Automated particle counting app in liquid medium based on digital image processing
Keywords:
App, Computer Vision, Counting, Particles, Micelles
Abstract
This work introduces the mobile app ContLab with advanced computational code installed on mobile devices (tablet, cellphone, iPad, etc.), with internet access, and compatible with Android operating systems. It is designed to be coupled with a simple microscope for the automated counting of particles, ranging in size from 0.05 µm to 50 µm, within the microfiltration spectrum, and specifically customized for micelle counting produced in fermentable processes. It is an easily accessible and cost-effective device for users interested in micelle counting present in any fermentable broth, such as in the pharmaceutical, food, and brewing industries. The app enhances the speed and precision of results compared to the manual counting method.
Downloads
Download data is not yet available.
References
1. Liu H, Li Z, Wang C, Zhang Q. Impacts of inflow conditions on the measurement stability of a turbulence-mitigation based liquid concentration detection system. Flow Measur Instrument. 2023; 90: 102337-102337.
2. Koppanen M, Kesti T, Rintala J, Marja RTP. Can online particle counters and electrochemical sensors distinguish normal periodic and aperiodic drinking water quality fluctuations from contamination? Sci Total Environt. 2023; 872: 162078-162078.
3. Bäuerlein PS, Pieke EN, Oesterholt FIHM, Laak T, Kools SAE. Microplastic discharge from a wastewater treatment plant: long term monitoring to compare two analytical techniques, LDIR and optical microscopy while also assessing the removal efficiency of a bubble curtain. Water Sci Technol. 2022; 87(1): 39-56.
4. Lu L, He Y, Zhang H, Chi J, Alexis KHL. Enhancing IAQ, thermal comfort, and energy efficiency through an adaptive multi-objective particle swarm optimizer-grey wolf optimization algorithm for smart environmental control. Building Environ. 2023; 235: 110235-110235.
5. Jia Z, Li J, Gao L, Yang D, Kanaev A. Dynamic light scattering: a powerful tool for in situ nanoparticle sizing. Colloids Interfaces. 2023; 7(1): 15.
6. Nowak ŁJ, Lankheet MJM. Understanding and optimizing fish counting techniques based on electrical impedance measurements. PLOS ONE. 2023; 18(10): e0293699-e0293699.
7. Lin M, Bhakdi S, Tan D, Jie J, Tai D, Pavesi A, et al. Lytic efficiency of immunosuppressive drugs resistant armored T cells against circulating HBV-related HCC in whole blood. Immunotherapy advances. 2023, 3(1): ltad015.
8. Guo Y, Li C, Zhao H, Gao M, Wang Q. The performance of microfiltration process for purifying lactic acid in the fermented broth of kitchen waste. Membranes. 2023; 13(3): 280-280.
9. Bertan AS, Cremasco MA. Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber. Eur J Biol Res. 2020; 10(3): 263-270.
10. Nguyen TT, Kamyingkird K, Phimpraphai W, Inpankaew T. Viability of Toxoplasma gondii tachyzoites in different conditions for parasite transportation. Vet World. 2022; 198-204.
11. Neves K, Menezes D, Rayêe D, Valério-Gomes B, Meneses P, Lent R, Mota B. The reliability of the isotropic fractionator method for counting total cells and neurons. J Neurosci Methods. 2019; 326: 108392.
12. Giechaskiel B. Particle number emissions of a diesel vehicle during and between regeneration events. Catalysts. 2020; 10(5): 587.
13. Ogunsola OT, Wang J, Song L. Survey of particle production rates from process activities in pharmaceutical and biological cleanrooms. Sci Technol Built Environ. 2019; 25(6): 692-704.
14. Bertan AS, Silva SCM, Cremasco MA. Production of tacrolimus using coconut oil as an alternative to glucose. Acta Scient Technol. 2021: e55721.
15. Pasternak AL, Park JM, Pai MP. Predictive Capacity of Population Pharmacokinetic Models for the Tacrolimus Dose Requirements of Pediatric Solid Organ Transplant Recipients. Therap Drug Monit. 2023; 45(1): 95-101.
16. Salava A, Perälä M, Pelkonen A, Mäkelä M, Remitz A. Safety of tacrolimus 0.03% and 0.1% ointments in young children with atopic dermatitis: a 36‐month follow‐up study. Clin Exp Dermatol. 2022; 47(5): 889-902.
17. ChauYee N, Peng-Chieh S, Tsung-Fu T, Yi-Jing L, Tzu-Li L. From zero to one: recent advances in the pathogenesis, diagnosis, and treatment of vitiligo. Dermatol Sinica. 2023; 41(3): 133.
