Antibiotic resistance and virulence genes in Campylobacter species from pig and cattle samples in Ibadan, Nigeria

  • Olutayo Israel Falodun Department of Microbiology, University of Ibadan, Ibadan, Nigeria
  • Odunsanmi Ajibodun Waleola Department of Microbiology, University of Ibadan, Ibadan, Nigeria
Keywords: Campylobacteriosis, cadF genes, hipO genes.

Abstract

Campylobacter species are the leading cause of gastroenteritis worldwide with incidence cases higher than those caused by Salmonella. This study was designed to determine the antibiotic resistance patterns and virulence genes detection in selected Multi-Drug Resistant (MDR) strains of Campylobacter species isolated from pigs and cattle dungs. Stool samples were collected from pigs and cattle at the teaching and research farm of the University of Ibadan, Nigeria. Isolation and identification of Campylobacter species were made using modified charcoal cefoperazone deoxycholate agar and standard biochemical tests. Antibiotic susceptibility test was carried out using the disk diffusion technique. The hipO and cadF virulence genes were detected using a multiplex-polymerase chain reaction. The occurrence of Campylobacter species in pigs and cattle was 90.0% and 95.0%, respectively. In pigs, C. coli had the highest occurrence, while it was C. jejuni in cattle. The antibiotic resistance patterns showed that 1.3% and 1.2% of the isolates from pigs and cattle, respectively were resistant to all the antibiotics tested. Among the selected MDR strains, cadF genes were detected in 76.5% (pigs) and 75.0% (cattle). However, hipO genes were detected in 11.8% (pigs) and 50.0% (cattle) isolates. The cadF gene was detected in all the Campylobacter species, while hipO gene was detected only in C. jejuni. In conclusion, the pigs and cattle faecal wastes harbored virulent and multidrug-resistant Campylobacter species. Hence, the indiscriminate discharge of untreated animal faecal wastes into the environment and water bodies should be discouraged.

DOI: http://dx.doi.org/10.5281/zenodo.10909573

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Published
2024-04-02
How to Cite
(1)
Falodun, O.; Waleola, O. Antibiotic Resistance and Virulence Genes in Campylobacter Species from Pig and Cattle Samples in Ibadan, Nigeria. European Journal of Biological Research 2024, 14, 33-44.
Section
Research Articles