Degradation of surfactant and metal-removal by bacteria from a Nigerian laundry environment

  • Abimbola Olumide Adekanmbi Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
  • Wasiu Oyekunle Oyeladun Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
  • Adedolapo Victoria Olaposi Environmental Microbiology and Biotechnology Laboratory, Department of Microbiology, University of Ibadan, Nigeria
Keywords: Metal-removal, Surfactant degradation, Sodium dodecyl sulphate, Laundry environment, Metal-tolerant bacteria, Dual tolerance, Nigeria


This study aimed at degrading sodium dodecyl sulphate (SDS), a surfactant in the presence of metals using metal-tolerant bacteria from a laundry site. Metal composition of wastewater and sediments from a laundry environment was determined using atomic absorption spectrometry (AAS). Paenibacillus amylolyticus BAL1 (PAB) and Bacillus lentus BAL2 (BLB), earlier reported to tolerate 1000 ppm SDS were screened for metal tolerance. The bacteria were employed in the simultaneous degradation of SDS and metal removal in a batch culture set-up containing SDS and metals for 14 days on a rotary shaker at 250 rpm. Residual SDS and metal concentrations were determined using high performance liquid chromatography (HPLC) and AAS. Copper (Cu), zinc (Zn), and cadmium (Cd) were detected in both laundry wastewater and sediment while chromium (Cr) and nickel (Ni) were only detected in the sediments. The MICs of metals on PAB were: Cu and Zn (500 µg/ml), and Cd (100 µg/ml), while for BLB: Cu (500 µg/ml), Zn (400 µg/ml), and Cd (100 µg/ml). PAB degraded 49.90% of SDS and simultaneously removed 8.3% of Cu, 5.1% of Cd, and 6.6% of Zn, while BLB degraded 54.9% of SDS and simultaneously removed 3.1% of Cu, 39% of Cd, and 3.1% of Zn. A combination of the two bacteria led to 44.3% degradation of SDS, and removal of 11% of Cu, 7.7% of Cd, and 9.8% of Zn. Bacteria from this study possessed both SDS-degradation and metal-removing abilities, and could be useful in the bioremediation of wastewater co-contaminated by surfactants and metals due to their dual tolerance to both compounds.



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1. Ying GG. Fate, behaviour and effects of surfactants and their degradation products in the environment. Environ Int. 2006; 32: 417-431.

2. Ivankovic T, Hrenovic J. Surfactants in the environment. Arch Ind Hyg Toxicol. 2010; 61(1): 95-110.

3. Rosen MJ. Surfactants and interfacial phenomena. J Polymer Sci. 1989; 27(12): 431.

4. Mozia S, Tomaszewska M, Morawski A. Decomposition of non-ionic surfactant in a labyrinth flow photoreactor with immobilized TiO2 bed. Appl Cat Environ. 2005; 59: 155-160.

5. Adac A, Bandyopadhyay C, Pal A. Removal of anionic surfactant from wastewater by alumina-a case study. Physic Eng. 2005; 254: 165-171.

6. Liwarska-Bizukojc E. Effect of selected anionic surfactants on activated sludge flocs. Enzymatic Microbiol. & Technol. 2006; 39: 660-668.

7. Braga JK, Varesche MBA. Commercial laundry characterization. Am J Anal Chem. 2014; 5: 8-16.

8. Comber SDW, Gunn AM. Heavy metals entering sewage-treatment works from domestic sources. Water Environ J. 1996; 10: 2.

9. Angle JS, Chaney RL. Cadmium resistance screening in nitrilotriacetate-buffered minimal media. Appl Environ Microbiol. 1989; 55(8): 2101-2104.

10. Traina SJ, Laperche V. Contaminant bioavailability in soils, sediments and aquatic environments. Proc Natl Acad Sci USA. 1999; 96: 3365-3371.

11. Roane TM, Josephson KL, Pepper IL. Dual-bioaugmentation strategy to enhance remediation of co-contaminated soil. Appl Environ Microbiol. 2001; 67(10): 3208-3215.

12. Knotek-Smith HM, Deobald LA, Ederer M, Crawford DL. Cadmium stress studies: media development. Enrichment, consortia analysis, and environmental relevance. Biometals. 2003; 16(2): 251-261.

13. Adekanmbi AO, Usinola IM. Biodegradation of sodium dodecyl sulphate by two bacteria isolated from wastewater generated by a detergent manufacturing plant in Ibadan. Jordan J Biol Sci. 2017; 10(4): 251-255.

