is the formation of creases and folds due to skin sagging. To describe the effects of the injection of calcium hydroxyapatite (CaHA) targeting two objectives at the same time: the restoration of volume loss to compensate the changes in the tridimensional structure and the improvement in skin texture caused by the stimulus to collagen synthesis, thus reducing the sagging of soft tissues envelope. Owing to the mechanism of action of CaHA, its application can be done in two differentiated and little invasive ways so that these objectives can be reached simultaneously. This technique can provide safe, natural and long-lasting rejuvenating effects.
DOI: http://dx.doi.org/10.5281/zenodo.3569835
JBródka Publisher
2019-11-04 22:53:11
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/230
European Journal of Biological Research; Vol 9 No 4 (2019): October-December 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/234
2021-02-26T10:51:11Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"191220 2019 eng "
2449-8955
dc
Ameliorating effect of quercetin against UV radiation-induced damage in Drosophila melanogaster
Majumder, Susmita
Post Graduate Department of Zoology, Asutosh College, Kolkata, West Bengal, India
Bandyopadhyay, Mohna
Department of Dermatology, University of Pittsburgh, Pennsylvania, USA
Pal, Sandip
Department of Zoology, Barrackpore Rastraguru Surendranath College, Barrackpore, West Bengal, India
Mukhopadhyay, Dalia
Post Graduate Department of Zoology, Asutosh College, Kolkata, West Bengal, India
Quercetin is a plant flavonoid found in various fruits, leaves such as tea, vegetables and has been extensively studied due to its antioxidative, anticancer, anti-inflammatory and anti-neurodegenarative effects. UV radiation is harmful for human being as it may cause several complications such as skin cancer. Fruit fly (Drosophila sp.) has long been used as an arthropod model for genetics related studies. In the present study, the protective effect of quercetin is evaluated against UV-C radiation induced damage using Drosophila melanogaster. Pre-treatment with quercetin (10 µM) recovered the shortened lifespan caused by UV radiation and has also increased eclosion rate and the dose of quercetin is lower than the previously reported doses of other flavonoids. Flies subjected to moderate dose of UV radiation showed distinct abnormal characters such as incomplete abdominal pigmentation, curly wings or outstretched wings, whereas quercetin pretreatment showed no such abnormal characters or mutant phenotypes. There is a considerable amount of change in the eclosed adult fly size, pupal size and pupal migration distance as well. Gel electrophoresis study of salivary gland DNA of D. melanogaster demonstrates the efficacy of quercetin in conferring protection to DNA against UV radiation-induced damage. Therefore, it can be concluded that quercetin may act as an effective protective agent against UV radiation-induced damage.
DOI: http://dx.doi.org/10.5281/zenodo.3588543
JBródka Publisher
2019-11-04 22:53:11
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/234
European Journal of Biological Research; Vol 9 No 4 (2019): October-December 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/237
2021-02-26T10:51:11Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200129 2020 eng "
2449-8955
dc
Pseudomonas species from cattle dung producing extended spectrum and metallo beta-lactamases
Falodun, Olutayo Israel
Department of Microbiology, University of Ibadan, Ibadan, Nigeria
Musa, Isaiah Baba
Department of Microbiology, University of Ibadan, Ibadan, Nigeria
Indiscriminate use of antibiotics in livestock contributes to emergence of antimicrobial resistance in pathogens co-habiting the gastro-intestinal tract of animals. This study was to determine the Extended Spectrum Beta-Lactamase (ESBL) and Metallo-Beta-Lactamase (MBL) production in Pseudomonas species from cattle fecal samples. Cattle dungs were collected from the University of Ibadan Cattle Ranch and the Pseudomonas species isolated using Pseudomonas Base Agar with Pseudomonas CN Selective Supplement were identified using standard tests. Phenotypic detection of ESBL and MBL was by double disk synergy test and Ethylene Di-amine Tetra Acetic Acid Combined Disk Test respectively. Antibiotics susceptibility tests was done using the disc diffusion technique against ten antibiotics. A total of 144 Pseudomonas species were isolated and identified as P. aeruginosa (71.5%), P. fluorescens (19.4%) and P. stutzeri (9.1%) and 19 (37.1%) produced ESBL including P. aeruginosa (15), P. fluorescens (2) and P. stutzeri (2) while, one (6.7%) ESBL P. aeruginosa produced MBL. All the ESBL producers were resistant to cefotaxime and trimethoprim; resistance of P. aeruginosa to ciprofloxacin was 93.3% and to ceftazidime was 80.0%, while it was 13.3% (colistin) and 6.7% (imipenem). The ESBL producing P. fluorescens were resistant to ceftazidime, ciprofloxacin and trimethoprim, likewise, the ESBL producing P. stutzeri showed resistance to gentamicin, ciprofloxacin and trimethoprim. The production of ESBL and MBL observed among the Pseudomonas species in this study with high level of resistance to some antibiotics portend public health risk, hence a need for caution in the use of antibiotics in animal husbandry.
DOI: http://dx.doi.org/10.5281/zenodo.3630802
JBródka Publisher
2020-01-29 23:16:12
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/237
European Journal of Biological Research; Vol 10 No 1 (2020): January-March 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/238
2021-02-26T10:51:10Z
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driver
nmb a2200000Iu 4500
"200414 2020 eng "
2449-8955
dc
Antifungal activity of Myrtus communis and Zygophyllum album extracts against human pathogenic fungi
Belmimoun, Asmaa
Laboratory of Research, Bioconversion, Engineering Microbiology and Health Safety, University of Mascara, Algeria
Meddah, B.
Laboratory of Research, Bioconversion, Engineering Microbiology and Health Safety, University of Mascara, Algeria
Meddah, A. T. T.
Laboratory of Research, Bioconversion, Engineering Microbiology and Health Safety, University of Mascara, Algeria
Gabaldon, J.
Food Technology Science Department, Católica San Antonio University, Murcia, Spain
Sonnet, P.
Laboratory of Glucides, Team Thera., FRE-CNRS 3517, Faculty of Pharmacy, University of Picardie, Amiens, France
Fungal infections have been increasing in recent years due to a growing number of high-risk patients, particularly immunocompromised hosts. Currently, medicinal plants are known for their properties due to their essential oils and phenolic compounds. They have been empirically used as antimicrobial agents. So the composition of the phenolic extracts and essential oils of Myrtus communis and Zygophyllum album and their antifungal activity on Candida albicans, Aspergillus fumigatus fungal strains were studied. In this fact, essential oils from the aerial parts of the plant were obtained by hydrodistillation and analyzed by GC and GC-MS, for the phenolic extracts, several extraction methods with a preliminary phytochemical study were applied. The oils showed high contents of α-pinene and cineol for M. communis and verbenone and caryophyllene for the Z. album. The MIC and minimal lethal concentration were used to evaluate the antifungal activity against Candida and Aspergillus strains. Results showed that M. communis and Z. album essential oil and phenolic extracts exhibited a significant activity against clinically relevant fungi, a significant antifungal activity of the two extracts studied (MCA and ZAM) was observed on C. albicans of these, two extracts, MCA was found to be most active with an MFC value of 25 mg/ml versus 100 mg/ml for ZAM. Nevertheless, the essential oils exhibited stronger antifungal activity than the phenolic extracts. The present study indicates that the two medicinal plants have considerable antifungal activity, deserving further investigation for clinical applications.
DOI: http://dx.doi.org/10.5281/zenodo.3751202
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/238
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/240
2021-02-26T10:51:12Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"191104 2019 eng "
2449-8955
dc
Lac, Kerria lacca rearing on Flemingia macrophylla with NPK fertilizer: impact on plant growth, lac yield, and lac parasitisation
Kumar, Arvind
Forest Protection Division, Forest Research Institute, Dehradun-248006 (Uttarakhand), India
Rani, Manju
Institute of Forest Productivity, Ranchi (Jharkhand), India
Lac insect, Kerria lacca Kerr. is the only commercial lac producing insect in the world. This tiny insect reared commercially on many specific host plants. Lac product is a natural resin of outstanding properties which is utilized in many products worldwide. Lac insects get settled on the host tree and take their nutrition continuously from the same part. Hence, additional fertilizer application becomes most important component for sustainable host plant growth and lac cultivation. Therefore, to determine the effect of chemical fertilizers (NPK) on Flemingia macrophylla and lac productivity, present study has been conducted. The result revealed that chemical fertilizer combination N15:P5:K5 was found to be the best for Flemingia macrophylla growth which gives best lac yield and least pest infestation on Kerria lacca. The correlation study has also proved that NPK has positively influences on plant growth and lac productivity.
