Heavy metals biosorption by urease producing Lysinibacillus fusiformis 5B

  • Amina Musa Jibrin Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
  • Oluwafemi Adebayo Oyewole Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
  • Japhet Gaius Yakubu Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
  • Aisha Hussaini Department of Microbiology, Federal University of Technology Minna, Niger State, Nigeria
  • Evans Chidi Egwim Department of Biochemistry, Federal University of Technology Minna, Niger State, Nigeria
Keywords: Lysinibacillus fusiformis 5B, Biosorption, Cadmium, Chromium, Lead, Nickel


Biosorption is the ability of biological materials to accumulate heavy metals from wastewater through mediated or physico-chemical pathways of uptake. Urease producing bacteria have been hypothesized to have inherent bioremediation abilities. The aim of this research was to determine the potential of Lysinibacillus fusiformis 5B to biosorp Pb, Cr, Cd and Ni. The stock solution of Pb, Cr, Cd and Ni was prepared by dissolving 0.0157 g of Pb(C2H3O2)2, 0.057 g of K2Cr2O7, 0.018 g of CdSO4 and 0.026 g of NiSO4 in 100 mL of dH2O respectively. Lysinibacillus fusiformis 5B was screened for the potential to utilise 5 ppm of the heavy metals using agar dilution method. Broth of L. fusiformis 5B was inoculated to 10, 15, 20 and 50 ppm of the heavy metals. The rate of biosorption was determined by atomic absorption spectroscopy (AAS) after 0, 7, 14, 21, 28 and 35 days. The biosorption % was determined by Beer Lambart’s equation. Lysinibacillus fusiformis 5B was able to tolerate 5 ppm concentration of all the heavy metals by showing visible growth on surfaces of nutrient agar Petri plates. Generally, there was an increase in biosorption rate as the days progress. After 35 days of incubation, the highest biosorption rate of 99.96%, 99.97%, and 99.94% were recorded for Pb, Cr, and Cd respectively at 10 ppm and 99.33% of Ni at 15 ppm. The results of this study showed that L. fusiformis 5B possess the capacity to biosorp Pb, Cr, Cd and Ni and can be developed as biosorption agent for these heavy metals.

DOI: http://dx.doi.org/10.5281/zenodo.4023161


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1. Jacob JM, Karthik C, Saratale RG, Kumar SS, Prabakar DP, Kadirvelu K, Pugazhendhi A. Biological approaches to tackle heavy metal pollution: A survey of literature. J Environ Manag, 2018; 217: 56-70.

2. Calderón OAR, Abdeldayem OM, Pugazhendhi A, Rene ER. Current updates and perspectives of biosorption technology: an alternative for the removal of heavy metals from wastewater. Curr Pollut Rep. 2020; 6: 8-27.

3. Abbas SH, Ismail IM, Mostafa TM, Sulaymon AH. Biosorption of heavy metals: a review. Int J Chem Sci. 2014; 3(4): 74-102.

4. Choudhary M, Kumar R, Datta A, Nehra V, Garg N. Bioremediation of heavy metals by microbes. Bioremediation of salt affected soils: an Indian perspective. Springer Nature Publishing. 2017; 233-235.

5. Yin K, Wanga Q, Lva M, Chena L. Microorganism remediation strategies towards heavy metals. Chem Eng J. 2018; 360: 1553-1563.

6. Oyewole OA, Zobeashia SSL, Oladoja EO, Raji RO, Odiniya EE, Musa AM. Biosorption of heavy metal polluted soil using bacteria and fungi isolated from soil. SN Appl Sci. 2019; 1: 857.

7. Aryal M. A comprehensive study on the bacterial biosorption of heavy metals: materials, performances, mechanisms, and mathematical modellings. Rev Chem Eng. 2020; DOI: 10.1515/revce-2019-0016.

8. Muhammad LH, Ibrahim MB. Biosorption of iron by heavy metal tolerant Micrococcus sp. Bayero J Pure Appl Sci. 2018; 11(1): 102-109.

9. Ramya D, Thatheyus JA. Microscopic investigations on the biosorption of heavy metals by bacterial cells: a review. Sci Int. 2018; 6: 11-17.

10. Bhutada SA, Dahikar SB. Evaluation of removal of heavy metals by microorganisms isolated from industrial effluents. J Appl Adv Res. 2017; 2(3): 156-160.

11. Kanamarlapudi SLRK, Chintalpudi VK, Muddada S. Application of biosorption for removal of heavy metals from wastewater. IntechOpen, 2018.

12. Olawale SA. Biosorption of heavy metals: a mini review. Acta Sci Agric. 2019; 3(2): 22-25.

13. Ghosh A, Dastidar MG, Sreekrishnan TR, Asce AM. Recent advances in bioremediation of heavy metals and metal complex dyes: review. J Environ Eng. 2015; DOI: 10.1061/(ASCE)EE.1943-7870.0000965.

14. Sharma S, Tiwari S, Hasan A, Saxena V, Pandey LM. Recent advances in conventional and contemporary methods for remediation of heavy metal‑contaminated soils. 3 Biotech. 2018; 8: 216.