14. Narasimhulu K, Rao PS, Vinod AV. Isolation and identification of bacterial strains and study of their resistance to heavy metals and antibiotics. J Microbial Biochem Technol. 2012; 2: 74-76.

15. Aleem J, Isar TI, Malik A. Impact of long term application of industrial wastewater on the emergence of resistance traits in Azotobacter chroococcum isolated from rhizophere soil. Biores Technol. 2003; 86: 7-13.

16. Singh S, Inamder SP, Finger N, Mitchell MJ, Levia DF, Scott D, Bais H. Quality of dissolved organic matter (DOM) in watershed compartments for a forested Mid-Atlantic watershed. AGU fall meeting, San Francisco, CA, B13D-0508, 2010.

17. Rusconi F, Valton E, Nguyen R, Dufourc E. Quantification of sodium dodecyl sulfate in microliter-volume biochemical samples by visible light spectroscopy. Anal Biochem. 2001; 295: 31-37.

18. Im SH, Jeongb YH, Ryoo JJ. Simultaneous analysis of anionic, amphoteric, nonionic and cationic surfactant mixtures in shampoo and hair conditioner by RP-HPLC/ELSD and LC/MS. Anal Chim Acta. 2008; 619: 129-136.

19. Hseu Z. Evaluating heavy metal contents in mine compost using four digestion methods. Biores Technol. 2004; 95: 53-59.

20. Chaturvedi V, Kumar A. Toxicity of sodium dodecyl sulfate in fishes and animals. Int J Appl Biol Pharm Technol. 2010; 2: 630-633.

21. Shukor MY, Husin WS, Rahman MF, Shamaan NA, Syed MA. Isolation and characterization of an SDS-degrading Klebsiella oxytoca. J Environ Biol. 2009; 30: 129-134.

22. Chukwu LO, Odunzeh CC. Relative toxicity of spent lubricant oil and detergent against benthic macro-invertebrates of a West African estuarine lagoon. J Environ Biol. 2006; 27: 479-484.

23. Liwarska-Bizukojc E, Miksch K, Jutsz AM, Kalka J. Acute toxicity and genotoxicity of five selected anionic and non-ionic surfactants. Chemosphere. 2005; 58: 1249-1253

24. Kumar M, Trivedi SP, Misra A, Sharma S. Histopathological changes in testis of the freshwater fish, Heteropneustes fossilis (Bloch) exposed to linear alkyl benzene sulphonate (LAS). J Environ Biol. 2007; 28: 679-684.

25. Shahbazi R, Kasra-Kermanshahi R, Gharavi S, Moosavi- Nejad Z, Borzooee F. Screening of SDS-degrading bacteria from car wash wastewater and study of the alkylsulfatase enzyme activity. Iran J Microbiol. 2013; 5(2): 153-155.

26. Hosseini F, Malekzadeh F, Amirmozafari N, Ghaemi N. Biodegradation of anionic surfactants by isolated bacteria from activated sludge. Int J Environ Sci Technol. 2007; 4(1): 127-132.

27. Schleheck D, Dong W, Denger K, Heinzle E, Cook AM. α-Proteobacterium converts linear alkylbenzenesulfonate surfactants into sulfophenyl carboxylates and linear alkyldiphenyletherdisulfonate surfactants into sulfodiphenyl-ethercarboxylates. Appl Environ Microbiol. 2000; 66: 1911-1916.

28. Singh VK, Xiong A, Usgaard TR, Chakrabarti S, Deora R, Misra TK, Jayaswal RK. ZntR is an autoregulatory protein and negatively regulates the chromosomal zinc resistance operon znt of Staphylococcus aureus. Mol Microbiol. 1999; 33: 200-207.

29. Sigoillot J, Nguyen M, Complete oxidation of linear alkyl benzene sulfonate bacterial communities selected from coastal seawater. Appl Environ Microbiol. 1992; 58: 1308-1312.

30. Rusnam M, Gusmanizar N. Characterization of the Growth on SDS by Enterobacter sp. strain. J Biochem Microbiol Biotechnol. 2017; 5(2): 28-32.
How to Cite
Adekanmbi, A.; Oyeladun, W.; Olaposi, A. Degradation of Surfactant and Metal-Removal by Bacteria from a Nigerian Laundry Environment. European Journal of Biological Research 2018, 8, 243-251.
Research Articles