DOI: http://dx.doi.org/10.5281/zenodo.3551585
JBródka Publisher
2019-11-04 22:53:11
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/240
European Journal of Biological Research; Vol 9 No 4 (2019): October-December 2019
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/243
2021-02-26T10:51:10Z
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driver
nmb a2200000Iu 4500
"200315 2020 eng "
2449-8955
dc
Production and optimization of polyhydroxybutyrate (PHB) from Bacillus megaterium as biodegradable plastic
Ahmady-Asbchin, Salman
Department of Molecular and Cell Biology, Faculty of Basic Science, University of Mazandaran, Babolsar, Iran
Rezaee, Hassan
Department of Biology, Faculty of Basic Science, Ilam University, Ilam, Iran
Safari, Moein
Department of Biology, Faculty of Basic Science, Ilam University, Ilam, Iran
Zamanifar, Pantea
Department of Biology, Faculty of Basic Science, Islamic Azad University, Varamin-Pishva branch, Tehran, Iran
Siyamiyan, Davood
Department of Biology, Faculty of Basic Science, Islamic Azad University, Tonekabonbranch, Mazandaran, Iran
Among biodegradable plastics polyhydroxy alkanate and its polymers have received more attention than other biodegradable polymers because of their complete degradability, flexibility, water resistance and also the ease of production process. Polyhydroxybutyrate is one of the types of polyhydroxy alkanates that is seen as a storage granule in many microorganisms. In this study, Bacillus megaterium was prepared from Iranian microbial collection. Glucose and yeast extract were used as the main components of the medium in seed media 9 and 2.5 g/l and in fermentation medium 30 and 7.5 g/l respectively. GC-MASS and FTIR were used to identify the PHB produced. The results showed that the highest amount of biomass (0.221 g/l) and PHB (0.080 g/l) were obtained with glucose at 37°C and shaker speed of 150 rpm for 72 h incubation. The results of GC MASS and FTIR showed the production of PHB by Bacillus under investigation. Based on the mean of data on total cell growth conditions, the rate of cell biomass and PHB production in B. megaterium were 0.0869 and 0.0171 respectively. According to the results of the experiments, temperature had the greatest effect on biomass production and PHB production. The bioplastics produced by microbes are also highly degradable in the environment, and due to their specific chemical structure, they have been widely used in various fields of the food, pharmaceutical and chemical industries and are likely to replace today's plastics in the near future.
DOI: http://dx.doi.org/10.5281/zenodo.3711400
JBródka Publisher
2020-01-29 23:16:12
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/243
European Journal of Biological Research; Vol 10 No 1 (2020): January-March 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/247
2021-02-26T10:51:08Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200515 2020 eng "
2449-8955
dc
Screening of yeasts obtained from different fermented foods for their ability to produce pectinase
Afolabi, Folake Titilayo
Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria
Shitta, Yusuf O.
Department of Microbiology, Faculty of Science, University of Ibadan, Nigeria
In the present study, Citrus pectin was used for the production of pectinase enzyme by yeast isolates using submerged fermentation. Fifty yeasts were isolated from different fermented foods and screened for their producing ability. Candida sp. OG2 and Candida tropicalis strain AUMC 10275 were the yeast isolates with the best potential of pectinase production. Fermentation parameters such as incubation period, pH, temperature, carbon and nitrogen source were optimized under submerged fermentation. The optimal conditions for pectinase production were found to be incubation time 48 hours, pH 6.0 and temperature 40°C. Citrus pectin best induced the production of pectinase while yeast extract/peptone (1:1) was the best source of nitrogen. Pectinase produced by Candida tropicalis strain AUMC 10275 was purified at 4.00 folds with a specific activity of 63.99 U/ml. The yeasts obtained from fermented foods have the ability to produce pectinase enzyme under optimized conditions and can be used for industrial purposes.
DOI: http://dx.doi.org/10.5281/zenodo.3828648
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/247
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/251
2021-02-26T10:51:10Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200331 2020 eng "
2449-8955
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Phycoremediation of water contaminated with arsenic (As), cadmium (Cd) and lead (Pb) from a mining site in Minna, Nigeria
Abioye, Olabisi Peter
Department of Microbiology, Federal University of Technology, P.M.B. 65, Niger State, Nigeria
Ezugwu, Basil Utazi
Department of Microbiology, Federal University of Technology, P.M.B. 65, Niger State, Nigeria
Aransiola, Sesan Abiodun
Bioresources Development Centre, National Biotechnology Development Agency, P.M.B. 3524 Onipanu, Ogbomoso, Nigeria
Ojeba, Musa Innocent
Department of Microbiology, Federal University of Technology, P.M.B. 65, Niger State, Nigeria
This study was designed to remediate water contaminated with heavy metals (arsenic, cadmium and lead) using two green macroalgal species, Spirogyra and Cladophora. The results obtained from this study indicate that both macroalgae can be employed to adsorb and detoxify any of the three heavy metals from aqueous solution. However, it was also discovered from the study that Cladophora adsorbed and detoxified more of the cadmium and lead than arsenic as the organism had removal efficiency for cadmium and lead as 88.78% and 94.85% respectively meanwhile for arsenic it was only 23.10%. On the otherhand however, Spirogyra adsorbed more of arsenic than cadmium and lead as the organism had a record of 82.76% of arsenic compared to the 28.97% and 47.43%absorption forcadmium and lead respectively. It is therefore concluded based on the results of the present study that reclamation and reuse of water from public or industrial wastewater, or even from water contaminated as a result of precious metal mining is a huge possibility through the application of phycoremediation, using different species of micro and macroalgae.
DOI: http://dx.doi.org/10.5281/zenodo.3735302
JBródka Publisher
2020-01-29 23:16:12
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/251
European Journal of Biological Research; Vol 10 No 1 (2020): January-March 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/256
2021-02-26T10:51:11Z
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nmb a2200000Iu 4500
"200303 2020 eng "
2449-8955
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An overview of floral and faunal diversity in and around Barrackpore Rastraguru Surendranath College Campuses, West Bengal, India
Ray, Monojit
Department of Chemistry, Barrackpore Rastraguru Surendranath College, Barrackpore, West Bengal, India
Pal, Sandip
Department of Zoology, Barrackpore Rastraguru Surendranath College, Barrackpore, West Bengal, India
The present survey based study involves the qualitative diversity of flora and fauna. The diversity assessment was carried out at two campuses of Barrackpore Rastraguru Surendranath College. This extensive study reveals the presence of 256 floral species and 165 faunal species in and around college campuses. The huge faunal diversity is mainly due to high level of floral diversity, which establishes the area as resource-rich habitat with promising reservoir of species. This is the very first effort in exploring the natural wealth of Barrackpore Rastraguru Surendranath College campuses.
DOI: http://dx.doi.org/10.5281/zenodo.3695917
JBródka Publisher
2020-01-29 23:16:12
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/256
European Journal of Biological Research; Vol 10 No 1 (2020): January-March 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/257
2021-02-26T10:51:09Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200423 2020 eng "
2449-8955
dc
Comparative pollen morphology of Calycanthaceae for their taxonomic implication
Paudel, Niroj
Department of Applied Plant Science, Kangwon National University, Chuncheon 24341, South Korea
Heo, Kweon
Department of Applied Plant Science, Kangwon National University, Chuncheon 24341, South Korea
The pollen morphology of four species of Calycanthaceae is described based on the observation of scanning electron microscopy and light microscopy. All pollens are monad, large grain category. The pollen grain was elliptic with disulcate. Surface ornamentation is smooth. Pollen grains of each species are from the genus Sinocalycanthus, Calycanthus, Chimonanthus and Idiospermum. The basic shape of the pollen grains is elliptical. The pollen is spheroid in Calycanthaceae except in Idiospermum, which represent boat-shape. The circular shape was in polar views in Chimonanthus but equatorial shape in Sinocalycanthus. Idiospermum and Chimonanthus were smooth exine with micro-perforation but rugose exine in Sinocalycanthus and Chimonanthus.
DOI: http://dx.doi.org/10.5281/zenodo.3763335
JBródka Publisher
2020-04-14 14:28:50
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/257
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/258
2021-02-26T10:51:07Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200520 2020 eng "
2449-8955
dc
Leiodinychus orbicularis (C.L. Koch, 1839) in bat boxes in Poland
Błoszyk, Jerzy
Department of General Zoology, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland; Natural History Collections, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Rutkowski, Tomasz
Natural History Collections, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Wojtaszyn, Grzegorz
Polish Society for Nature Conservation "Salamandra", ul. Stolarska 7/3, 60-788 Poznań, Poland
Książkiewicz-Parulska, Zofia
Department of General Zoology, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Zacharyasiewicz, Michał
Department of General Zoology, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Napierała, Agnieszka
Department of General Zoology, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
The presented studies are the first one on the occurrence of mites in bat boxes and focuses on Uropodina (Acari: Mesostigmata). Investigation was carried out in Western Poland in October 2015. Guano was collected from 58 bat boxes occupied by 10 species of bats. Excrements from particular bat boxes were placed separately in string bags and transported to the laboratory. The extracted fauna was preserved in 75% ethanol and mites were identified with stereoscopic light microscope. The study revealed only one species of Uropodina mite on bat guano in the studied bat boxes, namely Leiodinychus orbicularis (C.L. Koch, 1839).
