Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum

  • Nasim A. R. M. Othman Biology Department, Faculty of Science, Taiz University, Taiz, Yemen
Keywords: Amino acids, Antioxidant enzymes, Carbohydrates, Copper, Lipid peroxidation, Potassium, Proteins

Abstract

Here was investigated the incorporation of copper in MS medium on growth, and metabolic activities of Solanum nigrum callus. Copper up to 75 µM increased the growth, and thereafter a decline was observed. No considerable alteration in MDA, H2O2, bound phenolics, flavonoids, ascorbate, and copper content was observed with the existence of 25 µM copper, then levels of these parameters were raised with rising copper concentrations. Similarly, 25 µM copper didn't induce a considerable change in lipoxygenase, superoxide dismutase, catalase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase activities, however, high levels stimulated these enzymes. Copper at 25 µM didn’t considerably reduce amino acids and soluble proteins, whereas higher concentrations reduced these parameters. Copper treatments reduced the soluble carbohydrates accumulation; only 75 µM enhanced this accumulation. Copper at 25 µM significantly increased the potassium accumulation, whereas higher concentrations reduced this accumulation. From these results, it might be contemplated the optimum effect concerning copper.

DOI: http://dx.doi.org/10.5281/zenodo.2848646

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Published
2019-05-15
How to Cite
Othman, N. (2019). Optimization of copper for the improvement of in vitro plant tissue growth of Solanum nigrum. European Journal of Biological Research, 9(2), 77-92. Retrieved from http://www.journals.tmkarpinski.com/index.php/ejbr/article/view/168
Section
Research Articles