Antioxidant activity of extracts formulated from Citrus aurantium and Artemisia herba alba
Abstract
Plants still present a large source of novel active biological compounds with different activities. The antioxidant activity of three extracts was evaluated by DPPH and ferric reducing antioxidant power methods. The formulated extracts were analyzed by thin layer chromatography (TLC) and then confirmed by High performance liquid chromatography coupled with DAD detector (HPLC-DAD). The results showed the richness of these extracts in phenolic compounds. Three major compounds, resveratrol (17.98%), kaempferol-glucoside (7.23%) and vanillic acid (10.64%) were detected in methanolic extract of Citrus aurantium peel, aqueous extract of Citrus aurantium L. leaves and ethanolic extract of Artemisia herba alba Asso respectively by HPLC-DAD. However, the ethanolic extract of A. herba alba achieved 50% of the anti-radical activity at a concentration equal to 0.8 mg/ml. A higher antioxidant activity measured by ferric reducing antioxidant power was marked in the same extract with an absorbance equal to 0.824. The ethanolic extract of the aerial part of A. herba alba, the methanolic extract of C. aurantium peel and the aqueous extract of C. aurantium leaves were considered as powerful scavengers of free radicals and can be incorporated into the pharmaceuticals preparations to treat many diseases.
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