DOI: http://dx.doi.org/10.5281/zenodo.3835917
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/258
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/259
2021-02-26T10:51:08Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200429 2020 eng "
2449-8955
dc
The first report of the coproduction of CMY-16 and ArmA 16S rRNA methylases in carbapenemase-ESBL producing Escherichia coli isolates
Meziani, Meriem Meriem
Laboratory of Applied Biochemistry, Department of Microbiology, Faculty of Nature and Life Sciences, University of Mentouri Brothers, Constantine1, Algeria
Benlabed, Kaddour
Laboratory of Bacteriology, CHU of Constantine, Algeria
Bogaerts, Pierre
National Belgian Reference Center for Antimicrobial Resistance in Gram-negative Bacteria and Microbiology Laboratory of the University Hospital CHU Mont-Godinne, Catholic University of Louvain (UCL), Av Dr. Gaston Therasse1, 5530 Yvoir, Namur, Belgium
Glupczynski, Youri
National Belgian Reference Center for Antimicrobial Resistance in Gram-negative Bacteria and Microbiology Laboratory of the University Hospital CHU Mont-Godinne, Catholic University of Louvain (UCL), Av Dr. Gaston Therasse1, 5530 Yvoir, Namur, Belgium
The main aim of this work was to assess the occurrence and to characterize AmpC genes and to investigate the co-existence of 16S rRNA methylases and carbapenemases genes among the ESBL producing Escherichia coli strains. 180 Escherichia coli clinical strains were collected from the university hospital of Constantine located in the eastern part of Algeria. 42 ESBL-producers were phenotypically identified and also confirmed genotypically able to produce CTX-M-15 [n=33], CTX-M-1 [n=5], CTX-M-14 [n=1], SHV-2 [n=1], and two strains have been revealed producing the blaOXA-48 genes associated with blaTEM-1. Among the ESBL-producing strains three expressed additionally an AmpC phenotype which corresponded to the carriage of a blaCMY gene shown by sequencing to correspond to CMY-2 (1 isolate) CMY-16 (2 isolates). The two E. coli isolates produce CMY-16 that belonged to phylogroup D while the single CMY-2 producing isolate belonged to phylogroup C. Antibiotic resistance of the aminoglycoside family by production of 16S rRNA methylases was detected by an end-point multiplex PCR assay which concerns genes coding for different 16S rRNA methylases (rmtD, rmtA, rmtB, armA, npmA, and rmtC). An armA gene was identified in 2 strains. This study shows for the first time the co-existance of CMY-16 and armA genes with blaTEM-1 and blaOXA-48 producing E. coli strains.
DOI: http://dx.doi.org/10.5281/zenodo.3776651
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/259
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/260
2021-02-26T10:51:07Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200516 2020 eng "
2449-8955
dc
Peppermint (Mentha piperita L.) essential oil as a potent anti-inflammatory, wound healing and anti-nociceptive drug
Kehili, Sarah
Laboratory of Research on Bio-Active Products and Valorization of Biomass, Department of Chemistry, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
Boukhatem, Mohamed Nadjib
Department of Biology and Cell Physiology, Faculty of Life and Natural Sciences, University - Saad Dahab - Blida 1, B.P. 270, Blida, Algeria; Research Laboratory of “Ethnobotany and Natural Products”, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
Belkadi, Asma
Laboratory of Pharmacology and Toxicology, Research and Development Center, SAIDAL Pharmaceutical, Gué de Constantine, Algiers, Algeria
Ferhat, Mohamed Amine
Laboratory of Research on Bio-Active Products and Valorization of Biomass, Department of Chemistry, Ecole Normale Superieure, B.P. 92, Kouba, Algiers, Algeria
Setzer, William N.
Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA; Aromatic Plant Research Center, 230 N 1200 E, Suite 100, Lehi, UT 84043, USA
The present investigation was designed to study the chemical composition of Algerian peppermint essential oil (PEO) as well as the in vitro and in vivo anti-inflammatory, wound-healing and anti-nociceptive properties. Twenty-three compounds were identified in the PEO with the main chemical component as menthol (53.29%). Also, PEO showed a high content of oxygenated monoterpene compounds (92.75%). Topical application of PEO at doses of 200 and 20 µL/kg significantly reduced the acute ear edema in 38.09% and 36.50, respectively. Histological observation confirmed that PEO inhibited the skin inflammatory response. In-vivo wound healing activity of the cream prepared from PEO (0.5% w/w) was assessed by circular excision wound model followed by histological examination. The topical administration of PEO cream showed a significant decrease of unhealed wound area rate between the 6th (1.67±0.14 mm2) and the 9th (0.49±0.22 mm2) days of treatment when compared with the vehicle (2.32±0.77 mm2; p<0.05) and Madecassol® 0.1% creams (2.23±0.35 mm2; p<0.05). The PEO reduced nociceptive behavior at all doses tested in the acetic acid-induced nociception test (p<0.05). These findings support the anti-inflammatory, wound-healing and analgesic properties of PEO. We suggest that PEO is a promising candidate for use in skin care products with anti-inflammatory and wound-healing properties.
DOI: http://dx.doi.org/10.5281/zenodo.3831042
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/260
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/263
2021-02-26T10:51:09Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200423 2020 eng "
2449-8955
dc
Enhance productivity and net economic return by intercropping sunflower (Helianthus annus L.) with common beans (Phaseolus vulgaris L.) under drip irrigation
Hamd-Alla, Wael
Agricultural Research CenterDepartment of Crop Intensification Research, Field Crops Research Institute, Agricultural Research Center, Giza, Egypt
Ahmed, Nagwa
Agricultural Research CenterDepartment of Crop Intensification Research, Field Crops Research Institute, Agricultural Research Center, Giza, Egypt
Hefzy, Mohamed
Department of Water Requirement and Field Irrigation Research, Soils Water and Environment Research Institution, Agricultural Research Center, Giza, Egypt
Intercropping represents ways of maximizing water use efficiency (WUE) for higher yields per unit of irrigation water applied. The field experiments were carried out at the Experimental Farm of Arab El- Awammer Research, Station, Assiut during the two successive growing summer seasons of 2017 and 2018, to study the effect of different irrigation regimes (120, 100 and 80% ETo) and intercropping systems (sole sunflower, sole common bean and intercropping sunflower and common bean) for enhanced productivity and net economic return under drip irrigation. Irrigation with 120% ETo treatment gave higher yield and its compounds and oil % compared to 100 and 80% ETo treatments for sunflower and common bean. The highest stem, head diameters and 100-seed weight and seed and oil yield produced with sole sunflower as compared with intercropping of sunflower with common bean which had the lowest values in both growing seasons. The highest values of IWUE (0.723 and 0.704 kg/m3) were obtained at intercropping under irrigation with 100% ETo. Values of land equivalent ratio of various intercropping systems were larger than one in the intercropping systems. Sunflower + common bean cropping system produced higher values of net return than sole sunflower and sole common bean. The highest net return (2709 US$/ha) were obtained when irrigated sunflower + common bean intercropping system with 120% ETo in the second season while the lowest net return (234 US$/ha) were obtained when irrigated sole sunflower with 80% ETo treatment in the first season.
DOI: http://dx.doi.org/10.5281/zenodo.3763279
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/263
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
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oai:ojs2.journals.tmkarpinski.com:article/266
2021-02-26T10:51:07Z
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nmb a2200000Iu 4500
"200522 2020 eng "
2449-8955
dc
Assessment of in-vivo anti-diabetic and anti-diarrheal effects of Flemingia stricta Roxb. leaf
Biozid, Md. Shahrear
Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Alam, Mohammad Nazmul
Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Abeden, Md. Jainul
Department of Pharmacy, International Islamic University Chittagong, Bangladesh
Faruk, Md.
Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Chowdhury, Ahmad Ibtehaz
Department of Pharmacy, International Islamic University Chittagong, Bangladesh; Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
Sajib, Muzahidul Islam
Department of Pharmacy, International Islamic University Chittagong, Bangladesh
Rahman, Md. Masudur
Department of Pharmacy, International Islamic University Chittagong, Bangladesh
Islam, Md. Rafikul
Department of Pharmacy, International Islamic University Chittagong, Bangladesh
The objective of this study was to evaluate the anti-diabetic and anti-diarrheal activity of methanol extract of Flemingia stricta Roxb. (Fabaceae) leaf. In anti-diabetic study, the extract was administered to alloxan-induce diabetic mice at two concentrations (200 mg/kg and 400 mg/kg body weight) for acute (12 hours) and prolong treatments (15 days) and blood glucose levels of diabetic mice were monitored at intervals of hours and days throughout the duration of treatment. Antidiarrheal test was conducted by castor oil induced diarrhea and enteropooling as well as intestinal motility in mice at three different concentration (100 mg/kg, 200 mg/kg and 400 mg/kg body weight). Treatment of alloxan induce diabetic mice with the extract caused a significant reduction in fasting blood glucose level of the diabetic mice both in acute (12 hours) and prolong treatment (15 days) and it was determined that the F. stricta methanol extract at both concentration (200 mg/kg and 400 mg/kg) showed the significant (P<0.05) hypoglycemic effect in comparison to the standard drug metformin. In the case of castor oil induced diarrheal test, enteropooling test and gastrointestinal motility test, the extract of F. stricta at 100 mg/kg, 200 mg/kg and 400 mg/kg has given significant effect (P<0.05) compared to the standard drug loperamide. But 400 mg/kg demonstrated the highest activity amongst the three doses. These results suggested that the methanol extract of F. stricta Roxb. possess promising anti-diabetic effect on alloxan-induced mice and significant antidiarrheal effect on castor oil induced diarrheal mice.