18. Nie C, Luan W, Chen X, Li L, Ke W, Qiu M, Fan Y. Comparison of ceramic microfiltration and ultrafiltration membranes for the clarification of simulated sebacic acid fermentation broth. J Environ Chem Engin. 2023; 11(3): 109820-109820.
19. Long M, Cai Y, Zheng N, Lu Z, Cao W, Li Y, et al. Clean monascus pigments production from chinese rice wine wastes through submerged fermentation. Food Biosci. 2023; 52: 102451.
20. Su X, Qu J, Huang Y, Liu W, Sun Y. Enhancing biogas production of corn stover by biogas slurry reflux based on microfiltration membrane filtration and biochar adsorption. Agriculture. 2022; 12(12): 2040-2040.
21. Reis ME, Toledo AF, Silva AP, Poczynek M, Cantor MC, Virgínío JGF, et al. Effect of supplementation with algae β-glucans on performance, health, and blood metabolites of holstein dairy calves. J Dairy Sci. 2022; 105(10): 7998-8007.
22. Malafaia G, Luz TM, Araújo APC, Ahmed MAI, Rocha-Santos T, Barceló D. Novel methodology for identification and quantification of microplastics in biological samples. Environ Pollut. 2022; 292: 118466.
23. Patel BB, Rana PH. Performance evaluation of hybrid upflow anaerobic sludge blanket reactors with different inert media for sewage treatment. Biores Technol Rep. 2022; 18: 101075.
24. Patchett BD, Anderson BE. Nonlinear characteristics of high amplitude focusing using time reversal in a reverberation chamber. J Acoustical Soc Am. 2022; 151(6): 3603-3614.
25. Vásquez-Sepúlveda S, Guerrero N, Lemus CG, Meynard MM, Cerda M, Concha ML, et al. Protocol for extracting live blastoderm cells from embryos of annual killifish. Star Protocols. 2023; 4(3): 102344-102344.
26. Casali CI, Moreira A, Santacreu BJ, Pescio LG, Bonofiglio L, Romero DJ, Favale NO. Virtual microscope: using simulated equipment to teach experimental techniques and processes. Springer eBooks. 2022; 193-211.
27. Salguero J, Rendón MR, Valencia JT, Andrés J, Andrés C, Londoño OM, et al. Automatic detection of Cryptosporidium in optical microscopy images using YOLOv5x: a comparative study. Biochem Cell Biol. 2023; 101(6): 538-549.
28. Bertan AS. Microfiltration of micelles from fermentation of Streptomyces tsukubaensis for the production of tacrolimus. 2021. 129 p. Dissertation (Master’s Degree in Chemical Engineering) - University of Campinas, School of Chemical Engineering, Campinas, SP, 2021.
2. Koppanen M, Kesti T, Rintala J, Marja RTP. Can online particle counters and electrochemical sensors distinguish normal periodic and aperiodic drinking water quality fluctuations from contamination? Sci Total Environt. 2023; 872: 162078-162078.
3. Bäuerlein PS, Pieke EN, Oesterholt FIHM, Laak T, Kools SAE. Microplastic discharge from a wastewater treatment plant: long term monitoring to compare two analytical techniques, LDIR and optical microscopy while also assessing the removal efficiency of a bubble curtain. Water Sci Technol. 2022; 87(1): 39-56.
4. Lu L, He Y, Zhang H, Chi J, Alexis KHL. Enhancing IAQ, thermal comfort, and energy efficiency through an adaptive multi-objective particle swarm optimizer-grey wolf optimization algorithm for smart environmental control. Building Environ. 2023; 235: 110235-110235.
5. Jia Z, Li J, Gao L, Yang D, Kanaev A. Dynamic light scattering: a powerful tool for in situ nanoparticle sizing. Colloids Interfaces. 2023; 7(1): 15.
6. Nowak ŁJ, Lankheet MJM. Understanding and optimizing fish counting techniques based on electrical impedance measurements. PLOS ONE. 2023; 18(10): e0293699-e0293699.
7. Lin M, Bhakdi S, Tan D, Jie J, Tai D, Pavesi A, et al. Lytic efficiency of immunosuppressive drugs resistant armored T cells against circulating HBV-related HCC in whole blood. Immunotherapy advances. 2023, 3(1): ltad015.
8. Guo Y, Li C, Zhao H, Gao M, Wang Q. The performance of microfiltration process for purifying lactic acid in the fermented broth of kitchen waste. Membranes. 2023; 13(3): 280-280.
9. Bertan AS, Cremasco MA. Determination of concentration of the pseudomycelles from fermentation broth via spectroscopy and Neubauer chamber. Eur J Biol Res. 2020; 10(3): 263-270.
10. Nguyen TT, Kamyingkird K, Phimpraphai W, Inpankaew T. Viability of Toxoplasma gondii tachyzoites in different conditions for parasite transportation. Vet World. 2022; 198-204.
