In vitro studies of iron absorption and activity of glutathione peroxidase in intestinal mucosa of the chicken

  • Jurijs Markovs Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
  • Agate Galuza Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
  • Natalija Basova Institute of Biology of the University of Latvia, Riga, Latvia
  • Gundega Knipse Department of Anatomy and Histology, Faculty of Medicine, University of Latvia, Riga, Latvia
  • Svetlana Vasiljeva Institute of Biology of the University of Latvia, Riga, Latvia
  • Galina Smirnova Institute of Biology of the University of Latvia, Riga, Latvia
Keywords: Glutathione peroxidase, Iron absorption, Intestinal mucosa, Chicken


We examined the absorption of iron, the activity of selenoprotein glutathione peroxidase (GSH·Px) and cellular compartmentalization of metal in the chicken duodenum and ileum. The method of accumulating mucosa preparation (AMP) was used. It was shown that the intestinal iron accumulation is dose-dependent process, which has two components: transcellular and paracellular. The realization of these pathways is region-specific and depends on exposed iron levels. Slightly elevated iron status of intestinal mucosa does not influence activity of GSH·Px. At the same time the results indicate that the activity of glutathione peroxidase can be altered by iron overload. Immunohistochemistry revealed that stainable iron could be co-localized to the endolysosomal compartment. How the activity of enzyme can be affected by oxidative stress and competitive interactions of iron with selenium are discussed.



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How to Cite
Markovs, J.; Galuza, A.; Basova, N.; Knipse, G.; Vasiljeva, S.; Smirnova, G. In Vitro Studies of Iron Absorption and Activity of Glutathione Peroxidase in Intestinal Mucosa of the Chicken. European Journal of Biological Research 2018, 8, 168-173.
Research Articles