DOI: http://dx.doi.org/10.5281/zenodo.3839749
JBródka Publisher
2020-04-14 14:28:50
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/266
European Journal of Biological Research; Vol 10 No 2 (2020): April-June 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/272
2021-02-26T10:51:06Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200606 2020 eng "
2449-8955
dc
Understanding the phenomena of extraction of essential oils by the microwave accelerated distillation process: case of the Washington Navel variety
Boutemtam, Leila
Laboratory of Research on Bio-active Products and Valorization of Biomasse, Ecole Normale Supérieure, vieux-Kouba 16050, Alger, Algeria
Boukhatem, Mohamed Nadjib
Department of Cell Biology and Physiology, Faculty of Natural and Life Sciences University Saad-Dahleb–Blida 1, Blida, Algeria
Messaoudi, Mohammed
Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, 17200 Djelfa, Algeria
Begaa, Samir
Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, 17200 Djelfa, Algeria
Benarfa, Adel
Laboratory of Fundamental Sciences, University Amar Telidji of Laghouat, P.O. Box. 37G, Road of Ghardaïa, 03000 Laghouat, Algeria
Ferhat, Mohamed Amine
Laboratory of Research on Bio-active Products and Valorization of Biomasse, Ecole Normale Supérieure, vieux-Kouba 16050, Alger, Algeria
In this study, two techniques Hydrodistillation (HD) and Microwave-accelerated distillation (MAD), was used to extract essential oils (EOs), from the peels of citrus fruits Washington Navel (Citrus sinensis L. Osbeck) collected from Tipaza province, north Algeria during April 2018. The extraction yield and time of EOs were (0.28% and 180 min) using HD extraction and (0.27% versus 30 min) using MAD extraction. After using gas chromatography analyses (GC-FID) and (GC-MS), 21 aromatic compounds obtained and identified for both extraction approaches. On the other hand, and in order to better comprehension the extraction phenomena, two models of extraction processes were applied, The first one considers the existence of a single site with a constant speed ruled by the equation of the quasi-stationary state, whereas, the second assumes that there are two distinct sites: The first is part of the fraction easily accessible with a very high desorption rate k1 (fast fraction), the second contains the fraction that is difficult to extract, with a low desorption speed k2 (slow fraction). The results showed that, the application of the two sites model can describe accurately the used extraction methods in this study. The data from HD extraction modeling indicate that this method extraction is fast fraction (f equal 0.79), then MAD method (f equal 0.40).
DOI: http://dx.doi.org/10.5281/zenodo.3881852
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/272
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/281
2021-02-26T10:51:06Z
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driver
nmb a2200000Iu 4500
"200626 2020 eng "
2449-8955
dc
Cladistics analysis of Calycanthaceae on the basis of morphological and anatomical characters
Paudel, Niroj
Department of Applied Plant Science, Kangwon National University, Chuncheon 24341, Republic of Korea
Heo, Kweon
Department of Applied Plant Science, Kangwon National University, Chuncheon 24341, Republic of Korea
Cladistics analysis was carried out to find the correct phylogenetic relationship of the four genera of Calycanthaceae. Morphological and anatomical information from all aspect of the data were considered for the analysis. Siparuna guianensis (Siparunaceae) and Cinnamomum malabatrum (Lauraceae) were considered as out-group. Characters were selected mainly for reasonable argument of the similarity. Character-state transformation and discrimination of the genera were decided based upon the out-group comparison method. PAUP* (ver. 4.0) program was used for the dataset analysis and to make phylogenetic tree. The genera split into two separate genera Idiospermum + Chimonanthus and Sinocalycanthus + Calycanthus. Chimonanthus + Idiospermum are supported strict consensus tree with f-value calculation. Furthermore, Sinocalycanthus and Calycanthus are separated genera. Therefore, Calycanthaceae be redefined wide circumscriptions of the characters. The detailed investigation of the cladistics analysis revealed that the Sinocalycanthus and Calycanthus are the sole genus.
DOI: http://dx.doi.org/10.5281/zenodo.3908965
Keywords: Calycanthaceae, Characters and characters state, Cladistics, Out-group, Phylogeny
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/281
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/282
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driver
nmb a2200000Iu 4500
"200629 2020 eng "
2449-8955
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In-silico study of some natural compounds used as antifungal agents against Candida albicans
Srivastava, Ankita
Department of Biotechnology, SR Institute of Management and Technology, Lucknow - 226201, India
-, Neelabh
Department of Biotechnology, SR Institute of Management and Technology, Lucknow - 226201, India
Fungal diseases are very common these days, so there is a high need to design and develop new antifungal drugs that can counter these diseases. Candida albicans is one of the opportunistic pathogenic yeasts that can cause serious diseases such as oropharyngeal candidiasis, vulvovaginal (genital) candidiasis, and invasive candidiasis (candidemia). This article focuses on the in-silico evaluation of anti-candidal activity of some natural compounds like ajoene, allicin, curcumin, gingerol, nimbin, nimbolide, nimonol and 6-Shogaol. Binding affinity of these compounds have been determined against the most common targets in C. albicans viz. cytochrome p450, lanosterol synthase, serine/threonine protein kinase, squalene monooxygenase, sterol-14-demethylase and thymidylate synthase. PatchDock and FireDock web servers were used to carry out the docking studies. The proposed targets of ajoene, allicin, curcumin, gingerol, nimbin, nimbolide, nimonol and 6-Shogaol are sterol 14-demethylase, cytochrome p450, cytochrome p450, cytochrome p450, cytochrome p450, squalene monooxygenase, lanosterol synthase and squalene monooxygenase respectively based upon the binding energies obtained by the docking studies. This study opens new avenues in the usage of the natural compounds as potential antifungal agents.
DOI: http://dx.doi.org/10.5281/zenodo.3911299
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/282
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/284
2021-02-26T10:51:04Z
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nmb a2200000Iu 4500
"200801 2020 eng "
2449-8955
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High level dietary inclusion of monosodium glutamate lowers daily sperm production and efficiency in cocks
Olarotimi, Olumuyiwa Joseph
Animal Physiology Unit, Department of Animal Science, Faculty of Agriculture, Adekunle Ajasin University, P.M.B 001, Akungba-Akoko, Nigeria; Animal Physiology Laboratory, Department of Animal Production and Health, School of Agriculture and Agricultural Technology, The Federal University of Technology Akure, P.M.B. 704, Akure, Nigeria https://orcid.org/0000-0002-5934-2529
Adu, Olufemi Adesanya
Animal Physiology Laboratory, Department of Animal Production and Health, School of Agriculture and Agricultural Technology, The Federal University of Technology Akure, P.M.B. 704, Akure, Nigeria https://orcid.org/0000-0001-8967-7752
Olarotimi, Abosede Oluwakemi
Chemistry Laboratory, Department of Research and Development, Engineering Materials Development Institute (EMDI), KM 4, P.M.B. 611, Akure, Nigeria https://orcid.org/0000-0001-7140-5716
In a 16-week feeding trial, an investigation was carried out with 240 sexually matured cocks of twenty 24 weeks of age to assess the daily sperm production (DSP) and sperm production efficiency (SPE) of cocks fed dietary monosodium glutamate (MSG) at varied inclusion levels (0.00, 0.25, 0.50, 0.75, 1.00 and 1.25 g/kg diet designated diets A, B, C, D, E and F, respectively). The cocks were weighed (1888.33 ± 44.10 kg) and allotted to the 6 treatment diets. Each treatment was replicated 5 times with 8 cocks/ replicate in a completely randomized design. At the end of the feeding trial, 2 cocks per replicate (i.e. 4 cocks per treatment) were humanely sacrificed and their reproductive tracts dissected. The testes were carefully sampled, weighed and processed for estimation of DSP and SPE using both histological and homogenate methods of analyses. Results showed that the inclusion of MSG at 1.25 g/kg significantly reduced the DSP under the two estimation methods (P<0.05). The SPE was equally significantly lowered at 0.75 g MSG/kg diet and above when determined using the homogenate method. It was also observed that MSG at 1.00 g/kg diet and above lowered the DSP and SPE when determined histometrically. A high positive correlation was established between the DSP and the testicular volume of the cocks. However, the paired testicular sperm reserves were not significantly influenced (P≥0.05). Sperm reserves in both testicles of the cocks fed diets B and C were similar to the control. This study therefore, suggests that MSG has a potential to significantly reduce the reproductive potentials of cocks when administered in excess of 0.75 g/kg diet.