15. Farnane M, Machrouhi A, Elhalil A, Abdennouri M, Qourzal S, Tounsadi H, Barka N. New sustainable biosorbent based on recycled deoiled carob seeds: optimization of heavy metals remediation. J Chem. 2018; ID 5748493.

16. Utomo HD, Tan KXD, Choong ZYD, Yu JJ, Ong JJ, Lim BZ. Biosorption of heavy metal by algae biomass in surface water. J Environ. 2016; 7: 1547-1560.

17. Dewi NK, Mubarok I, Yuniastuti A. Biosorption of heavy metal pollution by Enterobacter agglomerans. Biosaintifika. 2019; 11(2): 289-295.

18. Abioye OP, Oyewole OA, Oyeleke SB, Adeyemi MO, Orukotan AA. Biosorption of lead, chromium and cadmium in tannery effluent using indigenous microorganisms. Braz J Biol Sci. 2018; 5(9): 25-32.

19. Helmy OT, Abou-Taleb KA, Abdel-Monem MO, Abd El-salam SS. Isolation and evaluation of the tolerance of industrial wastewater bacteria to heavy metals toxicity. AASCIT J Biol. 2018; 4(2): 25-34.

20. Azubuike CC, Chikere CB, Okpokwasili GC. Bioremediation techniques–classification based on site of application: principles, advantages, limitations and prospects. World J Microb Biot. 2016; 32: 180.

21. Verma N, Sharma R. Bioremediation of toxic heavy metals: a patent review. Recent Pat Biotechnol. 2017; 11(2): 1-17.

22. Ojuederie OB, Babalola OO. Microbial and plant-assisted bioremediation of heavy metal polluted environments: a review. Int J Env Res Pub He, 2017; 14: 1504.

23. Mathivanan K, Rajaram R, Annadurai G. Biosorption potential of Lysinibacillus fusiformis KMNTT-10 biomass in removing lead(II) from aqueous solutions. Sep Sci Technol. 2018; 53(13): 1991-2003.

24. Ansari MI, Masood F, Malik A. Bacterial biosorption: a technique for remediation of heavy metals. Agric Environ Appl. 2011; 283-316.

25. Divakar K, Prabha S, Gautam P. Purification, immobilization and kinetic characterization of G-x-S-x-G esterase with short chain fatty acid specificity from Lysinibacillus fusiformis AU01. Biocatal Agric Biotechnol. 2017; 12: 131-141.

26. Kumari D, Qian XY, Pan X, Achal V, Li Q, Gadd GM. Microbially-induced carbonate precipitation for immobilization of toxic metals. Adv Appl Microbiol. 2016; 94: 79-108.

27. Shamim S. Biosorption of heavy metals. IntechOpen, 2018.

28. Maity JP, Chen GS, Huang YH, Sun A, Chen C. Ecofriendly heavy metal stabilization: microbial induced mineral precipitation (MIMP) and biomineralization for heavy metals within the contaminated soil by indigenous bacteria. Geomicrobiol J. 2019; 36: 612-623.

29. He M, Li X, Liua H, Miller SJ, Wanga G, Rensing C. Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1. J Hazard Mater. 2011; 185: 682-688.

30. Raja CE, Omine K. Characterization of boron resistant and accumulating bacteria Lysinibacillus fusiformis M1, Bacillus cereus M2, Bacillus cereus M3, Bacillus pumilus M4 isolated from former mining site, Hokkaido, Japan. J Environ Sci Heal A. 2012; 47(10): 1341-1349.

31. Saurabh G, Goyal R, Nirwan J, Cameotra SS, Tejoprakash N. Biosequestration, transformation, and volatilization of mercury by Lysinibacillus fusiformis isolated from industrial effluent. J Microbiol Biotechnol. 2012; 22(5): 684-689.

32. Yan H, Han Z, Zhao H, Pan J, Zhao Y, Tucker ME, Fan D. The bio-precipitation of calcium and magnesium ions by free and immobilized Lysinibacillus fusiformis DB1-3 in the wastewater. J Clean Prod. 2020; 252: 119826.

33. Alex R. Bioremediation of Hydrocarbons by Lysinibacillus fusiformis BTTS10. A Thesis for Doctor of Philosophy (Ph.D) in Biotechnology submitted to Department of Biotechnology, Cochin University of Science and Technology, 2012.

34. Verma S, Kuila A. Bioremediation of heavy metals by microbial process. Environ Technol Innov. 2019; 14: 100369.

35. Kranthi RK, Sardara UR, Bhargavib E, Devic I, Bhuniad B, Tiwarie ON. Advances in exopolysaccharides based bioremediation of heavy metals in soil and water: a critical review. Carbohydr Polym. 2018; 199: 353-364.
How to Cite
Jibrin, A.; Oyewole, O.; Yakubu, J.; Hussaini, A.; Egwim, E. Heavy Metals Biosorption by Urease Producing Lysinibacillus Fusiformis 5B. European Journal of Biological Research 2020, 10, 326-335.
Research Articles