In vivo assessment of anti-inflammatory and antioxidant activities of Phlomis crinita polyphenols

  • Hanane Boutennoun Molecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria; Laboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria
  • Lilia Boussouf Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria; Applied Microbiology and Food Sciences Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria
  • Nassima Balli Molecular and Cell Biology Department, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria; Environment, Health and Biotechnology Laboratory, Faculty of Nature and Life Sciences, University of Jijel, 18000 Jijel, Algeria
  • Lila Boulekbache Makhlouf Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria
  • Khodir Madani Laboratory of Biomathematics, Biophysics, Biochemistry, and Scientometry Laboratory (L3BS), Faculty of Nature and Life Sciences, University of Bejaia, 06000 Bejaia, Algeria; Research Centre in Agro-Food Technologies, Targua Ouzemmour Road, 06000 Bejaia, Algeria
  • Khaled Al-Qaoud Molecular Immunoparasitology Laboratory, Biological Sciences Department, Faculty of Sciences, University of Yarmouk, Irbid, Jordan
Keywords: Phlomis crinita, Phenolic content, Edema, Stress

Abstract

The leaves of Phlomis crinita are traditionally used in Algerian medicine for the treatment of pain and inflammatory conditions. In order to find a potential application for this native species, the anti-inflammatory and antioxidant effects were investigated on various in vivo experimental models, and the total phenolic compounds and flavonoid contents were determined. The carrageenan-induced paw edema method was used to evaluate the anti-inflammatory activity of the extract in vivo, while the in vivo antioxidant effect was assessed by estimating oxidative stress parameters (MDA, CAT, and SOD). Phytochemical screening revealed the presence of substances with high therapeutic values. In vivo anti-inflammatory studies show that plant extract has a significant and dose-dependent impact on the inhibition of edema formation. The maximum percentage inhibition value was 87.79% after 4 h at a concentration of 500 mg/kg. Moreover, the administration of the extract significantly enhanced the activities of antioxidant enzymes in the livers of mice. It significantly (p ˂ 0.05) increased CAT and SOD activities and significantly (p ˂ 0.05) decreased the MDA level activity, compared to the control inflammatory group. Our findings support that Phlomis crinita can be considered as a promising source of therapeutic bioactive compounds.

DOI: http://dx.doi.org/10.5281/zenodo.7860334

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Published
2023-04-24
How to Cite
(1)
Boutennoun, H.; Boussouf, L.; Balli, N.; Makhlouf, L.; Madani, K.; Al-Qaoud, K. In Vivo Assessment of Anti-Inflammatory and Antioxidant Activities of Phlomis Crinita Polyphenols. European Journal of Biological Research 2023, 13, 94-105.
Section
Research Articles