DOI: http://dx.doi.org/10.5281/zenodo.3969026
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/284
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/299
2021-02-26T10:51:05Z
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driver
nmb a2200000Iu 4500
"200716 2020 eng "
2449-8955
dc
Cytomorphological changes in the cerebral and ventral ganglionic neurosecretory cells during copulation in epigeic earthworms
Banik, Dipanwita
Department of Zoology, Tripura University, Tripura, India
Chaudhuri, Priyasankar
Department of Zoology, Earthworm Research Laboratory, Tripura University, Suryamaninagar-799022, Tripura, India
Bhattacharjee, Anurag
Department of Zoology, Ramthakur College, West Tripura, India
In spite of hermaphroditism, most earthworm species reproduce by cross fertilization i.e. by the process of copulation of two mature partners. Mechanism of copulation in few earthworm species (Lumbricus terrestris) is known. However literature on neurosecretory control of copulation in earthworm is lacking. In the present study 30 pairs of conjugating earthworms of Eisenia fetida were collected from laboratory culture beds during 2016 of monsoon season. Cerebral and ventral ganglia of 10 pairs of conjugating earthworms and 20 pairs of post conjugation (2 hrs after separation, 4 hrs after separation) earthworms were fixed in Bouin’s fluid for cytomorphological studies on their cerebral neurosecretory cells. Earthworms (10 numbers) debrained through anterior transection of first five segments (brain present in 3rd segment) by sterilized paragon knife were studied to observe conjugation if any. Cerebral and ventral ganglia of 5 pairs of pre-conjugating earthworms were considered as control. Cerebral and ventral ganglia of earthworms displayed chiefly two types of neurosecretory cells such as A cells and B cells. The A cells are deeply stained AF-positive cells arranged in upper cortical tier beneath the perineurium and generally exhibiting process of axonal transport. The B cells, generally larger in size than A cells, are lightly or moderately stained with or without axonal transport and lie in between cortical A cells and central fibrous neuropile. In the cerebral ganglia the A cells outnumbered the B cells, while in the ventral ganglia the opposite is true. Debrained E. fetida survived but did not conjugate. Secretory dynamics in the cerebral and ventral ganglionic neurosecretory cells A and B were recorded in pre-conjugating, conjugating and post-conjugating earthworms. Absence of conjugation in debrained worms and exhibition of the same in earthworms with brain and its changes in neurosecretory profile clearly indicate involvement of cerebral neurosecretion in the phenomenon of conjugation in earthworm. Our result indicates probable involvement of cerebral neurohormone in the process of conjugation in E. fetida.
DOI: http://dx.doi.org/10.5281/zenodo.3948600
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/299
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/302
2021-02-26T10:51:04Z
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driver
nmb a2200000Iu 4500
"200801 2020 eng "
2449-8955
dc
Evaluation of health risk in relation to geohelminths in dumpsites of Ondo Town, Nigeria
Simon-Oke, Iyabo Adepeju
Parasitology and Public Health Unit, Department of Biology, Federal University of Technology, Akure, Nigeria
The study evaluated the contamination level of geohelminths and the health risk in two major dumpsites at Ondo town of Ondo State. One hundred and eighty soil samples were collected from randomly selected sites through the use of quadrant between May and July, 2018 from two locations. Soil samples were analyzed for the presence of helminth ova and larvae following standard procedures. The patterns of parasite prevalence in relation to soil physiochemical parameters were determined. 168 (93.3%) soil samples were positive after analyses for different parasite stages. Epe soil recorded higher prevalence of 88 (97.8%) and the sub-soil had higher number of parasites 94 (52.2%) than the top soil 74 (41.1%). Parasite types found included hookworm (Necator americanus) (36.7%), Strongyloides stercoralis (15.6%), Ascaris lumbricoides (28.9%) and Trichuris trichuria (18.9%). The mean soil temperature and pH were 27.9 ± 0.7°C and 6.0 ± 0.5% respectively. The presence of viable soil transmitted helminths (STHs) eggs/larvae in soil suggest possible active transmission and high rate of exposure to infective agents among the inhabitants. There is a need for health education on risk associated with STH infection in the environment and public investments on sanitation that is essential to, protect individuals, control geohelminths and other sanitary related infectious diseases.
DOI: http://dx.doi.org/10.5281/zenodo.3969551
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/302
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/304
2021-02-26T10:51:02Z
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driver
nmb a2200000Iu 4500
"200831 2020 eng "
2449-8955
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Validation of Ganoderma lucidum against hypercholesterolemia and Alzheimer’s disease
Rahman, Mohammad Azizur
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh; Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Hossain, Md Shahdat
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh
Abdullah, Noorlidah
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
Aminudin, Norhaniza
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia; University of Malaya Centre for Proteomics Research, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
Ganoderma lucidum has been hailed as medicinal mushroom. Its effect on memory and learning related behavioral performance along with related protein markers has been evaluated using Alzheimer’s disease (AD) and hypercholesterolemic model rats in the present study. AD model rats were prepared infusing amyloid beta peptide into the right ventricles of the rats. Hypercholesterolemia was evoked feeding 1% cholesterol and 1% cholic acid with basal diet of the rats for 8 weeks. Hot water extract of G. lucidum was ingested orally (200 mg/kg bw) to the HC and AD model rats. Memory and learning related behavioral tests were performed using Barnes maze while protein markers (BDNF, SNAP2, PSD-95, VAchT) were detected using ELISA. Observed findings suggest hypocholesterolemic, lipid profile improving and enhanced cognitive performance of the G. lucidum fed rats. Memory and learning related protein markers also substantiate this fruition. Thus, therapeutic potentiality of Ganoderma lucidum in AD amelioration seems promising.
DOI: http://dx.doi.org/10.5281/zenodo.4009588
JBródka Publisher
2020-08-19 00:10:19
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/304
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
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oai:ojs2.journals.tmkarpinski.com:article/305
2021-02-26T10:51:03Z
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nmb a2200000Iu 4500
"200816 2020 eng "
2449-8955
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Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber
Bertan, Alessandra Suzin
School of Chemical Engineering, University of Campinas, 13083-852, Campinas, Sao Paulo State, Brazil
Cremasco, Marco Aurélio
School of Chemical Engineering, University of Campinas, 13083-852, Campinas, Sao Paulo State, Brazil
The present work presents an alternative technique to obtain the concentration of pseudomycelles as number of pseudomycelles/sample volume, obtained by Neubauer chamber, as function of absorbance from spectroscopy. Samples of broth from fermentation via bacteria Streptomyces tsukubaensis were analyzed, making it possible to obtain a linear relationship between the number of pseudomycelles/ sample volume and absorbance, with determination coefficient of 0.995. In addition, a morphological analysis of the unfermented pseudomycelles was performed using scanning electron microscopy (SEM) at the magnifications of 20, 2000, 5000, 10000 and 20000 x.
DOI: http://dx.doi.org/10.5281/zenodo.3987017
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/305
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/306
2021-02-26T10:51:05Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200711 2020 eng "
2449-8955
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Jamun seed and orange peel extracts protects effects of microcystin LR on serum calcium and phosphate of rats
Srivastava, Babita Deep
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Srivastava, Manish
Department of Chemistry, Digvijai Nath P.G. College, Gorakhpur, India
Srivastav, Sunil Kumar
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Urata, Makoto
Institute of Noto Satoumi Education Research, Noto‐cho, Ishikawa 927‐0553, Japan; Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Noto‐cho, Ishikawa 927‐0553, Japan
Suzuki, Nobuo
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Noto‐cho, Ishikawa 927‐0553, Japan
Srivastav, Ajai Kumar
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Wistar rats were treated as Group A: Control; Group B: microcystin LR (MCLR); Group C: microcystin LR and jamun seed extract (MCLR+JSE); Group D: microcystin LR and orange peel extract (MCLR+OPE); Group E: orange peel extract (OPE); Group F: jamun seed extract (JSE). MCLR dose was 10 µg/kg body wt/day whereas OPE and JSE dose was 200 mg/kg body wt/day. Serum calcium and phosphate were analyzed on 15 and 30 day. Serum calcium of rat exposed for 15 day to MCLR; MCLR+JSE and MCLR+OPE decreased. Although there is little increase in levels of group C and group D but treatment with OPE and JSE is not able to completely restore decreased calcium levels caused by MCLR. After 30 day calcium decreased after MCLR; MCLR+JSE and MCLR+OPE treatment. Levels in group E and F remain unaltered. Levels in group C and D exhibit elevation as compared to group B which indicates that treatment with OPE and JSE recovered calcium. Serum phosphate decreased after 15 day in MCLR; MCLR+JSE and MCLR+OPE treated rats. Phosphate levels of group C (compared with group F) and group D (compared with group E) show decrease. After 30 day exposure to MCLR; MCLR+JSE and MCLR+OPE phosphate levels decreased. Levels of group C and D when compared with group B are increased. Phosphate levels of group C (compared with group F) and D (compared with group E) are decreased. This indicates that OPE and JSE treatment provoked some recovery of phosphate levels.
DOI: http://dx.doi.org/10.5281/zenodo.3940650
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/306
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/307
2021-02-26T10:51:03Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200812 2020 eng "
2449-8955
dc
Effect of growth media on the early performance of Prosopis africana (Guill. and Perr.) Taub. seedlings
Ancha, Paul U.
