Efficiency of consortium for in-situ bioremediation and CO2 evolution method of refines petroleum oil in microcosms study

  • Shreyasri Dutta Department of Microbiology, Gurukul Kangri University (KGC), Haridwar, Uttrakhand-249404, India
  • Padma Singh Department of Microbiology, Gurukul Kangri University (KGC), Haridwar, Uttrakhand-249404, India
Keywords: In-situ bioremediation, CO2 evolution method, Microbial consortium, Pseudomonas aeruginosa, Aspergillus terrus, Aspergillus flavus


An in-situ bioremediation study was conducted in a laboratory by using mixed microbial consortium. An indigenous microbial consortium was developed by assemble of two Pseudomonas spp. and two Aspergillus spp. which were isolated from various oil contaminated sites of India. The laboratory feasibility study was conducted in a 225 m2 block. Six treatment options: Oil alone, Oil+Best remediater, Oil+Bacterial consortium, Oil+Fungal consortium, Oil+Mixed microbial consortium, Oil+Indigenous microflora. Out of five treatments, the mixed microbial consortium (Block 5) degraded 55.12% refine petroleum oil compare to degradation of bacterial (Block 3) and Fungal consortium (Block 4) (i.e, degradation rate were 19.88% and 18.07% correspondingly) after the end of treatment (60 days). Bioremediation ability of this consortium was confirmed by CO2 evolution method. The result showed that 136.36 % CO2 release after 12 days incubation. 16r DNA sequencing showed that two bacterial species were Pseudomonas aeruginosa and morph taxonomical examination of fungus were Aspergillus terrus (At) and Aspergillus flavus (Af).The ability of degradation of  synthetic mixture of refine petroleum oils  makes the consortium potentially useful for bioremediation and microbial enhanced oil recovery.

DOI: http://dx.doi.org/10.5281/zenodo.501069


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1. Salleh AB, Ghazali FM, Rahman RNZA, Basri M. Bioremediation of petroleum hydrocarbon pollution. Indian J Biotech. 2003; 2(7): 411-425.
2. Dutta S, Singh P. Chemotaxis of biofilm producing Pseudomonas spp. towards refined petroleum oil. J Sci Res. 2016; 8(2): 199-207.
3. Dutta S, Singh P. The Placket-Burman model-optimization of significant nutritional parameters for petroleum bioremediation by Pseudomonas sp. Int J Adv Res. 2014; 2(1): 898-902.
4. Dutta S, Singh P. Laboratory scale experiment on the bioremediation of refined petroleum hydro-carbon by using CO2 evolution method. Int J Basic Appl Biol. 2014; 1(1): 6-10.
5. Mariano AP, Kataoka APAG, Angelis DF, Banotto DM. Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station. Braz J Microbiol. 2007; 38: 2.
6. Mukred AM, Hamid AA, Hamzah A, Yusoff WMW. Development of three bacteria consortium for the bioremediation of crude petroleum oil in contaminated water. Online J Biol Sci. 2008; 8(4): 73-79.
7. Lemos JLS, Rizzo AC, Millioli VS, Soriano AU, Sarquis MIM, Santos R. Petroleum degradation by filamentous fungi. Environmental Conferences - IPEC - The University of Tulsa, 2002.
8. Malik S, Iftikhar T, Haq I. Process optimization for a potent wild and mutant strain of Aspergillus niger for biosynthesis of amyloglucosidsae. Pak J Bot. 2011; 43: 2211-2219.
9. Pritchard PH, Bourquin AW. The use of micro-cosms for evolution of interactions between pollutants and microorganisms. Adv Microb Ecol. 1984; 7: 133-215.
10. Mittal A, Singh P. A feasibility study for assessment of in-situ bioremediation potential of a crude oil degrading Pseudomonas consortium. J Sci Res. 2010; 2(1): 127-137.
11. Mittal A, Singh P. Polycyclic aromatic hydro-carbon degradation by developed consortium in microcosm’s study. Internet J Microbiol. 2008; 7: 1.
12. ASTM D 5864. In the U.S., the ASTM committee adopted the OECD 301-B modified Sturm procedures within ASTM D-5864-00 standard test method for determining aerobic aquatic biodegradation of lubricants, 1995.
13. Zhang XCL, Peterson D, Reese G, Möller, Haws R. Biodegradation of biodiesel in the aquatic environ-ment. Department of Agricultural Engineering, University of Idano, USA, 1995.
14. Verstraete W, Vanloocke R, DeBorger R, Verlindne A. Modeling of the breakdown and the mobilization of hydrocarbons in unsaturated soil layers. In: Proceedings of 3rd International Biodegradation Symposium. Sharpley JM, Kalpan AM, eds. Applied Science Publishers, London, 1975: 98-112.
15. Rahman KSM, Rahman JT, Lakshmanaperumal-samy P, Banat IM. Towards efficien crude oil degradation by a mixed bacterial consortium. Biores Technol. 2002; 85: 257-261.
16. Dutta S, Singh P. Hydrocarbon degradation potential of indigenous fungal isolates from Indian oil refinery, Haldia, (W.B), India. Sci Res Reporter. 2016; 6(1): 04-11.
17. Top EM, Springael D. The role of mobile genetic elements in bacterial adaptation to xenobiotic organic compounds. Curr Opin Biotechnol. 2003; 14(3): 262-269.
18. Muthuswamy S, Binupriya AR, Baik S, Yun S. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas. Clean Soil Air Water. 2008; 36: 92-96.
19. Vasudevan N, Rajaram P. Bioremediation of oil sludge contaminated soil. Environ Int. 2001; 26: 409-411.
20. Ozaki S, Kishimoto N, Fujita T. Change in the predominant bacteria in a microbia consortium cultured on media containing aromatic and saturated hydrocarbons as the so carbon source. Microbes Environ. 2007; 22: 128-135.
21. Hasanuzzaman M, Ueno A, Ito H, Ito Y, Yamamoto Y, Yumoto I, Okuyama H. Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG. Int Biodeter Biodegr. 2007; 59: 40-43.
22. Zhang GL, Yu YT, Qian XP, Meng Q. Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids. J Zhejiang Univ Sci. 2005; 6: 725-730.
23. Tzarkova EK, Groudeva VI. Bioremediation of petroleum-polluted soils by adsorptiv immobilized Corynebacterium sp. RB-96. Biotechnol Biotechn Equip. 2000; 14: 72-75.
24. Rahman KSM, Thahira-Rahman J, Kourkoutas Y, Petsas I, Marchant R, Banat MI. Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamni-lipid and micronutrients. Biores Technol. 2003; 90: 159-168.
25. Bako S, Chukwunonso D, Adamu AK. Bioreme-diation of refinery effluents by strains of Pseudo-monas aerugonosa and Penicillium janthinellum. Appl Ecol Environ Res. 2008; 6(3): 49-60.
How to Cite
Dutta, S.; Singh, P. Efficiency of Consortium for In-Situ Bioremediation and CO2 Evolution Method of Refines Petroleum Oil in Microcosms Study. European Journal of Biological Research 2017, 7, 97-107.
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