Effect of hormone for in vitro propagation of Asparagus racemosus Wild.

  • Niroj Paudel Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
  • Mukti Ram Aryal Department of Botany, Tri-Chandra multiple Campus (Tribhuvan University), Nepal
  • Rudra Hari Puri Department of Botany, Tri-Chandra multiple Campus (Tribhuvan University), Nepal
Keywords: Asparagus racemosus Wild., 6-benzyl amino purine (BAP), Growth and development, In vitro propagation, Medicinal plant, 1-napthalene acetic acid (NAA)


Asparagus racemosus Wild. is an undershrub, climber which is extremely branched with woody stem and spines like reduced leaves. It founds in tropical and sub-tropical region of Nepal up to 1500 meter above sea level. A. racemosus Wild. is an endangered species widely used in modern drug development and Ayurveda as well. The subculture explant shows responses to the different concentration of hormone. The composition of MS + 0.5 mg/l BAP and MS + NAA 0.5 mg/l + kinetin 1 mg/l most significant for shoot multiplication. Over them, the composition MS = 0.01 mg/l NAA + 1.0 mg/l BAP along with MS + 0.5 mg/l NAA = 1.5 mg/l kinetin is effective for the development of shoot in Asparagus racemosus. The hormone play important role on the regeneration of shoot in vitro condition. The knowledge of hormonal requirement help to promote the development and growth of endangered plants for their rapid propagation.

DOI: http://dx.doi.org/10.5281/zenodo.1407185


1. Malla SB, Shakya, PR. Vegetation and medicinal plants of Nepal. Nepal National Commission for UNESCO, Regional Seminar on the Ecology of Tropical Highlands. HMG and UNESCO. 1968: 1-10.

2. Shrestha UK, Shrestha KK. Some physio-chemical parameters of Asparagus racemosus from Nepal. In: Bulletin of the Department of Plant Resources, 2008.

3. Paudel N, Aryal MR, Das BD, Adhikari DC, Rai PD, Shrestha R. Some medicinal plant from Kathmandu Valley, Central Nepal. Int J Sci Rep. 2018: 4(4): 78-81.

4. Paudel N, Paudel LP, Ghimire U, Das BD. Archichlamydae and Sympetalae flora of Arghakanchi District, Western Nepal. Int J Life Sci Res. 2017: 5(3): 73-81.

5. Paudel N, Adhikari DC, Das BD. Some medicinal plants uses in ethnical group from Biratnagar, Eastern, Nepal. Am Sci Res J Engin Technol Sci. 2018; 41(1): 233-239.

6. Haberlandt G. Cultur versuche mit isolierten pflanzenzellen sitz. Ber Mat Nat ki Kais Akad Wiss, Wien. 1902; 111: 69-92.

7. Skoog F, Millar CO. Chemical regulation of growth and organ formation in plant tissue culture in in vitro. Syrap Sac Exp Biot. 1975; 11: 119-131.

8. Murashige T. Plant propagation by tissue culture: practice with unrealised potential. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS, eds. Handbook of plant cell culture. McGraw Hill Publishing, New York. 1990.

9. Kohmura H, Chokyu S, Harada T. Application of a new micropropagation system involving induction of bud clusters and somatic embryogenesis in Asparagus. Acta Horticult. 1996; 415: 119-128.

10. Schröder MB, Eimert K. Somatic embryogenesis in Asparagus. In: Quality enhancement of plant production through tissue culture (COST 843). Finland, 2000: 42-43.

11. Gupta S, Kumar A, Sharma SN. Improvement of seed germination in Asparagus racemosus Wild. J Herbs Spices Med Plants. 2002; 9(1): 3-9.

12. Saensouk P, Suddee N. No title. KKU Res J (Thailand). 2004: 31-39.

13. Pontarolli C, Camadro EL. Plant regeneration after long-term callus culture in clones of Asparagus officinalis L. Biocell. 2005; 29(3): 313-317.

14. Bopana N, Saxena S. Asparagus racemosus: ethnopharmacological evaluation and conservation needs. J Ethnopharmacol. 2007; 110(1): 1-15.

15. Dutta IC. Non timber forest product of Nepal. Hillside Press Ltd, Kathmandu Nepal. 2007: 44-45.

16. Bopana N, Saxena S. In vitro propagation of a high value medicinal plant: Asparagus racemosus Wild. In Vitro Cell Develop Biol. 2008; 44(6): 525-532.

17. Hurgoiu F, Blidar CF. Study regarding to Asparagus officinalis L. calus reaction, subcultured on aseptic medium with various growth regulators. Anal Univ Oradea Fasc Biol. 2008; XV.

18. Kumar A, Vijay N. In vitro plantlet regeneration in Asparagus racemosus through shoot bud differentiation on nodal segments. Science 2.0. 2009.

19. Pant, Krishna K, Joshi SD. Direct multiple shoot induction from the seed explants of wild Nepalese Asparagus racemosus Wild. Plant Arch. 2009; 9(1): 443-446.

20. Shrivastava N, Rajani M. Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell. Plant Cell Rep. 1999; 18(11): 919-923.

21. Rajkarnikar KM, Sainju HK, Bhatta GD. In vitro culture of Rauwolfia serpentine L. Benth. Ex. Kurz. Proc Nepal Japan Joint Symp. 2000: 232-234.

22. Ranjitkar KM, Bhatt GD, Adhikari MK. Micro propagation of Swertia ciliata. In: Scientific Publication Bull Dept. Plant Res. No. 24. Natural Product Research Laboratory, Thapathali, Kathmandu, Nepal. 2004: 10-12.

23. Paudel N, Paudel LP, Rai PD. Callus induction and shoot regeneration of Lycopersicon esculentum L. Int J Sci Res. 2017; 6(8): 1346-1348.

24. Aryal MR, Paudel N, Ranjit M. Elimination of Chhirkey and Foorkey viruses from meristem culture of large cardamom (Amomum subulatum Roxb.). Eur Online J Nat Social Sci. 2018; 7(2): 424- 442.
How to Cite
Paudel, N., Aryal, M., & Puri, R. (2018). Effect of hormone for in vitro propagation of Asparagus racemosus Wild. Current Life Sciences, 4(4), 53-61. Retrieved from http://www.journals.tmkarpinski.com/index.php/cls/article/view/81
Research Articles