Department of Social and Environmental Forestry, Federal University of Agriculture, Makurdi, Nigeria
Chukwu, Onyekachi
Department of Forestry and Wildlife, Nnamdi Azikiwe University, Awka, Nigeria https://orcid.org/0000-0002-6376-6084
Ezeano, Caleb I.
Department of Agricultural Economics and Extension, Nnamdi Azikiwe University, Awka, Nigeria
Udekwe, Maryprecious A.
Department of Forestry and Wildlife, Nnamdi Azikiwe University, Awka, Nigeria
Iheme, Francisca C.
Department of Forestry and Wildlife, Nnamdi Azikiwe University, Awka, Nigeria
This study examined the performance of Prosopis africana seedlings raised under different growth media. The experiment was laid in a completely randomized design with three treatments (topsoil, mixture of topsoil and poultry droppings, and mixture of topsoil and cow dung). Each treatment was replicated 4 times and each replicate had 10 potted seedlings making a total of 120 seedlings. The seedling growth variables; heights (cm) collar diameters (cm), number of leaves data were collected every 3 days for 12 weeks. The data collected were subjected to analysis of variance and significant means were separated using Duncan multiple range test at 0.05 significant level. The result showed significant difference (p=0.005) in the height of seedlings. No significant difference existed in the seedlings collar diameter (p=0.306) and number of leaves (p=0.957). The follow-up result indicated that there was no significant difference (p>0.05) in the height of seedlings raised in mixture of topsoil and poultry droppings (Mean ± standard error =105.87±5.40a) and topsoil (105.18±4.27a). However, a significant difference (p>0.05) existed between both treatments and mixture of topsoil and cow dung (86.36±4.90b). Seedlings raised in the mixture of topsoil and poultry droppings had the best performance. The study therefore, recommended raising Prosopis africana seedling with topsoil for reduced cost of production.
DOI: http://dx.doi.org/10.5281/zenodo.3980933
JBródka Publisher
2020-06-06 15:41:27
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/307
European Journal of Biological Research; Vol 10 No 3 (2020): July-September 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/313
2021-02-26T10:51:01Z
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driver
nmb a2200000Iu 4500
"200911 2020 eng "
2449-8955
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Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B
Jibrin, Amina Musa
Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
Oyewole, Oluwafemi Adebayo
Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
Yakubu, Japhet Gaius
Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
Hussaini, Aisha
Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
Egwim, Evans Chidi
Department of Biochemistry, Federal University of Technology Minna, Niger State, Nigeria
Biosorption is the ability of biological materials to accumulate heavy metals from wastewater through mediated or physico-chemical pathways of uptake. Urease producing bacteria have been hypothesized to have inherent bioremediation abilities. The aim of this research was to determine the potential of Lysinibacillus fusiformis 5B to biosorp Pb, Cr, Cd and Ni. The stock solution of Pb, Cr, Cd and Ni was prepared by dissolving 0.0157 g of Pb(C2H3O2)2, 0.057 g of K2Cr2O7, 0.018 g of CdSO4 and 0.026 g of NiSO4 in 100 mL of dH2O respectively. Lysinibacillus fusiformis 5B was screened for the potential to utilise 5 ppm of the heavy metals using agar dilution method. Broth of L. fusiformis 5B was inoculated to 10, 15, 20 and 50 ppm of the heavy metals. The rate of biosorption was determined by atomic absorption spectroscopy (AAS) after 0, 7, 14, 21, 28 and 35 days. The biosorption % was determined by Beer Lambart’s equation. Lysinibacillus fusiformis 5B was able to tolerate 5 ppm concentration of all the heavy metals by showing visible growth on surfaces of nutrient agar Petri plates. Generally, there was an increase in biosorption rate as the days progress. After 35 days of incubation, the highest biosorption rate of 99.96%, 99.97%, and 99.94% were recorded for Pb, Cr, and Cd respectively at 10 ppm and 99.33% of Ni at 15 ppm. The results of this study showed that L. fusiformis 5B possess the capacity to biosorp Pb, Cr, Cd and Ni and can be developed as biosorption agent for these heavy metals.
DOI: http://dx.doi.org/10.5281/zenodo.4023161
JBródka Publisher
2020-08-19 00:10:19
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/313
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/318
2021-02-26T10:51:02Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200827 2020 eng "
2449-8955
dc
Effects of chlorpyrifos on ultimobranchial and parathyroid glands of Indian skipper frog, Euphlyctis cyanophlyctis
Srivastav, Ajai Kumar
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Srivastava, Shilpi
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Kumar, Abhishek
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Srivastav, Sunil Kumar
Department of Zoology, DDU Gorakhpur University, Gorakhpur 273009, India
Suzuki, Nobuo
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Noto‐cho, Ishikawa 927‐0553, Japan
This study investigated effects of chlorpyrifos on ultimobranchial (UBG) and parathyroid glands (PTG) of frog, Euphlyctis cyanophlyctis. Frogs were treated with chlorpyrifos for short and long term and sacrificed after 24, 48, 72 or 96 h in short term and after 5.10, 15 and 30 days in long term. Chlorpyrifos exposure provokes decrease in serum calcium levels after 48 h which persists till 96 h. There is slight decrease in the nuclear volume of UBG cells and cytoplasm depict weak staining response after 72 h. After 96 h these changes are more pronounced. PTG of Euphlyctis cyanophlyctis exposed to chlorpyrifos exhibit no change till 96 h. Serum calcium decreases on day 10 after chlorpyrifos exposure which continue to fall progressively till 30 days. After 15 days chloryrifos exposure, nuclear volume of UBG exhibit decrease and follicular epithelium displays decrease in height. Follicular epithelium after 30 days chlorpyrifos exposure reduces to the extent that it becomes single layered. Few degenerating cells have been discerned. At this interval nuclear volume of ultimobranchial cells exhibits a further decrease. PTG of chlorpyrifos treated frog depicts increased nuclear volume of PTG at 10 and 15 days. The nuclei of PTG are hyperchromatic and the gland becomes compact at 15 days. After 30 days following chlorpyrifos treatment nuclear volume exhibits further increase. Also degenerating cells make their appearance. Calcium regulating glands UBG and PTG of frogs were adversely affected by exposure to chlorpyrifos which may disturb the physiological functions of the organism.
DOI: http://dx.doi.org/10.5281/zenodo.4004156
JBródka Publisher
2020-08-19 00:10:19
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/318
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/319
2021-02-26T10:51:01Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200926 2020 eng "
2449-8955
dc
Estimation of post-harvest losses of Manfalouty pomegranate fruits
El-Orabi, Samya G. E.
Fruit Handling Department, Horticultural Research Institute, Agricultural Research Centre, (Affiliation ID: 60019332), 9 Gamaa Street, 12619, Giza, Egypt
Hassan, Amal Moustafa
Fruit Handling Department, Horticultural Research Institute, Agricultural Research Centre, (Affiliation ID: 60019332), 9 Gamaa Street, 12619, Giza, Egypt
Mansour, Ahmed Hassan
Fruit Handling Department, Horticultural Research Institute, Agricultural Research Centre, (Affiliation ID: 60019332), 9 Gamaa Street, 12619, Giza, Egypt
Weight loss considered one of the main causes of quality loss in pomegranate fruits during chain marketing. Therefore, this study was conducted on Manfalouty pomegranate (Punica granatum L.) in a private orchard in El Badary, Assiut Governorate, Egypt in 2017 and 2018 to define the various causes of losses during chain handing and estimate it. The fruits harvested at three periods early (September) mid (October) and late season (November). The total losses at harvest were 5.94%, 9.30% and 23.50% for early, mid and late season, respectively. The main cause of losses is due to cracked and infected pests. The total loss of fruits during chain marketing was highest in retail market in comparison with wholesale during early, mid and late season. The main causes of losses due to weight loss and shrinkage fruits. According to data dealing with storage pomegranate fruits at 5±1°C and relative humidity 85-90%, the highest fruit losses found in the third month and this losses due to fruit weight loss and internal chilling injury (brown discoloration) so the storage life of fruit should be two months.
DOI: http://dx.doi.org/10.5281/zenodo.4051220
JBródka Publisher
2020-08-19 00:10:19
application/pdf
http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/319
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/333
2021-02-26T10:51:00Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201029 2020 eng "
2449-8955
dc
Molecular docking studies on binding specificity of 3,6- and 2,7-carbazoles with DNA duplexes
Pandey, Anwesh
Department of Physics, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, U.P., India
Misra, Manas
Department of Physics, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, U.P., India
Yadav, Anil Kumar
Department of Physics, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, U.P., India
Molecular docking is a widely used computational technique used to find the probabilistic binding sites of drugs in the vicinity of macromolecules. The drugs produce their working effect only when they bind and interact with the target macromolecule. The potential drugs can only be identified by their relative binding affinities and corresponding binding modes. Availability of huge numbers of such drugs has made the estimation of their relative potency, a difficult task. In the present work, carbazoles (3,6 and 2,7) and their analogs were studied for their DNA binding abilities using molecular docking calculations. Since the docked ligands had planar structures, it allowed them to adopt crescent shape and thus minor groove binding with DNA was preferred by all. However, it was found that a single molecule (Mol-6) (2,7-carbazole) showed promising results with all the selected DNA sequences also its results were exactly verified with those in the reported literature and therefore it can be said that its in-vivo studies could possibly produce some exciting results. This study also revealed that DNA binding energies of 3,6- and 2,7-carbazoles followed the same trend as their thermal melting values.
