Efficacy of octenidine against Pseudomonas aeruginosa strains

  • Tomasz M. Karpiński Department of Medical Microbiology, Poznań University of Medical Sciences, Wieniawskiego 3, 61-712 Poznań, Poland https://orcid.org/0000-0001-6599-9204
Keywords: Pseudomonas aeruginosa, Octenidine dihydrochloride, Octenisept, Antibacterial activity, TTC, MTT


Pseudomonas aeruginosa is a Gram-negative bacterium causing skin and soft tissue infections, complicated urinary tract infections, blood infections, and nosocomial (hospital-acquired) infections. One of the most often used antiseptics in the skin and soft tissue infections is octenidine dihydrochloride. The aim of this study was an evaluation of octenidine activity against strains of P. aeruginosa. Additionally, were compared two staining methods (TTC and MTT) for confirmation of bacterial growth. The study involved eight strains of P. aeruginosa. In order to determine the minimum inhibitory concentration (MIC) of octenidine, the microdilution method was used. For bacterial growth detection was used staining method with 2,3,5-triphenyl-tetrazolium chloride (TTC) and with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). In the study has been demonstrated the excellent activity of octenidine against all strains of Pseudomonas aeruginosa. For all tested strains, MICs of octenidine were 0.00039% or 0.00078%, what is equivalent to 3.9 µg/ml and 7.8 µg/ml, respectively. In the study, test with MTT for three strains was more sensitive than a test with TTC. Concluding, octenidine is an antiseptic with high efficacy against Pseudomonas aeruginosa strains. Simultaneously, it was stated that a test with MTT is more sensitive than study with TTC.

DOI: http://dx.doi.org/10.5281/zenodo.3339499


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How to Cite
Karpiński, T. Efficacy of Octenidine Against Pseudomonas Aeruginosa Strains. European Journal of Biological Research 2019, 9, 135-140.
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