Neurospora tetraspora D. Garcia, Stchigel & Guarro (= Gelasinospora tetrasperma Dowding) as a first record to Egypt
Keywords:
Assiut, Grapevine, Genotypic and phenotypic characterization, ITS, Soil
Abstract
An interesting isolate of homothallic, ascosporic filamentous fungus having 4-spored asci, was recovered once from a non-rhizosphere soil sample collected from a grapevine plantation in the village of El-Khawaled, Sahel-Saleem city, Assiut. It was isolated on DYM agar plate at 25°C in June 2008. The isolate was identified phenotypically and genotypically as Neurospora tetraspora (= Gelasinospora tetrasperma) and was deposited in the culture collection of Assiut University Mycological Centre as AUMC no. 6784 and ITS gene sequence of the strain was deposited at the National Center for Biotechnology Information (NCBI) and assigned a GenBank accession number JQ425383. N. tetraspora is being recorded in the current work for the first time in Egypt. By this addition, the genus is now known in Egypt by four species. A key is provided for the four species.
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References
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40. Wang XW, Lombard L, Groenewald JZ, Li J, Videira SI, Samson RA, et al. Phylogenetic reassessment of the Chaetomium globosum species complex. Persoonia. 2016; 36: 83-133.
2. Moubasher AH, Abdel-Sater MA, Soliman Z. Biodiversity and molecular characterization of yeast and filamentous fungi in the air of citrus and grapevine plantations in Assiut area, Egypt. Mycosphere. 2016; 7(3): 236-261.
3. Shear CL, Dodge BO. Life histories and heterothallism of the red bread-mold fungi of the Monilia sitophila group. J Agricult Res. 1927; 34: 1019-1042.
4. Cain RF. Studies of coprophilous ascomycetes. I. Gelasinospora. Can J Res. 1950; 28: 566-576.
5. von Arx JA. A key to the species of Gelasinospora. Persoonia. 1982; 11: 443-449.
6. Garcia D, Stchigel AM, Cano J, Guarro J, Hawksworth DL. A synopsis and re-circumscription of Neurospora (syn. Gelasinospora) based on ultrastructural and 28S rDNA sequence data. Mycol Res. 2004; 108(10): 1119-1142.
7. Tai FL. Two new species of Neurospora. Mycologia. 1935; 27: 328-330.
8. Gochenaur SE, Backus MP. A new species of Neurospora from Wisconsin lowland soil. Mycologia.1962; 54: 555-562.
9. Nelson AC, Novak RO, Backus MP. A new species of Neurospora from soil. Mycologia. 1964; 56: 384-392.
10. Frederick LF, Uecher FA, Benjamin CP. A new species of Neurospora from soil of West Pakistan. Mycologia. 1969; 61: 1077-1084.
11. Mahoney DP, Huang LH, Backus MP. New homothallic Neurosporas from tropical soils. Mycologia. 1969; 61: 264-272.
12. von Arx JA. The genera of fungi sporulating in pure culture. J. Cramer, Vaduz, 3rd edn, 1981.
13. Perkins DD, Raju NB. Neurospora discreta, a new heterothallic species defined by its crossing behaviour. Exp Mycology. 1986; 10: 323-338.
14. Krug JC, KHAN RS. A new homothallic species of Neurospora from Hungary. Mycologia. 1991; 83: 829-832.
15. Dowding ES. Gelasinospora, a new genus of Pyrenomycetes with pitted spores. Can J Res. 1933; 9: 294-305.
16. Moreau C, Moreau M. Quelques ascomycetes du Congo. Revue de Mycologie. 1949; 2: 50-66.
17. Udagawa S, Takada M. Notes on some Japanese ascomycetes. Transact Mycol Soc Japan. 1967; 8: 50-53.
18. Cailleux R. Recherches sur la mycoflore coprophile centrafricaine. Le genres Sordaria, Gelasinospora, Bombardia. Bull Soc Mycol France. 1971; 87: 461-626
19. Udagawa S, Furuya K, Horie Y. Mycological reports from New Guinea and the Solomon Islands. 19. Notes on some ascomycetous microfungi from soil. Bull Nat Sci Museum Tokyo. 1973; 16: 503-520.