11. Neves K, Menezes D, Rayêe D, Valério-Gomes B, Meneses P, Lent R, Mota B. The reliability of the isotropic fractionator method for counting total cells and neurons. J Neurosci Methods. 2019; 326: 108392.
12. Giechaskiel B. Particle number emissions of a diesel vehicle during and between regeneration events. Catalysts. 2020; 10(5): 587.
13. Ogunsola OT, Wang J, Song L. Survey of particle production rates from process activities in pharmaceutical and biological cleanrooms. Sci Technol Built Environ. 2019; 25(6): 692-704.
14. Bertan AS, Silva SCM, Cremasco MA. Production of tacrolimus using coconut oil as an alternative to glucose. Acta Scient Technol. 2021: e55721.
15. Pasternak AL, Park JM, Pai MP. Predictive Capacity of Population Pharmacokinetic Models for the Tacrolimus Dose Requirements of Pediatric Solid Organ Transplant Recipients. Therap Drug Monit. 2023; 45(1): 95-101.
16. Salava A, Perälä M, Pelkonen A, Mäkelä M, Remitz A. Safety of tacrolimus 0.03% and 0.1% ointments in young children with atopic dermatitis: a 36‐month follow‐up study. Clin Exp Dermatol. 2022; 47(5): 889-902.
17. ChauYee N, Peng-Chieh S, Tsung-Fu T, Yi-Jing L, Tzu-Li L. From zero to one: recent advances in the pathogenesis, diagnosis, and treatment of vitiligo. Dermatol Sinica. 2023; 41(3): 133.
18. Nie C, Luan W, Chen X, Li L, Ke W, Qiu M, Fan Y. Comparison of ceramic microfiltration and ultrafiltration membranes for the clarification of simulated sebacic acid fermentation broth. J Environ Chem Engin. 2023; 11(3): 109820-109820.
19. Long M, Cai Y, Zheng N, Lu Z, Cao W, Li Y, et al. Clean monascus pigments production from chinese rice wine wastes through submerged fermentation. Food Biosci. 2023; 52: 102451.
20. Su X, Qu J, Huang Y, Liu W, Sun Y. Enhancing biogas production of corn stover by biogas slurry reflux based on microfiltration membrane filtration and biochar adsorption. Agriculture. 2022; 12(12): 2040-2040.
21. Reis ME, Toledo AF, Silva AP, Poczynek M, Cantor MC, Virgínío JGF, et al. Effect of supplementation with algae β-glucans on performance, health, and blood metabolites of holstein dairy calves. J Dairy Sci. 2022; 105(10): 7998-8007.
22. Malafaia G, Luz TM, Araújo APC, Ahmed MAI, Rocha-Santos T, Barceló D. Novel methodology for identification and quantification of microplastics in biological samples. Environ Pollut. 2022; 292: 118466.
23. Patel BB, Rana PH. Performance evaluation of hybrid upflow anaerobic sludge blanket reactors with different inert media for sewage treatment. Biores Technol Rep. 2022; 18: 101075.
24. Patchett BD, Anderson BE. Nonlinear characteristics of high amplitude focusing using time reversal in a reverberation chamber. J Acoustical Soc Am. 2022; 151(6): 3603-3614.
25. Vásquez-Sepúlveda S, Guerrero N, Lemus CG, Meynard MM, Cerda M, Concha ML, et al. Protocol for extracting live blastoderm cells from embryos of annual killifish. Star Protocols. 2023; 4(3): 102344-102344.
26. Casali CI, Moreira A, Santacreu BJ, Pescio LG, Bonofiglio L, Romero DJ, Favale NO. Virtual microscope: using simulated equipment to teach experimental techniques and processes. Springer eBooks. 2022; 193-211.
27. Salguero J, Rendón MR, Valencia JT, Andrés J, Andrés C, Londoño OM, et al. Automatic detection of Cryptosporidium in optical microscopy images using YOLOv5x: a comparative study. Biochem Cell Biol. 2023; 101(6): 538-549.
28. Bertan AS. Microfiltration of micelles from fermentation of Streptomyces tsukubaensis for the production of tacrolimus. 2021. 129 p. Dissertation (Master’s Degree in Chemical Engineering) - University of Campinas, School of Chemical Engineering, Campinas, SP, 2021.
Published
2024-08-29
How to Cite
(1)
Suzin Bertan, A.; Suzin Bertan, L.; Cremasco, M. Automated Particle Counting App in Liquid Medium Based on Digital Image Processing. European Journal of Biological Research 2024, 14, 64-75.
Section
Research Articles
This work is licensed under a Creative Commons Attribution 4.0 International License.