DOI: http://dx.doi.org/10.5281/zenodo.4153709
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/333
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/336
2021-02-26T10:51:00Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201021 2020 eng "
2449-8955
dc
Beneficial effects of ascorbic acid on ivermectin repeated high-dose therapy in rabbits: biochemical and histopathological investigations
Chahrazed, Makhlouf
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria; Natural Resources Laboratory, University Mouloud Mammeri, BP 15017, Tizi-Ouzou, Algeria
Hassina, Khaldoun Oularbi
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria; Natural Resources Laboratory, University Mouloud Mammeri, BP 15017, Tizi-Ouzou, Algeria
Soumya, Bokreta
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria; Natural Resources Laboratory, University Mouloud Mammeri, BP 15017, Tizi-Ouzou, Algeria
Dalila, Tarzali
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria
Asma, Boukrid
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria
Meriem, Boulahia
Department of Biology, Faculty of Nature and Life Sciences, University Blida 1, BP 270, Soumaa, Blida, Algeria
Nacira, Daoudi Zerrouki
Natural Resources Laboratory, University Mouloud Mammeri, BP 15017, Tizi-Ouzou, Algeria
Ivermectin (IVM) is a lipophilic anthelmintic drug widely used for the control of internal and external parasites in both human and veterinary medicine. Conversely, overdoses of IVM are associated with resistance and efficacy problems. The present study aimed to evaluate the effects of repeated administration of a high dose of IVM alone or with combination of ascorbic acid (AA) in male young rabbits (Oryctolagus cuniculus) via biochemical and histological investigations. Twenty rabbits were divided into four groups (n=5) and treated for three consecutive weeks: Control group; IVM group (2 mg/kg of body weight subcutaneously, 3 times a week); IVM + AAg (20 mg/mL) group and IVM + AAf (200 mg/kg of diet) group. IVM induced a disruption of hepatic biochemical parameters and lipid profile with a statistically significant (p < 0.05) increase in glucose, ALT, AST, GGT, HDL-C and a significant decrease of TC, TG, LDL-C, VLDL-C in IVM group compared to control group. Co-administration of AA moderately improved those biochemical parameters. Histopathological changes following IVM treatment in liver comprised loss of normal hepatocytes structure, central vein dilation and portal vein congestion. The lung showed abnormal structure of intrapulmonary bronchus, dilated bronchioles and alveoli and congested pulmonary artery. Nevertheless, the AA treatment groups revealed significant improvement when co-administered orally with IVM. This study suggested that AA has a beneficial ameliorative role against toxic effects induced by repeated high-dose of IVM.
DOI: http://dx.doi.org/10.5281/zenodo.4116306
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/336
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/338
2021-02-26T10:50:59Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201129 2020 eng "
2449-8955
dc
Direct detection of Mycobacterium tuberculosis with nitrate reductase assay and microscopic observation drug susceptibility
Falodun, Olutayo Israel
Department of Microbiology, University of Ibadan, Nigeria
Cadmus, Idowu Simeon
Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Nigeria
Fagade, Obasola Ezekiel
Department of Microbiology, University of Ibadan, Nigeria
The global increase in tuberculosis drug resistant which is a threat to its control, require low cost method of diagnosis and detection. Available conventional and molecular methods consume time, and are expensive for countries with high disease burden. Nitrate Reductase Assay (NRA) and Microscopic Observation Drug Susceptibility (MODS) performance to directly detect tuberculosis resistance to four drugs was evaluated. The NRA (liquid and solid) and MODS performance of smear-positive sputum samples were evaluated; Sensitivities and specificities were compared with Proportion Method (PM). Sensitivity and specificity of liquid NRA (LNRA) were 90% and 98% (rifampicin), 81.8% and 100% (isoniazid), 88.9% and 98.1% (streptomycin), and 57.1% and 94.4% (ethambuthol). Also, the sensitivity and specificity for solid NRA (SNRA) were 69.2% and 98.3% (rifampicin); 100% and 100% (isoniazid); 88.9% and 95.2% (streptomycin); 70% and 80.6% (ethambuthol). Moreover, For MODS, rifampicin and isoniazid sensitivity and specificity was 100%, it was 100% and 98.1% for streptomycin, and 71.4% and 98.2% for ethambuthol. At day 14, the results available for LNRA, SNRA and MODS were 93%, 68.5% and 100% respectively. The agreement between LNRA and PM was 97% (RIF, INH and SM) and 90% (EMB). For SNRA, it was 93% (RIF), 100% (INH), 94% (SM) and 89% (EMB). While for MODS, it was 100% (RIF and INH), 98% (SM) and 95% (EMB). Direct NRA and MODS are sensitive, reliable and fast for antituberculosis drug susceptibility; they have potential to effectively and reliably detect drug resistant tuberculosis in the low resource countries.
DOI: http://dx.doi.org/10.5281/zenodo.4295881
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/338
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/341
2021-02-26T10:51:00Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201002 2020 eng "
2449-8955
dc
In silico molecular docking of selected polyphenols against interleukin-17A target in gouty arthritis
Umar, Haruna Isiyaku
Department of Biochemistry, Federal University of Technology, P. M. B. 704, Akure, Ondo State, Nigeria
Ajayi, Adeola
Department of Biochemistry, Federal University of Technology, P. M. B. 704, Akure, Ondo State, Nigeria
Josiah, Sunday Solomon
Department of Biochemistry, Federal University of Technology, P. M. B. 704, Akure, Ondo State, Nigeria
Saliu, Tolulope
Department of Biochemistry, Federal University of Technology, P. M. B. 704, Akure, Ondo State, Nigeria
Danjuma, Jamilu Bala
Department of Biochemistry and Molecular Biology, Federal University, Birnin Kebbi, Kebbi State, Nigeria
Chukwuemeka, Prosper Obed
Department of Biotechnology, Federal University of Technology, Akure, Nigeria
The binding of Interleukin-17A (IL-17A) to its receptor causes the release of chemokine which have an implication in the pathogenesis of gouty arthritis. Though, some synthetic drugs have been proved worthy as IL-17A inhibitors in the management of gout but they have been associated with a number of side effects. Polyphenols have been documented for numerous therapeutic applications. In spite of this, there are scarce data on the mechanism of action and protective potentials of polyphenolic against gouty arthritis. This present in silico study aimed to assess the inhibitory potentials and ADMET properties of selected polyphenols against IL-17A using molecular docking tools. The crystal structure of IL-17A was retrieved from the protein database, while the structures of polyphenolic compounds were retrieved from Pubchem. Drug-likeness of the polyphenols was assessed using DruLiTo. A total of 22 out of 26 polyphenols investigated passed the Lipinski drug likeness rule of five which were then docked with the active site of IL-17A using docking software, and the docked complexes were analyzed using LigPlot and protein-ligand profiler web server. The results showed that all the investigated polyphenols have appreciable higher binding affinity when compared to the standard drug (allopurinol) with pelargondin and catechin having the highest binding affinity (-7.5 kcal/mol). Furthermore, ADMET screening were carried out on the five compounds with the best hits. Conclusively, this in silico study suggests that these investigated polyphenols could serve as better replacements for synthetic drugs such as allopurinol in the management of gouty arthritis.
DOI: http://dx.doi.org/10.5281/zenodo.4064236
JBródka Publisher
2020-08-19 00:10:19
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/341
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/343
2021-02-26T10:51:01Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"200929 2020 eng "
2449-8955
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Antioxidant activity of extracts formulated from Citrus aurantium and Artemisia herba alba
Boukhennoufa, Asma
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Faculty SNV, University of Mascara, Algeria
Benmaghnia, Souhila
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Faculty SNV, University of Mascara, Algeria
Meddah, Boumediene
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Faculty SNV, University of Mascara, Algeria
Tir Touil Meddah, Aicha
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, Faculty SNV, University of Mascara, Algeria
Plants still present a large source of novel active biological compounds with different activities. The antioxidant activity of three extracts was evaluated by DPPH and ferric reducing antioxidant power methods. The formulated extracts were analyzed by thin layer chromatography (TLC) and then confirmed by High performance liquid chromatography coupled with DAD detector (HPLC-DAD). The results showed the richness of these extracts in phenolic compounds. Three major compounds, resveratrol (17.98%), kaempferol-glucoside (7.23%) and vanillic acid (10.64%) were detected in methanolic extract of Citrus aurantium peel, aqueous extract of Citrus aurantium L. leaves and ethanolic extract of Artemisia herba alba Asso respectively by HPLC-DAD. However, the ethanolic extract of A. herba alba achieved 50% of the anti-radical activity at a concentration equal to 0.8 mg/ml. A higher antioxidant activity measured by ferric reducing antioxidant power was marked in the same extract with an absorbance equal to 0.824. The ethanolic extract of the aerial part of A. herba alba, the methanolic extract of C. aurantium peel and the aqueous extract of C. aurantium leaves were considered as powerful scavengers of free radicals and can be incorporated into the pharmaceuticals preparations to treat many diseases.