20. Horie Y, Udagawa S. Anixiella and Gelasinospora from Himalayan soils. Transact Mycolog Soc Japan. 1974; 15: 196-205.
21. Furuya K, Udagawa S. New species of Gelasinospora and Anixiella. Transact Mycol Soc Japan. 1976; 17: 313-320.
22. Khan RS, Krug JC. New species of Gelasinospora. Mycologia. 1989; 81: 226-233.
23. Khan RS, Krug JC. A new terricolous species of Gelasinospora. Sydowia. 1989; 41: 180-184.
24. Krug JC, Khan RS, Jeng RS. A new species of Gelasinospora with multiple germ pores. Mycologia. 1994; 86: 250-253.
25. Stchigel AM, Cano J, Guarro J. A new species of Gelasinospora from Argentinian soil. Mycol Res. 1998; 102: 1405-1408.
26. Dettman JR, Harbinski FM, Taylor JW. Ascospore morphology is a poor predictor of the phylogenetic relationships of Neurospora and Gelasinospora. Fungal Gen Biol. 2001; 34: 49-61.
27. Cai L, Jeewon R, Hyde KD. Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycol Res. 2006; 110: 137-150.
28. Moharram AM, Bagy MMK, Abdel-Mallek AY. Saprophytic fungi isolated from animal and bird pens in Egypt. J Basic Microbiol. 1987; 27(7): 361-367.
29. Moharram AM, Abdel-Gawad KM, Megalla SE, Mahmoud A-LE. Fungal flora of poultry feedstuff ingredients. J Basic Microbiol. 1989; 29(8): 491-499.
30. Abdel-Hafez SII, Moubasher AH, Shoreit AAM, Ismail MA. Fungal flora associated with combine harvester wheat and sorghum dusts from Egypt. J Basic Microbiol. 1990; 30(7): 467-479.
31. Abdel-Mallek AY, Abdel-Hafez AII, Moharram AM. Contribution to the mycoflora of caraway, coriander and cumin seeds in Egypt. Bull Faculty Sci Assiut Univ. 1990; 19(1-D): 1-15.
32. Ismail MA, Abdel-Hafez SII, Moharram AM. Aeromycobiota of western desert of Egypt. Afr J Sci Technol. 2002; 3(1): 1-9.
33. Abdel-Sater MA, Moubasher AH, Soliman ZSM. Biodiversity of filamentous and yeast fungi in soil of citrus and grapevine plantations in Assiut area, Egypt. Czech Mycol. 2016; 68(2): 183-214.
34. Moubasher AH, Moharram AM, Soliman Z. Contributions to the mycobiota of Egypt: Neurospora tetrasperma Shear & Dodge; a new record to Egypt. J Basic Appl Mycol Egypt. 2010; 1: 67-70.
35. Samson RA, Hoekstra ES, Frisvad JC. Introduction to food- and airborne fungi. 7th edn. Centraalbureauvoor Schimmelcultures, Utrecht, The Netherlands, 2004.
36. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673-4680.
37. Ginns JH. Compendium of plant disease and decay fungi in Canada 1960-1980. Agricultur Canada, Ottawa, 1986.
38. Lumley TC, Abbott SB, Currah RS. Microscopic ascomycees isolated from rotting wood in the Boreal forest. Mycotaxon. 2000; 74(2): 395-414.
39. Geydan TD, Debets AJ, Verkley GJ, van Diepeningen AD. Correlated evolution of senescence and ephemeral substrate use in the Sordariomycetes. Mol Ecol. 2012; 21(11): 2816-2828.
40. Wang XW, Lombard L, Groenewald JZ, Li J, Videira SI, Samson RA, et al. Phylogenetic reassessment of the Chaetomium globosum species complex. Persoonia. 2016; 36: 83-133.
Published
2018-02-14
How to Cite
(1)
Moubasher, A.; Abdel-Sater, M.; Soliman, Z. Neurospora Tetraspora D. Garcia, Stchigel & Guarro (= Gelasinospora Tetrasperma Dowding) As a First Record to Egypt. European Journal of Biological Research 2018, 8, 14-20.
Section
Research Articles
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