DOI: http://dx.doi.org/10.5281/zenodo.4058836
JBródka Publisher
2020-08-19 00:10:19
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/343
European Journal of Biological Research; Vol 10 No 4 (2020): October-December 2020
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/349
2021-02-26T10:50:55Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"210118 2021 eng "
2449-8955
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Investigation of the effects of 50 Hz electromagnetic field on the lifespan of the red blood cells in vitro
Elmas, Sinem
Mugla Sitki Kocman University, Faculty of Sciences, Department of Biology, Mugla, Turkey
Elmas, Onur
Mugla Sitki Kocman University, Faculty of Medicine, Department of Physiology, Mugla, Turkey
Zeybek, Ahmet
Mugla Sitki Kocman University, Faculty of Sciences, Department of Biology, Mugla, Turkey
In recent years, studies have indicated that electromagnetic fields (EMFs) may have harmful effects on human health. The effects on human health of the 50 Hz extremely low frequency EMF (ELF-EMF), which is often used in daily life, are still controversial. In our study, we investigated the in vitro effects of 50 Hz ELF-EMF on the lifespan of erythrocytes, which have no nucleus and organelles, and are therefore relatively more sensitive compared to other cells in the body to any harmful effect that may come from outside. Whole blood obtained from healthy volunteers was exposed to 50 Hz, 0.3 mT ELF-EMF over 35 days. After this time, erythrocytes (red blood cell, RBC) counts in blood, hematocrit (HCT) value, main corpuscular volume (MCV), and erythrocyte osmotic fragility (EOF), an indicator of aging, were examined. At the end of 35 days, RBC and HCT were decreased while MCV and EOF were increased in the blood samples of both the EMF-exposed group and the non-exposed group. However, while there were no statistically significant changes in terms of RBC counts, and HCT between the two groups, it was observed that MCV and EOF increased significantly less in the EMF-exposed group compared to the non-exposed group. These results suggest that 50 Hz ELF-EMF exposure does not affect the lifespan of erythrocytes in vitro, but it may extend erythrocytes’ lifecycles due to a reduction in osmotic fragility of the erythrocytes in in vivo conditions.
DOI: http://dx.doi.org/10.5281/zenodo.4447339
JBródka Publisher
2021-01-05 21:57:52
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/349
European Journal of Biological Research; Vol 11 No 2 (2021): April-June 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/350
2021-02-26T10:50:59Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201104 2020 eng "
2449-8955
dc
Exogenous potassium nitrate alleviates salt-induced oxidative stress in maize
Doğru, Ali
Sakarya University Faculty of Arts and Science Department of Biology, Esentepe, 54187 Sakarya, Turkey
Demirtaş, Ecenur
Sakarya University Faculty of Arts and Science Department of Biology, Esentepe, 54187 Sakarya, Turkey
The effects of the exogenous potassium nitrate application on major antioxidant enzymes, photosynthetic pigment content, malondialdehyde, hydrogen peroxide and free proline were investigated in salt-stressed (75 mM NaCl) maize genotype (ADA 9510). Plants were grown in growth chamber for ten days. After five days of applications (control, 0 mM NaCl), S75 (75 mM NaCl), potassium nitrate (3 mM KNO3) and S75 + potassium nitrate (75 mM NaCl + 3 mM KNO3), plants were harvested. The results showed that salt stress significantly decreased chlorophyll a, chlorophyll b and total chlorophyll contents and increased the activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase. Malondialdehyde, hydrogen peroxide and free proline contents were increased by salt stress. These results showed that salinity led to the oxidative stress and destruction of photosynthetic pigments in maize leaves. The exogenous potassium nitrate application, on the other hand, caused to the increased chlorophyll a, chlorophyll b, total chlorophyll and total carotenoid, elevated level of ascorbate peroxidase and glutathione reductase, and decreased malondialdehyde, hydrogen peroxide and free proline content. This kind of changes may indicate that the exogenous potassium nitrate application activates the antioxidant defence system and counteract the oxidative stress. Thus, it may be concluded that the exogenous potassium nitrate application improves salt tolerance and encourage the growth of maize plants under salt stress at early seedling stage.
DOI: http://dx.doi.org/10.5281/zenodo.4245196
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/350
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/351
2021-02-26T10:50:59Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201203 2020 eng "
2449-8955
dc
Multiple antibiotic resistant index and detection of qnrS and qnrB genes in bacterial consortium of urine samples from clinical settings
Bayode, Michael Tosin
Department of Microbiology, Federal University of Technology, P.M.B. 704, Akure, Nigeria
Olalemi, Adewale Oluwasogo
Department of Microbiology, Federal University of Technology, P.M.B. 704, Akure, Nigeria
Oladejo, Babayemi Olawale
Department of Microbiology, Federal University of Technology, P.M.B. 704, Akure, Nigeria
The multiple antibiotic resistant (MAR) index and detection of resistant genes in the bacterial consortium of urine samples collected from University of Medical Sciences Teaching Hospital, Akure (UNIMEDTH) was evaluated with all microbiological and biotechnological techniques employed utilizing specified standards in this study. Escherichia coli had the highest bacterial count (311.50 ± 0.707 CFU/ml) while Staphylococcus saprophyticus had the least (13.00 ± 2.828 CFU/ml). Enterococcus faecalis, and Pseudomonas aeruginosa isolate showed marked resistance against four classes of antibiotics tested. The MAR index of bacterial isolates ranged from 0.5 to 1.0. Fluoroquinolone-resistant P. aeruginosa identified to be P. aeruginosa via 16S rDNA analysis sequence analysis of 417 base pairs with strain mcbay1 deposited in GenBank with accession number MT423976 was positive for qnrS resistant gene. E. faecalis identified by 16S rRNA sequence analysis of 264 bp of the strain mcbay 2 deposited in GenBank with accession number MT423977 was also positive for qnrB resistant gene. The presence of resistant genes in ciprofloxacin-resistant P. aeruginosa and quinolone-resistant E. faecalis in urine samples further emphasized the need for the regulation of over-the-counter prescription and antibiotic susceptibility survey of anti-pseudomonal and anti-enterococcal quinolones in hospital settings.
http://dx.doi.org/10.5281/zenodo.4304311
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/351
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
oai:ojs2.journals.tmkarpinski.com:article/354
2021-02-26T10:50:58Z
ejbr:RESEARCH
driver
nmb a2200000Iu 4500
"201207 2020 eng "
2449-8955
dc
The concentration of glyphosate in the tap water in Greater Poland Region
Kaszkowiak, Krzysztof
Department of Biology and Environmental Studies, University of Medical Sciences, ul. Rokietnicka 8, 60-806 Poznań, Poland
Kubacki, Tomasz
Department of Biology and Environmental Studies, University of Medical Sciences, ul. Rokietnicka 8, 60-806 Poznań, Poland
Olejniczak, Jacek
Soil and Water Survey Laboratory of Provincial Sanitary-Epidemiological Station, ul. Noskowskiego 21, 61-705 Poznań, Poland
Bondarenko, Igor
Puromedica, ul. Batorowska 30, 62-070 Dąbrowa, Poland
The harmfull effects of glyphosate (N-(phosphonomethyl) glycine) on animal and human health was stated by many researchers. The studies on such effects concerned mainly the people exposed to herbicides. In the environment, glyphosate remains relatively stable, with half-life ranged between a few days to several months or even a year in field studies, depending on soil composition. As this herbicide the widely used all over the world, the monitoring its concentration in everyday food becomes necessary. The aim of the study was to estimate the glyphosate levels in tap water samples collected from different Water Treatment Plants in Greater Poland region. The concentration of glyphosate was measured in 66 randomly collected drinking water samples from separate Water Treatment Plants. Measurements were done using two analytical techniques: enzyme-linked immunosorbent assay and high-performance liquid chromatography technique. Levels of glyphosate in the tested samples were low (0.15±0.07 µg/L). Both assays have been found well suited to the analysis of glyphosate concentrations in the drinking water. The concentration of glyphosate in the tap water is very low, and could be discarded in estimation of daily intake of this herbicide in Great Poland region. So, it is unlikely that drinking water from Water Treatment Plants can be important source of glyphosate contamination in urbanized populations compared to vegetables, fruit and other possible sources.
DOI: http://dx.doi.org/10.5281/zenodo.4310255
JBródka Publisher
2021-01-01 00:00:00
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http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/354
European Journal of Biological Research; Vol 11 No 1 (2021): January-March 2021
eng
##submission.copyrightStatement##
9f09675f499f9ac07705ebd356370cb4